Loading...

Messages

Proposals

Stuck in your homework and missing deadline? Get urgent help in $10/Page with 24 hours deadline

Get Urgent Writing Help In Your Essays, Assignments, Homeworks, Dissertation, Thesis Or Coursework & Achieve A+ Grades.

Privacy Guaranteed - 100% Plagiarism Free Writing - Free Turnitin Report - Professional And Experienced Writers - 24/7 Online Support

A basic dye is utilized to stain bacterial cells

18/11/2021 Client: muhammad11 Deadline: 2 Day

Microbiology Lab Report- Gram Staining, Simple Staining, Negative Staining

1. Do a search online 1-2 antibiotics that affect Gram-positive bacteria and list them. On what part of the cell do the antibiotics usually work? List one or two antibiotics that affect Gram-negative bacteria? On what part of the cell do the antibiotics usually work? (Be sure to cite your sources in your answer.) (5 points)

2. Why do you think it is important to identify a bacterial disease in a patient before prescribing any antibiotic treatments? (Be specific.) (5 points)

3. What are some of the limitations of simple staining? (5 points)

4. Give an example of a situation in a lab or medical setting in which simple staining would be utilized. (5 points)

5. So far in this lab, you have used one type of simple stain(Crystal violet) and one type of negative stain (Nigrosin), yet there are many other simple and negative dyes available. Pick one simple dye and one negative dye, and discuss how those dyes differ from the ones you used in this lab. Give a scenario in which their use would be appropriate. (5 points)

6. Using either a textbook or a reputable online resource, research some of the typical characteristics of bacteria, and discuss why it might be important for a researcher or a hospital technician to be able to differentiate between Gram-positive and Gram-negative bacteria. (5 points)

Structure and Microscopy

Lab 4: Structure and Microscopy (100 points)

Student Name:

Student ID:

Course ID:
-Each question on the lab worksheet must be answered completely, thoroughly, in complete sentences and correctly in order to be considered for full credit
-If the question asks you to do research or find a source, a reputable, credible and/or scholarly source citation must be included in order to be considered for full credit
-If a math formula is required to arrive to an answer, work must be shown otherwise, no credit will be awarded
Pre-Lab Questions
1. What determines if a bacterial cell is Gram-positive or Gram-negative? (5 points)

Amount and location of the peptidoglycan molecule in the prokaryotic cell wall determines whether a bacterial cell is Gram-positive or Gram-negative.

2. In this lab, both viruses and prions were introduced as acellular organisms. Do some research and describe one other type of acellular organism. What characteristics about this organism classify it as acellular? (5 points)

Viroids are another type of acellular organism along with viruses and prions. They are plant pathogens, which consist only of a short strand of circular RNA capable of self-replication.

3. Bacteria have many different shapes that often determine their class. Research and form a hypothesis on the evolutionary reasons for so many different bacterial morphologies. (5 points)

Each bacterial morphology may be a selectable feature to aid survival and may have affected by different physical, environmental, and biological forces to contribute to natural selection.

Reference:

Young, K. D. (2006, September). The Selective Value of Bacterial Shape. Retrieved September 30, 2018, from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1594593/

4. Do a search online or look in your textbook for 1-2 antibiotics that affect Gram-positive bacteria and list them. On what part of the cell do the antibiotics usually work? List one or two antibiotics that affect Gram-negative bacteria? On what part of the cell do the antibiotics usually work? (Be sure to cite your sources in your answer.) (5 points)

5. Why do you think it is important to identify a bacterial disease in a patient before prescribing any antibiotic treatments? (Be specific.) (5 points)

d

Experiment 1 Results Tables
Table 1: Experiment 1 Staining Observations (5 points)

Stain used:

Crystal Violet

Observations:

Purple rod-shape bacteria with white background were observed

Experiment 1 Post-Lab Questions
1. How does crystal violet enhance the visualization of microbial features? (5 points)

Crystal violet enhances the contrast between the microorganism itself and the slide, making the bacteria appear as purple.

2. What are some of the limitations of simple staining? (5 points)

3. Give an example of a situation in a lab or medical setting in which simple staining would be utilized. (5 points)

Simple staining is used to obtain basic information about morphology of one type of microorganism through clear visualization.

