Loading...

Messages

Proposals

Stuck in your homework and missing deadline? Get urgent help in $10/Page with 24 hours deadline

Get Urgent Writing Help In Your Essays, Assignments, Homeworks, Dissertation, Thesis Or Coursework & Achieve A+ Grades.

Privacy Guaranteed - 100% Plagiarism Free Writing - Free Turnitin Report - Professional And Experienced Writers - 24/7 Online Support

Api 20e analytical profile index database

15/10/2021 Client: muhammad11 Deadline: 2 Day

Objectives:

Learn how to perform and interpret the miniaturized, multi-test technique for bacterial identification.

Resource:

Lab manual: http://delrio.dcccd.edu/jreynolds/microbiology/2420/files/API%20%20RAPID%2020E.pdf

BioMerieux, Inc: https://apiweb.biomerieux.com

API 20E Instructions: http://biomanufacturing.org/uploads/files/587872707301898351-api20einstructions.pdf

Pearson MicroLab Tutor Video: API 20E

Introduction:

API-20E test strip (from bioMerieux, Inc.) is a standardized multi-test identification kit for Enterobacteriaceae family members which uses 21 miniaturized biochemical tests and a database (online software or codebook).

API 20 E (API = Analytical Profile Index)

Manufacturer: BioMérieux

Principle:

API test strip consists of 20 microtubes (wells) containing dehydrated testing media.

An unknown bacterial suspension is used to rehydrate and inoculate the media and the strip is incubated.

During incubation, biochemical reactions produce color changes, which are either spontaneous or revealed by the addition of reagents.

The test reactions are read according to the reading table, all + and - test results are compiled to obtain a profile number, which is then compared with the profiles in the codebook or uses online software to determine the identification of the bacterial genus and species.

(oxidase is not part of the strip but counts for the 21st test of API 20E - Enterobacteriaceae members are all negative)

Materials needed:

Setting up API 20E for inoculation and incubation:

Unknown bacterial specimen in agar plate

API 20E test strip

Sterile saline solution to make bacteria suspension

Sterile mineral oil to create anaerobioses for some tests

Reagents for reading API 20E after incubation:

Barrett's reagents A and B for VP test

Kovac's reagent for indole test

10% FeCl3 for TDA test

Pearson MicroLab Tutor Video: API 20E Oversized video in the file “API 20E.mpg” Please play in the file “API 20E.mpg” separately.

https://youtu.be/PXIis18qN9k

https://www.youtube.com/watch?v=ew3amo02_b0

More API videos:

Procedure:

1. Prepare a suspension of unknown bacteria in the saline tube.

Procedure continued:

2. Inoculate the API strip:

All wells - filled with bacteria suspension to the neck.

CIT, VP, and GEL - filled with more bacteria suspension to the top.

ADH, LDC, ODC, H2S, and URE - filled with sterile mineral oil to the top to create anaerobioses for the tests.

Procedure continued:

3. Incubate the strip in its chamber (tray):

Fill the divots of the tray with water to ensure the humidity for the microtubes not to dry out.

Place the strip into the tray.

Cover the chamber with the lid, and label it.

Incubate at 37oC for 18-24 hours.

4. Read test reactions according to the reading table after Incubation:

Add the test reagents to the wells:

1 drop of Barritt's A and B to VP (wait ~ 10 minutes to read)

1 drop of Kovac's to IND

1 drop of FeCl3 to TDA

Read all other tests without reagents.

Record all the results on the report sheet:

20 test reactions (+ or - ) from the strip

21st oxidase negative (-)

Procedure continued:

Case#1: Read the test reactions (+ or -) and record them on the report sheet for No.1 unknown bacterium. bacterium:

(answer key shown on the next page)

Interpretation:

Calculate 7-digit profile number based on all the 21 test results recorded:

Each three-test is grouped as section to calculate a single digit, assigning positive and negative results with specific scores, accordingly:

1st positive =1

2nd positive =2

3rd positive =4

negative =0

Add the three scores together as the single digit for this section.

For example:

“+ - +” section’s digit as 5 (1 + 0 + 4 = 5)

“+ + +” section’s digit as 7 (1 + 2 + 4 = 7)

21 tests are grouped into seven (21/3=7) sections, giving 7 digits in total as the 7-digit profile number for the bacterium.

No1. unknown bacterium’s

7-digit profile number: 5144572

Interpretation continued:

Input the 7-digit profile number onto BioMérieux website to identify the genus and species of the unknown bacterium.

No1. unknown bacterium’s 7-digit profile number: 5144572

Result:

Identification of No1. bacterium with 7-digit profile 5144572 is: Escherichia coli ---------- very good identification!

(genus) (species)

Case#2: No. 2 unknown bacterium’s 7-digit profile number: 5146472

Result:

Identification of No2. bacterium with 7-digit profile 5146472 is:

doubtfully narrowed to 3 possible organisms

----- need additional test(s) for the final identification.

Case#3: No. 3 unknown bacterium’s 7-digit profile number: 5537352

Result:

Identification of No3. bacterium with 7-digit profile 5537352 is:

unacceptable profile ----- error.

In summary

API 20E strip is used in the identification of Enterobacteriaceae family members.

The result is interpreted by means of a 7-digit profile number, which can be looked up in a database.

API 20E advantages:

Easy to use: Multi-test identification kit.

Rapid identification ~ 24-hour identification of Enterobacteriaceae family members.

API 20E disadvantages:

likely only give a broad outline, additional tests are often necessary for the final identification.

may fail to give any result due to systemic or personal errors.

