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Introduction of Spectrophotometric analysis of A mixture of absorbing substances

Category: Engineering Paper Type: Report Writing Reference: N/A Words: 250

In the present analysis, two different analytes are present in the solutions and each of the components has no significant impact on the light absorbing properties of these components. The absorption of light in the components is based on the wavelength and conditions. The strong interferences have an impact on the determination of the concentration (Skoog, et al., 2007). The spectrum of experiment demonstrates concentration. The ultimate purpose of the present work is the practical application of Beer-Lambert Law for the absorption calculation and to measure the ratio of each component. The working principle depends on the absorption properties of the material (Skoog, et al., 2007).

The spectrophotometer fires light beams of different wavelength and detects the logarithmic ratio of absorbance and transmission of light. The materials obey the Beer-Lambert law and it states that absorbance that is the logarithmic ratio for transmissivity of the light is directly proportional to the rate of concentration of the material. The extrapolating of absorbance defines the concentration of all the components in the material. It can be stated that the some of the absorbance of materials at a specific wavelength is equal to the absorbance of the whole mixture at the specified wavelength (Skoog, et al., 2007).  

Reference of Spectrophotometric analysis of A mixture of absorbing substances

Skoog, D. A., Holler, F. J. & Crouch, S. R., 2007. Principles of Instrumental Analysis. In: s.l.:Thomson Brooks/Cole, p. 1039.

 

 

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