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The
spectrophotometer works as an optical instrument and measures the intensity of
light in relation to the wavelength. In the simplified view, spectrophotometer evaluates
the light intensity of each color of light. The spectrophotometer works for the
energy or light in the visible region and the light is collected from the
sample and enters in the monochromatic by the aperture. The input light after
bouncing back from the material separates in different components of
wavelengths through the optical grating
The
light components separated through the optical grating is then focused on the
CCD array detector. The CCD is composed of thousands of pixels that detect
individual light patterns. The intensity of light is also measured that is
related to the spectrum of wavelength and light. Each compound in the spectrophotometer
transmits and absorbs the incident light over a range of wavelength. Spectrophotometer
evaluates the number of photons as the intensity of light after going through
the sample solution. The concentration of the chemical is determined by measuring
the intensity of light and depends on the wavelength of light sources
In
case of the visible region of the light, the complete electromagnetic spectrum
of light is different. Through the monochromator, the light separates in
rainbow featuring colors. In the spectrum, the blue pixels emit the blue light,
a green pixel emits green light and the red pixels emits the red portion of the
spectrum. The 508 PV microscope spectrophotometer includes UV VIs spectroscopy
that is related to the microscopic sample areas, thin film measurement,
colorimeter, an optical microscope.
UV visible spectrophotometer of A mixture of
absorbing substances
In the UV- VI
spectrophotometer light range is an ultraviolet range (185 400 nm), and the
wavelength range is between 400 – 700 nm.
IR spectrophotometer of A mixture of
absorbing substances
In the infrared range of
the electromagnetic radiation spectrum, the light range is 700 15000 nm.
Figure 2:
Color for the absorbed and reflected light in the spectrum
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