Checkpoints:

What are the advantages of API 20E test (compared to conventional identification methods)?

What are the disadvantages of API 20E test?

Calculate the 7-digit profile number for this report sheet.

Homework is Completed By:

Writer Writer Name Amount Client Comments & Rating
Instant Homework Helper

ONLINE

Instant Homework Helper

$36

She helped me in last minute in a very reasonable price. She is a lifesaver, I got A+ grade in my homework, I will surely hire her again for my next assignments, Thumbs Up!

Order & Get This Solution Within 3 Hours in $25/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 3 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

Order & Get This Solution Within 6 Hours in $20/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 6 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

Order & Get This Solution Within 12 Hours in $15/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 12 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

6 writers have sent their proposals to do this homework:

Chartered Accountant
Top Rated Expert
Top Writing Guru
Homework Master
Engineering Solutions
Quick Finance Master
Writer Writer Name Offer Chat
Chartered Accountant

ONLINE

Chartered Accountant

This project is my strength and I can fulfill your requirements properly within your given deadline. I always give plagiarism-free work to my clients at very competitive prices.

$29 Chat With Writer
Top Rated Expert

ONLINE

Top Rated Expert

I have done dissertations, thesis, reports related to these topics, and I cover all the CHAPTERS accordingly and provide proper updates on the project.

$46 Chat With Writer
Top Writing Guru

ONLINE

Top Writing Guru

I find your project quite stimulating and related to my profession. I can surely contribute you with your project.

$40 Chat With Writer
Homework Master

ONLINE

Homework Master

I have assisted scholars, business persons, startups, entrepreneurs, marketers, managers etc in their, pitches, presentations, market research, business plans etc.

$30 Chat With Writer
Engineering Solutions

ONLINE

Engineering Solutions

I am an academic and research writer with having an MBA degree in business and finance. I have written many business reports on several topics and am well aware of all academic referencing styles.

$40 Chat With Writer
Quick Finance Master

ONLINE

Quick Finance Master

I am an experienced researcher here with master education. After reading your posting, I feel, you need an expert research writer to complete your project.Thank You

$19 Chat With Writer

Let our expert academic writers to help you in achieving a+ grades in your homework, assignment, quiz or exam.

Similar Homework Questions

Tulane university accounts payable office - Centre for clinical interventions worry - Comma on ti 84 - Topic 3 review - Bachelor of pharmaceutical science honours - Production cost analysis and estimation applied problems - 2-3 pages - Information system case analyze - Major steps in the implementation process - Will provide upon request - Book study space ucl - Pearson and gallagher 1983 citation - Nhs net pop3 settings - What physical property most distinguishes biotite mica from muscovite mica - Essay - Fantastique bikes is a company that manufactures bikes - Essay needed - Worksheet on separating mixtures - Evidence Based Capstone Project - Edelweiss pirates cologne 1944 - Ftp swfwmd state fl us - Homework controversy new york times - Whats half of a teaspoon - Hp envy 750qd desktop pc - History of tomato sauce mother sauce - Mgt 3810 - Target market of jollibee - Weddings & Events - Ikea vidga rail triple track - LOCAL, STATE, FEDERAL LEVELS OF LAW ENFORCMENT ASSIGNMENT CJT101 - Writing Assignment #2 Comparing and Contrasting Two Websites - Wm wrigley jr company case analysis - Furry friends foundation i creating a new database from scratch - Keynows - Solidworks equation syntax error - Cloud Computing - Duplicity theory of vision - Types of strategies ppt - Final project / presentation slide - The dba creates security policies and procedures. - Beef cattle lesson plans high school - Left sphenoid wing meningioma icd 10 - Before the flood movie worksheet answer key - Legal and ethical considerations for group and family therapy - Motec m800 wiring diagram - Odwalla e coli lawsuit - U.S. UNIIONS - Themes in billy elliot - Kinetic and potential energy lab - Case study - Why are African Americans and other people of color overrepresented among the working poor? Have affirmative action programs helped some people move out of this category? Why or why not? - Hercules allusion in hamlet - Something wicked this way comes quote - Java software solutions etc w bind in access edition 8th - Kip in to kn m - Mystery of history timeline figures - What is the vascular shunt mechanism - Robert b marks origins of the modern world pdf - How do i write a play script - What do colors mean in wireshark - Acrostic words for teacher - Features of international financial market - North lakes state collage - Customers experiencing allergic reactions often show which symptom - Meta Themes Draft - Fun geography games for the classroom - Do geologists lick rocks - Discussion 11 - X 4y x 2y 12 - Mylabsplus montclair state university - Care plan for nutritional needs - What happens in macbeth act 1 scene 3 - Individual success plan - Discussion Post 4-1 - Agl new connections ewr - Ashes in the water nursery rhyme - Encoding Presidential Gender - What does chingaso mean in spanish - They say i say 4th edition chapter 7 summary - 2016 hsc business studies - Michael kimmel bros before hos - 91 lanita road ferny grove - Week three discussion - What does reconation mean - InfoTech in a Global Economy - Walden nurs 6630 final exam - Hills home hub price list - Pacific Culture Picture Powerpoint - The birds by tarjei vesaas - Date table 1 anion confirmation tests - Getty art history information program - James woodall boston massacre - Emory hospital clinical trials - ASAP just about 2 paragraphs - 140 lbs to kg - Monash academic safety net - Voltage probe in ltspice - Three ways in which risks are measured - Advocate role in social work - 4 types of innovation invention extension duplication synthesis