Loading...

Messages

Proposals

Stuck in your homework and missing deadline? Get urgent help in $10/Page with 24 hours deadline

Get Urgent Writing Help In Your Essays, Assignments, Homeworks, Dissertation, Thesis Or Coursework & Achieve A+ Grades.

Privacy Guaranteed - 100% Plagiarism Free Writing - Free Turnitin Report - Professional And Experienced Writers - 24/7 Online Support

Api 20e quick index booklet

20/03/2021 Client: saad24vbs Deadline: 7 Days

Learn how to perform and interpret the miniaturized, multi-test technique for bacterial identification.

Resource:

Lab manual: http://delrio.dcccd.edu/jreynolds/microbiology/2420/files/API%20%20RAPID%2020E.pdf

BioMerieux, Inc: https://apiweb.biomerieux.com

API 20E Instructions: http://biomanufacturing.org/uploads/files/587872707301898351-api20einstructions.pdf

Pearson MicroLab Tutor Video: API 20E

Introduction:

API-20E test strip (from bioMerieux, Inc.) is a standardized multi-test identification kit for Enterobacteriaceae family members which uses 21 miniaturized biochemical tests and a database (online software or codebook).

API 20 E (API = Analytical Profile Index)

Manufacturer: BioMérieux

Principle:

API test strip consists of 20 microtubes (wells) containing dehydrated testing media.

An unknown bacterial suspension is used to rehydrate and inoculate the media and the strip is incubated.

During incubation, biochemical reactions produce color changes, which are either spontaneous or revealed by the addition of reagents.

The test reactions are read according to the reading table, all + and - test results are compiled to obtain a profile number, which is then compared with the profiles in the codebook or uses online software to determine the identification of the bacterial genus and species.

(oxidase is not part of the strip but counts for the 21st test of API 20E - Enterobacteriaceae members are all negative)

Materials needed:

Setting up API 20E for inoculation and incubation:

Unknown bacterial specimen in agar plate

API 20E test strip

Sterile saline solution to make bacteria suspension

Sterile mineral oil to create anaerobioses for some tests

Reagents for reading API 20E after incubation:

Barrett's reagents A and B for VP test

Kovac's reagent for indole test

10% FeCl3 for TDA test

Pearson MicroLab Tutor Video: API 20E Oversized video in the file “API 20E.mpg” Please play in the file “API 20E.mpg” separately.

https://youtu.be/PXIis18qN9k

https://www.youtube.com/watch?v=ew3amo02_b0

More API videos:

Procedure:

1. Prepare a suspension of unknown bacteria in the saline tube.

Procedure continued:

2. Inoculate the API strip:

All wells - filled with bacteria suspension to the neck.

CIT, VP, and GEL - filled with more bacteria suspension to the top.

ADH, LDC, ODC, H2S, and URE - filled with sterile mineral oil to the top to create anaerobioses for the tests.

Procedure continued:

3. Incubate the strip in its chamber (tray):

Fill the divots of the tray with water to ensure the humidity for the microtubes not to dry out.

Place the strip into the tray.

Cover the chamber with the lid, and label it.

Incubate at 37oC for 18-24 hours.

4. Read test reactions according to the reading table after Incubation:

Add the test reagents to the wells:

1 drop of Barritt's A and B to VP (wait ~ 10 minutes to read)

1 drop of Kovac's to IND

1 drop of FeCl3 to TDA

Read all other tests without reagents.

Record all the results on the report sheet:

20 test reactions (+ or - ) from the strip

21st oxidase negative (-)

Procedure continued:

Case#1: Read the test reactions (+ or -) and record them on the report sheet for No.1 unknown bacterium. bacterium:

(answer key shown on the next page)

Interpretation:

Calculate 7-digit profile number based on all the 21 test results recorded:

Each three-test is grouped as section to calculate a single digit, assigning positive and negative results with specific scores, accordingly:

1st positive =1

2nd positive =2

3rd positive =4

negative =0

Add the three scores together as the single digit for this section.

For example:

“+ - +” section’s digit as 5 (1 + 0 + 4 = 5)

“+ + +” section’s digit as 7 (1 + 2 + 4 = 7)

Homework is Completed By:

Writer Writer Name Amount Client Comments & Rating
Instant Homework Helper

ONLINE

Instant Homework Helper

$36

She helped me in last minute in a very reasonable price. She is a lifesaver, I got A+ grade in my homework, I will surely hire her again for my next assignments, Thumbs Up!

