The experiment is about the pH,
Buffers, as well as Concentration. Sometimes solutions are the homogeneous
mixtures, which is classified regarding to their gaseous, physical as well as
liquid states. The greatest interest for
the biologist is the liquid solutions because it is the most common solutions.
The solvent is the great quantity, where the component of the solutions is
present in this quantity. All biological systems which is the solvent is water.
In the solution, the components which is other than the solvents is known as
the solutes. In the solution the concentration of the solutes is explained in
the various methods, where the biologist is used the molarity to explain the
solutes concentrations in solvent by the mass percentages , “mole fraction” , “normality” plus the
“molarity” are the solution which is used in the other disciplines. The weak
electrolytes is the pure water because pure water is dissociate into the two
charged particles. The hydronium ions and the two hydroxide ions which is produced
through the reactions;
According to the above
equations, the number of hydronium ions in the pure water is always equal to
the hydroxide ions and it cannot be formed without it being made. In the pure
water the concentration of the hydroxide ions is and the concentration of the hydronium ions is . In the hydroxide as
well as hydronium ions, the pure water is actually the 0.0000001. When
this pure water molecules is dissociate, then the hydrogen atoms is the
covalently bond for the oxygen of the water molecules which leaves the
electrons for their hydrogen atom. This experiment which is used for the
anthocyanin extracted for the cabbage levees which has the intense of red color
in the pHs and it is colorless in order to pH in below figure
Material & Methods of Concentration, PH, and Buffers
During this experiment there are different
methods which is used to explain the experiment as discussed in the results
sections. The other method is the pH scale like the Richer Scale, which is used
to measure the strength of the earthquakes. In this lab experiment, the
material which is need is the Cyanidin, is in the red purplish anthocyanin and
the red cabbage. Where the hydroxyl group is labeled with the A, which convert
into the Cyanidin for bullish and purplish delphinine of the morning glory
flowers. Some of equipment and the material which is needed in this lab is that,
eight test tube with this racks.
Result of Concentration, PH, and Buffers
In the lab experiment it is
familiar by the terminology of pH. In the concentrations of pOHs as well as pH
and with labeled this is acidic or basic.
First of all work into the group,
then take the eight test tube along with the test tube rack then label it “2”,
“4”, “7”, “8”, “10”, “12”, and “14”. The use the re-pipettes which is deliver
the 5mL of the eights solution to pH and it has the corresponding number for this solution of pHs. Then use
this pipette, to transfer the 3ml of cabbage which extract the eight labeled
tubes, and its determine the color less stain of your clothes. Behind the every
tube place the white pieces which observed the color for the mixed solutions.
In the below table records the color which is observe in tube, and changes the
color gradually is shown in below table;
Tables
Table 1
pH of
Standard
|
Color of
Anthocyanin
|
Observations
|
2
|
Pink
|
|
4
|
Light pink
|
|
6
|
Light purple
|
|
7
|
Purple
|
|
8
|
Light blue
|
|
10
|
Green
|
|
12
|
Light green
|
|
14
|
Yellow
|
|
First of all work into the group,
then take the six test tube along with the test tube rack then label it “A”,
“B”, “C”, “D”, “E” and “F”. Then choose the six solution that is provided
there, and record the solution in the below table 2.
Table 2: pH of six household solution
Solution
|
Name
|
Color of
Anthocyanin
|
Estimated
pH
|
A
|
Aspirin
|
Pink
|
3
|
B
|
Antacid
|
green
|
10
|
C
|
Clear Soda
|
Light pink
|
3
|
D
|
Bleach
|
Light yellow
|
13
|
E
|
Vinegar
|
Dark pink
|
1
|
F
|
Apple Juice
|
red
|
3
|
There are six pieces of alkacid
is obtained, then return into it in bleach, and record the color of the
moistened paper in below table 3. Then compare the standard color and moistened
color which is provided for the alkacid in test paper then record the corresponding
pH;
Table 3: pH of six household solution
Solution
|
Name
|
Color of
Anthocyanin
|
Estimated
pH
|
A
|
Aspirin
|
Orange
|
3
|
B
|
Antacid
|
Light orange
|
5
|
C
|
Clear Soda
|
Orange
|
3
|
D
|
Bleach
|
Dark green
|
9
|
E
|
Vinegar
|
red
|
1
|
F
|
Apple Juice
|
Orange
|
3
|
Solution of the pipettor which is
used to transfer the 1mL of dilute solution that is containing phenolphthalein.
And record the pH of the solution in the below table;
Table 4: Buffered solution
Number of
Drops Added
|
Color of Phenolphthalein
|
pH of
solution
|
0
|
Colorless
|
6.8
|
3
|
Colorless
|
6.8
|
6
|
Colorless
|
6.8
|
9
|
Colorless
|
6.9
|
12
|
Colorless
|
6.8
|
15
|
Colorless
|
7
|
18
|
Colorless
|
7.1
|
21
|
Colorless
|
7.3
|
24
|
Pink
|
7.9
|
27
|
Dark pink
|
8.5
|
Table 5: Unbuffered Solution
Number of Drops
Added
|
Color of
Phenolphthalein
|
pH of
solution
|
0
|
Colorless
|
6.5
|
3
|
Colorless
|
7
|
6
|
Colorless
|
7.4
|
9
|
Pink
|
8
|
12
|
Dark pink
|
9
|
Graphs of
Concentration, PH, and Buffers
Buffer solution of Concentration, PH, and Buffers
Conclusion on Concentration, PH, and Buffers
Summing up all the discussions it
is concluded that, all the tables and the result of the lab experiment is
proper and accurate. The graph of the buffered solution along with the
un-buffered solution is properly labeled, by using the data which is given in
the table form. Different step is sued to explain the results, with the proper
tables was shown above in the results sections.
In different methods, the concentration
of the solute in a solution is explained, where the molarity is also explained the
concentration of solute in the solvents by the percentage of mass, where the normality,
mole fraction, and molarity is changes the other disciplines. By using the
following data which is given, in the table plot the graph of the buffer
solutions.