Building a microscope & collect images of various samples

Abstract of Building a microscope & collect images of various samples

The lab experiment is about the “Building the microscope and collects the different samples of the images.” In this experiment first of all the explanation is about the microscope, as shown in the introduction section. In this report there are approximately eight (8) sections, where as in the 1st section explain the introduction of the microscope and the image. J whereas both introductions are presented in the below introduction sections. Then in the introduction explain the objectives of the experiments, however as shown in the title build the microscope and collect the image samples. Then the second part of the report is the literature reviews, in this section studies of the different authors is explained about the microscope and the image.J.  Then the third part of the report is the experiment in these section different methods of the experiments, and the equipment which is used in this experiment is explained. Then the fourth part of the report is the Results in these section data tables, and the figures are explained. Then after this in the discussion part there is the comparison of the literature review, and the experimental and the results sections are shown. And in the conclusion section conclude all works that are learned from the lab experiments. And after this proved the future works and the recommendation of the work. At last add the references to give the proof and detailed of all works.     

  Keyword: Microscope, image.J. , TEM grid, Thor. Cam, CMOS

Introduction of Building a microscope & collect images of various samples

The term “Microscope” means micro refers the tiny, and the scope refers the look or view. The tool which is used to enlarge the images for small objects is known as the microscope. The amazing invention is the microscope, which is able to observe the specimen for the tiniest objects. The purpose of this lab experiment is how the microscopes function, and in the middle ages, the evolution of a microscope has been ongoing process [1].  

 

Figure 1: Microscope

Objectives of Building a microscope & collect images of various samples

·   In this experiment, the objective is that to collect the image of microscope, first of all build the microscope form the given components.

·   Then by using the TEM grid calibrate the scale 

Literature review of Building a microscope & collect images of various samples

According to the author [2], it is conducted that, for the TEM calibration it produces the wide ranges of the different samples of images where the high resolutions and the quantifications of results are required .Then offered the samples which are certified to maintain the highest possible preparation for the standard in production. The author [2] claibrations specimens for TEM are the renown for the worldwide with the realiability and the quality for each other which is also checked in the Electron microscope [2].

According to the author [3], it is conducted that the image.J is the Java based image processing software that is developed in the National institutes of health and the laboratory. This software is also run on the online applets which are downloadable applications. The image.J software could also display, edit and analyze, process the floating points of images. Whereas the list of the long plugins reflects the image.J and the usage by the range of the different applications in field of the engineering and sciences. The image.J is used in microscopy, medical imaging, and the material sciences but is not mentioned in the biological light microscopy. The image.J for the microscopy along with the accompanying of the manual which is developed and managed the wide array of the plugins. For new users of Image.J, analysis steps for microscopes and covering the typical image processing because the Image.J for Microscopy online manual is a useful resource [3] .

Experimental of Building a microscope & collect images of various samples

There are the following equipment which is used in this lab experiments;

·         Light Source

·         Slide holder

·         Objective lens

·         Camera (CMOS)

·         Cable

·         Laptop  

Now there is an explanation about this equipment, as the light passes through the light sources to various samples of the images, and the lens is magnified these sources at that time camera take the pictures which are connected with the laptop by the cable. Now by using the Thor.Cam, whereas its benefits are that we take the live picture by the Thor.Cam and then save this image whereas this image is open in the image.J software.

 Results of Building a microscope & collect images of various samples

Now according to the objective, collect the samples of the images, then it is obtained by using the Mesh, and it is the count of the number of opening in one of the line inches of the screen. This count is the mesh number, and 4 mesh screen means there are the four openings across the inch of the screen.

Actual values in the

For 6 pitches of Building a microscope & collect images of various samples

On the image.J software the microsphere image and TEM grid line is open, and then draws the line to calculate the pitch of the image. Determine the pitch in the  an also distance in the pixels which is also calculate on the scale.

Figure 2: 6 pitches

Area	Mean	Min	Max	Angle	Length
0.0.74	206.147	33.66	255	-19.33	7109
825.9	206.147	33.66	255	-19.33	750

 

Graph of Building a microscope & collect images of various samples

According to above graph it is about the 6 pitches which are determined by the Image.J software because the distance of the scale is given. There are two axes as shown in the above graph. As shown in the figure 2 the pitch is 300 aand in this above graph the y-axis is also shown value of 300.

For 9 pitches of Building a microscope & collect images of various samples

Figure 3 : 9 pitches

Area	Mean	Min	Max	Angle	Length
0.111	203.920	43.578	255	-109.3	10.602
1245.0	203.920	43.578	255	-109.3	1125

Graph of Building a microscope & collect images of various samples

According to below graph it is about the 9 pitches which are determined by the Image.J software because the distance of the scale is given. There are two axes as shown in the above graph. As shown in the figure 3 the pitch is 750 and in this below graph the y-axis is also shown value of 300.

To calibrate the samples, use the calibrations samples, whereas the samples consist of the 4 points with the different diameters like

 . Then put the slide in the sample holder, and take the image of every point. By using the different scales as given below, calculate the diameters of these points from the image J. Software.

For Calibration 1.5 mm

Figure 4: calibration 1.5 mm

Area	Mean	Min	Max	Angle	Length
1678.4	46.32	40.10	104	146.4	1527.6

For calibration 40.07 mm

Figure 5: calibration 40.07 mm

Area	Mean	Min	Max	Angle	Length
109.7	110	98.4	122.6	-2.54	98.925

For calibration 0.15 mm

Figure 6: calibration 0.15 mm

Area	Mean	Min	Max	Angle	Length
167.6	43.48	40.33	90.66	-92.49	151.67

For calibration 0.6 mm

 

Figure 7: calibration 0.6 mm

Area	Mean	Min	Max	Angle	Length
652.32	63.39	57.85	91.133	-94.46	592.56

Discussion of Building a microscope & collect images of various samples

According to the requirements of the experiments, its purpose is building the microscope and collects the image samples, for this, the translation stages which permit the samples to move away from the camera and the objective lens. The below figure should be used as the guide which keeps the setup straight , and the translation stages must be secured by using the screws.

Figure 8: Translation stages

Now in the below figure, the screw should be placed , where the micrometer screws must be facing in the opposite direction along with the two differed stages, which are placed as the separated through the 5cm on the same lines.

Figure 9: Screw placements  

Now in the discussion section, the comparison of the literature review and work which is done in this experiment is that the literature reviews explained the studied of the different authors.  

Conclusions of Building a microscope & collect images of various samples

Summing up all the discussion, it is concluded that the objective of the experiment is obtained. The microscope image and the TEM grid image should be obtained and open in the image.J software. All the results and the graphs with proper data in the tables are shown in the above discussion. Different calibration by using the TEM grid is also determined and shown in the above results sections. To build the microscope, the number of the pitches has different sizes which are also mentioned in the above results sections.  

Recommendations for future work

· Understand the capabilities and limitations of the microscope to get better results.

·    All the equipment must be neat clean.

References of Building a microscope & collect images of various samples

[1]	M. W. Davidson, "Introduction to Microscopy," 10 September 2019. [Online]. Available: https://micro.magnet.fsu.edu/primer/anatomy/introduction.html.
[2]	Agar, "CALIBRATION STANDARDS," 2019. [Online]. Available: http://www.agarscientific.com/tem/calibration-standards.
[3]	T. J. Collins, "ImageJ for microscopy," BioTechniques, vol. 43, no. 1S, p. S25–S30., 2007.