Order & Get This Solution Within 3 Hours in $25/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 3 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

Order & Get This Solution Within 6 Hours in $20/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 6 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

Order & Get This Solution Within 12 Hours in $15/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 12 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

6 writers have sent their proposals to do this homework:

Top Essay Tutor
Top Writing Guru
Assignment Hut
Essay & Assignment Help
Write My Coursework
Finance Homework Help
Writer Writer Name Offer Chat
Top Essay Tutor

ONLINE

Top Essay Tutor

Give me a chance, i will do this with my best efforts

$32 Chat With Writer
Top Writing Guru

ONLINE

Top Writing Guru

You can award me any time as I am ready to start your project curiously. Waiting for your positive response. Thank you!

$37 Chat With Writer
Assignment Hut

ONLINE

Assignment Hut

I am known as Unrivaled Quality, Written to Standard, providing Plagiarism-free woork, and Always on Time

$23 Chat With Writer
Essay & Assignment Help

ONLINE

Essay & Assignment Help

I will cover all the points which you have mentioned in your project details.

$37 Chat With Writer
Write My Coursework

ONLINE

Write My Coursework

I will cover all the points which you have mentioned in your project details.

$19 Chat With Writer
Finance Homework Help

ONLINE

Finance Homework Help

Hello, I an ranked top 10 freelancers in academic and contents writing. I can write and updated your personal statement with great quality and free of plagiarism

$36 Chat With Writer

Let our expert academic writers to help you in achieving a+ grades in your homework, assignment, quiz or exam.

Similar Homework Questions

Read the following material and answer questions - Adobe photoshop lightroom 4 serial number - How to ask for feedback - The cry of the children poem - Ealth Promotion Project Outline - Plus one web series - Design an abstract class named bank account - DISCUSSION - Description of your project (Required): - Case Study - Sdtm ig 3.1 3 pdf free download - Fundamentals of human resource management 4th edition mcgraw hill - 4ps of innovation space model - Essay Due Tonight - Why is dell's darker deep blue color trademarked - Human resource management lepak 3rd edition - Health assessment - Efficiency of simple machines - Acute disease effect score - Aig’s ceo, edward liddy, demonstrated his lack of personal commitment to high ethical standards by - Final project - Write an essay about the poem "The love song of J.Alfred Prufrock" - Faye abdellah patient centered approach to nursing - Malcolm in the middle water park - Wk 2 - Chapt 5 Research Assignment: Qual vs Quant - Horney styles of relating - Macbeth act 2 questions - Transpose of matrix maple - The habitable planet ecology lab answers - Programming c++ code for ticket parking - Venn diagram set notation - How to make a mummy national geographic - Probability - Fish cheeks by amy tan answer key - Comprehensive career program - Public house os map symbol - They say i say across disciplines pdf - The shabbat by marjane satrapi - Management information system assignment question - Devon bishop age 45 is single - Auditing a risk based approach edition - Healthcare policy in the US - Project development and implementation for strategic managers assignment - Abstract algebra a first course by dan saracino - Method of drawing the line in ethics - An eminent aerospace engineer into the wild - Robert graves goodbye to all that sparknotes - Celf 5 sample report - Manifest destiny paper - Partnership - Australian scanner frequencies victoria - Management Challenges - Adams water cartage bendigo - Foreign state threat - Professional Associations Membership - The life stages of an individual saver include which of the following: - What is the role of a human service worker - Database systems - Buchan caves lakes entrance - Walden university mission statement - Vnu business publications bv - Decision by blades inc to invest in thailand - An uncomfortable bed summary - Mezirow's transformative learning theory - Expressio unius est exclusio alterius pronunciation - Week 4 health care - Carrefour case study answers - Bolted fault current calculation - Milestone 4 - ME - Main - Homework 1 & 2 - 10 - Revision (essay) - Affordable Care Act Research - Permissible bending stress in steel - Junior doctor training program - Nurs 6051 week 11 assignment - Vitamin water revive label morse code - Quantitative research proposal example - Cmgtt 111 - Deltav m-series wiring diagram - Helmet manufacturing plant cost - David sedaris tickets state theatre of ithaca april 5 - Dunder mifflin hierarchy - How to write about limitations of a study - The sash my father wore chords - Super bill of materials - Melody of certain damaged lemons zip - Professional digital portfolio. - Virginia History - Economics quiz 2 - Habitable zones lab answers - What is emotive language - Kennards lift and shift rocklea - 3 p of business communication - Www math odu edu bogacki lat - ESSAY - Mood of the persistence of memory - Macy's inventory turnover - Holy sonnets death be not proud by john donne - Example of biotic community