Loading...

Messages

Proposals

Stuck in your homework and missing deadline? Get urgent help in $10/Page with 24 hours deadline

Get Urgent Writing Help In Your Essays, Assignments, Homeworks, Dissertation, Thesis Or Coursework & Achieve A+ Grades.

Privacy Guaranteed - 100% Plagiarism Free Writing - Free Turnitin Report - Professional And Experienced Writers - 24/7 Online Support

Api 20e quick index booklet

20/03/2021 Client: saad24vbs Deadline: 7 Days

Learn how to perform and interpret the miniaturized, multi-test technique for bacterial identification.

Resource:

Lab manual: http://delrio.dcccd.edu/jreynolds/microbiology/2420/files/API%20%20RAPID%2020E.pdf

BioMerieux, Inc: https://apiweb.biomerieux.com

API 20E Instructions: http://biomanufacturing.org/uploads/files/587872707301898351-api20einstructions.pdf

Pearson MicroLab Tutor Video: API 20E

Introduction:

API-20E test strip (from bioMerieux, Inc.) is a standardized multi-test identification kit for Enterobacteriaceae family members which uses 21 miniaturized biochemical tests and a database (online software or codebook).

API 20 E (API = Analytical Profile Index)

Manufacturer: BioMérieux

Principle:

API test strip consists of 20 microtubes (wells) containing dehydrated testing media.

An unknown bacterial suspension is used to rehydrate and inoculate the media and the strip is incubated.

During incubation, biochemical reactions produce color changes, which are either spontaneous or revealed by the addition of reagents.

The test reactions are read according to the reading table, all + and - test results are compiled to obtain a profile number, which is then compared with the profiles in the codebook or uses online software to determine the identification of the bacterial genus and species.

(oxidase is not part of the strip but counts for the 21st test of API 20E - Enterobacteriaceae members are all negative)

Materials needed:

Setting up API 20E for inoculation and incubation:

Unknown bacterial specimen in agar plate

API 20E test strip

Sterile saline solution to make bacteria suspension

Sterile mineral oil to create anaerobioses for some tests

Reagents for reading API 20E after incubation:

Barrett's reagents A and B for VP test

Kovac's reagent for indole test

10% FeCl3 for TDA test

Pearson MicroLab Tutor Video: API 20E Oversized video in the file “API 20E.mpg” Please play in the file “API 20E.mpg” separately.

https://youtu.be/PXIis18qN9k

https://www.youtube.com/watch?v=ew3amo02_b0

More API videos:

Procedure:

1. Prepare a suspension of unknown bacteria in the saline tube.

Procedure continued:

2. Inoculate the API strip:

All wells - filled with bacteria suspension to the neck.

CIT, VP, and GEL - filled with more bacteria suspension to the top.

ADH, LDC, ODC, H2S, and URE - filled with sterile mineral oil to the top to create anaerobioses for the tests.

Procedure continued:

3. Incubate the strip in its chamber (tray):

Fill the divots of the tray with water to ensure the humidity for the microtubes not to dry out.

Place the strip into the tray.

Cover the chamber with the lid, and label it.

Incubate at 37oC for 18-24 hours.

4. Read test reactions according to the reading table after Incubation:

Add the test reagents to the wells:

1 drop of Barritt's A and B to VP (wait ~ 10 minutes to read)

1 drop of Kovac's to IND

1 drop of FeCl3 to TDA

Read all other tests without reagents.

Record all the results on the report sheet:

20 test reactions (+ or - ) from the strip

21st oxidase negative (-)

Procedure continued:

Case#1: Read the test reactions (+ or -) and record them on the report sheet for No.1 unknown bacterium. bacterium:

(answer key shown on the next page)

Interpretation:

Calculate 7-digit profile number based on all the 21 test results recorded:

Each three-test is grouped as section to calculate a single digit, assigning positive and negative results with specific scores, accordingly:

1st positive =1

2nd positive =2

3rd positive =4

negative =0

Add the three scores together as the single digit for this section.

For example:

“+ - +” section’s digit as 5 (1 + 0 + 4 = 5)

“+ + +” section’s digit as 7 (1 + 2 + 4 = 7)

Homework is Completed By:

Writer Writer Name Amount Client Comments & Rating
Instant Homework Helper

ONLINE

Instant Homework Helper

$36

She helped me in last minute in a very reasonable price. She is a lifesaver, I got A+ grade in my homework, I will surely hire her again for my next assignments, Thumbs Up!