Experiment 2 Results Tables
Table 2: Experiment 2 Staining Observations (5 points)

Stain used:

Nigrosin

Observations:

Background is stained, bacteria shows up as clear spiral.

Experiment 2 Post-Lab Questions
1. After visualizing the stained samples either using your microscope or by looking at the sample images provided, describe what physical/visual characteristics you were able to observe after performing the negative staining vs. after performing the simple stain. (5 points)

After looking at the sample images provided, negatively stained bacteria showed up as clear straight spirals against a dark background. Bacteria that are simple stained showed up as dark purple rods-shaped with white background.

2. So far in this lab, you have used one type of simple stain and one type of negative stain, yet there are many other simple and negative dyes available. Pick one simple dye and one negative dye, and discuss how those dyes differ from the ones you used in this lab. Give a scenario in which their use would be appropriate. (5 points)

Methylene blue is another dye that can be used for negative stain.

India Ink is another type of negative stain.

Experiment 3 Results Tables
Table 3: Experiment 3 Staining Observations (5 points)

Stain used:

Crystal violet (primary stain) & Safranin (counterstain)

Observations:

Gram-positive appeared as purple and Gram-negative showed up as pink.

Experiment 3 Post-Lab Questions
1. What color are the Gram-positive bacteria after Gram staining? Gram-negative bacteria? (5 points)

Gram-positive bacteria appear as dark purple or blue due to retaining the primary dye (Crystal Violet) in the cell wall.

Gram-negative bacteria appear as red or pink due to decolorizing to accept the counterstain (Safranin).

2. What different characteristic(s) exist between the two groups that account for the different staining conditions? (5 points)

Gram-positive bacteria are stained purple, and gram-negative bacteria stain as pink. They are two distinct morphological groups of bacteria.

3. Why was the Gram iodine added to the Gram staining procedure? (5 points)

Gram iodine is added as a mordant to stabilize the crystal violet iodine complex so that the dye cannot be removed easily.

4. Why is a counterstain (safranin) added to the Gram staining procedure? (5 points)

A counterstain is used to help identify gram-negative bacteria. Gram-negative bacteria lose the crystal violet and stain red.

5. What are the advantages of performing a Gram stain vs. a simple stain for visualizing bacteria? (5 points)

Gram stain contains two or more different stains and can differentiate the species of bacteria into two main groups (gram-positive and gram-negative) by looking at the color of cells (pink or purple). Simple stain involves single stain and it is used to easily determine cell shape, size, and arrangement.

6. Using either a textbook or a reputable online resource, research some of the typical characteristics of bacteria, and discuss why it might be important for a researcher or a hospital technician to be able to differentiate between Gram-positive and Gram-negative bacteria. (5 points)

7. Did you experience any technical difficulties or atypical results during this experiment? If so, what happened, and how could you avoid these issues in the future? (5 points)

Homework is Completed By:

Writer Writer Name Amount Client Comments & Rating
Instant Homework Helper

ONLINE

Instant Homework Helper

$36

She helped me in last minute in a very reasonable price. She is a lifesaver, I got A+ grade in my homework, I will surely hire her again for my next assignments, Thumbs Up!

Order & Get This Solution Within 3 Hours in $25/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 3 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

Order & Get This Solution Within 6 Hours in $20/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 6 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

Order & Get This Solution Within 12 Hours in $15/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 12 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

6 writers have sent their proposals to do this homework:

Professional Accountant
Phd Writer
Financial Solutions Provider
Helping Hand
Solutions Store
Assignment Guru
Writer Writer Name Offer Chat
Professional Accountant

ONLINE

Professional Accountant

This project is my strength and I can fulfill your requirements properly within your given deadline. I always give plagiarism-free work to my clients at very competitive prices.

$40 Chat With Writer
Phd Writer

ONLINE

Phd Writer

I will be delighted to work on your project. As an experienced writer, I can provide you top quality, well researched, concise and error-free work within your provided deadline at very reasonable prices.

$43 Chat With Writer
Financial Solutions Provider

ONLINE

Financial Solutions Provider

I have done dissertations, thesis, reports related to these topics, and I cover all the CHAPTERS accordingly and provide proper updates on the project.

