In the
present analysis, two different analytes are present in the solutions and each
of the components has no significant impact on the light absorbing properties of
these components. The absorption of light in the components is based on the
wavelength and conditions. The strong interferences have an impact on the
determination of the concentration (Skoog, et al., 2007). The spectrum of
experiment demonstrates concentration. The ultimate purpose of the present work
is the practical application of Beer-Lambert Law for the absorption calculation
and to measure the ratio of each component. The working principle depends on
the absorption properties of the material (Skoog, et al., 2007).
The
spectrophotometer fires light beams of different wavelength and detects the
logarithmic ratio of absorbance and transmission of light. The materials obey
the Beer-Lambert law and it states that absorbance that is the logarithmic
ratio for transmissivity of the light is directly proportional to the rate of
concentration of the material. The extrapolating of absorbance defines the
concentration of all the components in the material. It can be stated that the
some of the absorbance of materials at a specific wavelength is equal to the
absorbance of the whole mixture at the specified wavelength (Skoog, et al., 2007).
Reference of Spectrophotometric analysis of A mixture of absorbing
substances
Skoog, D. A., Holler, F. J. & Crouch, S. R.,
2007. Principles of Instrumental Analysis. In: s.l.:Thomson Brooks/Cole, p.
1039.