Order & Get This Solution Within 3 Hours in $25/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 3 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

Order & Get This Solution Within 6 Hours in $20/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 6 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

Order & Get This Solution Within 12 Hours in $15/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 12 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

6 writers have sent their proposals to do this homework:

Top Essay Tutor
Top Writing Guru
Assignment Hut
Essay & Assignment Help
Write My Coursework
Finance Homework Help
Writer Writer Name Offer Chat
Top Essay Tutor

ONLINE

Top Essay Tutor

Give me a chance, i will do this with my best efforts

$32 Chat With Writer
Top Writing Guru

ONLINE

Top Writing Guru

You can award me any time as I am ready to start your project curiously. Waiting for your positive response. Thank you!

$37 Chat With Writer
Assignment Hut

ONLINE

Assignment Hut

I am known as Unrivaled Quality, Written to Standard, providing Plagiarism-free woork, and Always on Time

$23 Chat With Writer
Essay & Assignment Help

ONLINE

Essay & Assignment Help

I will cover all the points which you have mentioned in your project details.

$37 Chat With Writer
Write My Coursework

ONLINE

Write My Coursework

I will cover all the points which you have mentioned in your project details.

$19 Chat With Writer
Finance Homework Help

ONLINE

Finance Homework Help

Hello, I an ranked top 10 freelancers in academic and contents writing. I can write and updated your personal statement with great quality and free of plagiarism

$36 Chat With Writer

Let our expert academic writers to help you in achieving a+ grades in your homework, assignment, quiz or exam.

Similar Homework Questions

1000 WORD MIN ESSAY DUE 11/1 THREE SCHOLARLY SOURCES/ APA format - Island man poem questions - Project management for engineering business and technology fifth edition pdf - Advertising copywriting and layout - Townsendpress.net sign in - Mcdonald's general environment - Project evaluation and review technique pert - What approaches could have yielded additional valuable information - The aliens annie baker plot summary - C&EW6D - Cisco switch configuration commands ppt - 8 bandicoot lane mylor - Me talk pretty one day exploring the text answers - Levi's personal pair jeans case study pdf - Xi gua shi ping - 937 941 victoria road west ryde nsw 2114 - Dr pepper snapple group annual report 2017 - Homework - Connor corporation hired a new accountant - Logos in julius caesar - Case Study - 540 paper - Red mountain multigenerational center hours - Average fixed costs diminish continuously as output increases - Journal 7 - Mythbusters mentos and coke - Bar mark shape codes - Grade calculator in python - 1723 sturt st ballarat - Benchmark - Professional Capstone and Practicum Reflective Journal (TOPIC Fall prevention strategies in hospitals.) - Misplaced affections discharge for sexual harassment - Azure reserved vm instances are an example of opex - Green zone conversation book - If ever you disturb our streets again - Legrand wide slide dimmer installation - Suppose that xtel currently is selling - Lord clarke of hampstead - Importance of tessellations in life - Employee goal setting template - 19 moods of categorical syllogism - What does kika mean in hawaiian - Amadeus availability display explanation - Sipvicious scanner detection palo alto - Jb hi fi mobile byo plan $65 12 month - 1 var stats ti 84 meaning - Watch film, summarize and answer question - How does place value help me divide - How many chromosomes does a zebronkey have - A difficult pregnancy a nurse practitioner looks for answers - Cloud security alliance (csa) cloud controls matrix spreadsheet - Discussion Question - What is the purpose of a dlci - Shabdam bharatanatyam dance mp3 - I m not scared essay - Different drawing materials instrument tools and equipment - Graph of sinx and cosx - Evidence based practice - Kim cheng boey stamp collecting - Daniel radcliffe raps periodic table - Greasers and socs similarities - Builds productive relationships selection criteria - Tacrolimus for pannus in dogs - Amdr for polyunsaturated fat - Assignmnet - Osha electrical safety toolbox talk - Overview chemical bonds worksheet - Dunn inc is a privately held furniture manufacturer - Conceptual dependency graph in artificial intelligence - Overhead catenary system design - Love is easy chords - Canadian hospitality law 3rd edition - Preparation of standard potassium permanganate solution - Cisco 500-560 exam dumps - Trade off analysis decision making - The outdoor furniture corporation manufactures two products - The wall street journal classroom edition chapter 1 answers - Urgent 1- 7 hours due - Lewis v averay [1972] 1 qb 198 - Chart of roman numerals - Barometer used for measuring atmospheric pressure - In the opening vignette, predictive modeling is described as - Capital investment decisions case study - Grand canyon university student teaching requirements - Experiment 1 observation of mitosis in a plant cell - Single pole double throw relay diagram - Argumentative essay on mobile phones in school - Source document for petty cash book - Ikea moon light recall - Sample soap note for diabetes - Bernie madoff case study - How to calibrate rosemount absolute pressure transmitter - Around 1000 words - The history of vogue - The fashion channel case study solutions - Foxx and azrin toilet training article - How many km is in a light year - Create an advertising campaign assignment - Many customers get their first impression of your company from - 9 - Imoprtance of strategic IT planning - Answer questions