$34 Chat With Writer
Helping Hand

ONLINE

Helping Hand

I reckon that I can perfectly carry this project for you! I am a research writer and have been writing academic papers, business reports, plans, literature review, reports and others for the past 1 decade.

$15 Chat With Writer
Solutions Store

ONLINE

Solutions Store

I have read your project description carefully and you will get plagiarism free writing according to your requirements. Thank You

$37 Chat With Writer
Assignment Guru

ONLINE

Assignment Guru

I will provide you with the well organized and well research papers from different primary and secondary sources will write the content that will support your points.

$43 Chat With Writer

Let our expert academic writers to help you in achieving a+ grades in your homework, assignment, quiz or exam.

Similar Homework Questions

Rocks on rosalind menu - The tennis court oath analysis - Application Essay - Business Intelligence (Assignmnet) - Vce japanese listening practice - Crypto Week 3 discussion - Jess's or jess grammar - Why is jules verne famous - According to economy from walden what happened before the framing - (Nursing) Teaching Plan Paper - Water by the spoonful by quiara alegría hudes pdf - Lone chooser method of fair division - Borachio much ado about nothing - File submission: Annotated Bibliography - Finger lickin good trademark - Research paper - Gene splicing virtual lab - Mathematical economics and econometrics pdf - Business process in sap fico - Due Tomorrow - Did target ignore data breach warnings - Modern english to shakespeare translation - Target market for portable chargers - How does cassio describe desdemona in act 2 - Journal-200 words - Power transformer oil specification - Smoke all day ka ikena ukulele chords - The maker's eye donald murray pdf - The cone gatherers summary of chapters - First choice ballymena jobs - Columbia yue ting company usa saber - Gm fuel pump connector - Ricon s series lift troubleshooting - Cloud Computing - Alaska fly fishing expedition case study - Residency Project - Chapter 11 international banking and money market - Beer vending machine tokyo - How to work out expected frequency - 100 spelling words for 5th grade - Rmit oua results release date - Sample persuasive speech on donating blood - What's the difference between firmware and software - Syrian War and EU in a vulnerable situation - Https lc ugrad1 gcu edu - Sex lies and conversation summary - Sunbeam fraud case - Executive summary of employee satisfaction - V for vendetta orchestra scene - Marcus king band in indianapolis the vogue november 15 - Pilot project evaluation template - Physical enablers in aged care - Journal 8 - Roman pottery identification guide - Bivariate relationship linearity strength and direction - Cosmic ferro alloys ltd credit rating - Under armour challenging nike in sports apparel case analysis - Principles of macroeconomics libby rittenberg and timothy tregarthen pdf - Fun home chapter 1 pdf - SCIENCE DISCUSSION(NO PLAGIARISM, A++ WORK, QUALITY, ON TIME) - Essays - Skin soap note - How long to fall 1km - Week 7 Discussion 2 - Mericans by sandra cisneros pdf - Robinia pseudoacacia frisia problems - Cyclic olefin copolymer chemical compatibility - Tkt module 3 past papers - Incident Response - Catholic social teaching caritas - Wk3 DQ1 Discussion Question 1 – CLO 2 - Continents and oceans lesson - Ibm deutschland gmbh 71137 ehningen - The lethal assumption close reading answers - Legal and ethical considerations for group and family therapy - Discussion and Assignment 7 - Financial statement with adjustment class 11 questions - Techniques in microbiology a student handbook pdf - Need 400- 500 words on the following topic - Hallidays functions of language - Anz capital notes 6 - Malabar shooting range opening hours - Reflection journal - Busi 600 - Product life cycle assignment - What mechanisms produce high precipitation at temperate latitudes - Speech about recycling plastic - Japan Automobiles - Discussion # 3- NUR601 - Christian Ethics Essay - Simplifying radicals worksheet doc - Mo co 6 pph3 - Culture and providing care to patients who do not speak english - Iphone repair burbank and whitsett - Mba 560 milestone one - Tertiary industry of chocolate - Brainology carol dweck annotated - Feast watson crackle lacquer - Case Study on Biomedical Ethics in the Christian Narrative - Pdf principles and characteristics of forensic accounting