Loading...

Messages

Proposals

Stuck in your homework and missing deadline? Get urgent help in $10/Page with 24 hours deadline

Get Urgent Writing Help In Your Essays, Assignments, Homeworks, Dissertation, Thesis Or Coursework & Achieve A+ Grades.

Privacy Guaranteed - 100% Plagiarism Free Writing - Free Turnitin Report - Professional And Experienced Writers - 24/7 Online Support

Api biomerieux apiweb

28/03/2021 Client: saad24vbs Deadline: 2 Day

Learn how to perform and interpret the miniaturized, multi-test technique for bacterial identification.

Resource:

Lab manual: http://delrio.dcccd.edu/jreynolds/microbiology/2420/files/API%20%20RAPID%2020E.pdf

BioMerieux, Inc: https://apiweb.biomerieux.com

API 20E Instructions: http://biomanufacturing.org/uploads/files/587872707301898351-api20einstructions.pdf

Pearson MicroLab Tutor Video: API 20E

Introduction:

API-20E test strip (from bioMerieux, Inc.) is a standardized multi-test identification kit for Enterobacteriaceae family members which uses 21 miniaturized biochemical tests and a database (online software or codebook).

API 20 E (API = Analytical Profile Index)

Manufacturer: BioMérieux

Principle:

API test strip consists of 20 microtubes (wells) containing dehydrated testing media.

An unknown bacterial suspension is used to rehydrate and inoculate the media and the strip is incubated.

During incubation, biochemical reactions produce color changes, which are either spontaneous or revealed by the addition of reagents.

The test reactions are read according to the reading table, all + and - test results are compiled to obtain a profile number, which is then compared with the profiles in the codebook or uses online software to determine the identification of the bacterial genus and species.

(oxidase is not part of the strip but counts for the 21st test of API 20E - Enterobacteriaceae members are all negative)

Materials needed:

Setting up API 20E for inoculation and incubation:

Unknown bacterial specimen in agar plate

API 20E test strip

Sterile saline solution to make bacteria suspension

Sterile mineral oil to create anaerobioses for some tests

Reagents for reading API 20E after incubation:

Barrett's reagents A and B for VP test

Kovac's reagent for indole test

10% FeCl3 for TDA test

Pearson MicroLab Tutor Video: API 20E Oversized video in the file “API 20E.mpg” Please play in the file “API 20E.mpg” separately.

https://youtu.be/PXIis18qN9k

https://www.youtube.com/watch?v=ew3amo02_b0

More API videos:

Procedure:

1. Prepare a suspension of unknown bacteria in the saline tube.

Procedure continued:

2. Inoculate the API strip:

All wells - filled with bacteria suspension to the neck.

CIT, VP, and GEL - filled with more bacteria suspension to the top.

ADH, LDC, ODC, H2S, and URE - filled with sterile mineral oil to the top to create anaerobioses for the tests.

Procedure continued:

3. Incubate the strip in its chamber (tray):

Fill the divots of the tray with water to ensure the humidity for the microtubes not to dry out.

Place the strip into the tray.

Cover the chamber with the lid, and label it.

Incubate at 37oC for 18-24 hours.

4. Read test reactions according to the reading table after Incubation:

Add the test reagents to the wells:

1 drop of Barritt's A and B to VP (wait ~ 10 minutes to read)

1 drop of Kovac's to IND

1 drop of FeCl3 to TDA

Read all other tests without reagents.

Homework is Completed By:

Writer Writer Name Amount Client Comments & Rating
Instant Homework Helper

ONLINE

Instant Homework Helper

$36

She helped me in last minute in a very reasonable price. She is a lifesaver, I got A+ grade in my homework, I will surely hire her again for my next assignments, Thumbs Up!

Order & Get This Solution Within 3 Hours in $25/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 3 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

Order & Get This Solution Within 6 Hours in $20/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 6 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

Order & Get This Solution Within 12 Hours in $15/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 12 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

6 writers have sent their proposals to do this homework:

Financial Analyst
Homework Master
Study Master
Calculation Guru
Quick Finance Master
Top Writing Guru
Writer Writer Name Offer Chat
Financial Analyst

ONLINE

Financial Analyst

Hello, I an ranked top 10 freelancers in academic and contents writing. I can write and updated your personal statement with great quality and free of plagiarism

$56 Chat With Writer
Homework Master

ONLINE

Homework Master

I will cover all the points which you have mentioned in your project details.

$45 Chat With Writer
Study Master

ONLINE

Study Master

I am known as Unrivaled Quality, Written to Standard, providing Plagiarism-free woork, and Always on Time

$56 Chat With Writer
Calculation Guru

ONLINE

Calculation Guru

I am known as Unrivaled Quality, Written to Standard, providing Plagiarism-free woork, and Always on Time

$40 Chat With Writer
Quick Finance Master

ONLINE

Quick Finance Master

Give me a chance, i will do this with my best efforts

$89 Chat With Writer
Top Writing Guru

ONLINE

Top Writing Guru

Hello, I an ranked top 10 freelancers in academic and contents writing. I can write and updated your personal statement with great quality and free of plagiarism

$45 Chat With Writer

Let our expert academic writers to help you in achieving a+ grades in your homework, assignment, quiz or exam.

Similar Homework Questions

Protein synthesis translation diagram labeled - Rashtriya uchchatar shiksha abhiyan rusa - Compute stanley black & decker's gross profit margin - BUSN601 - Class c amplifier operation - Discuss the use of cloud backup strategies - Hydrogen gas is collected by water displacement - PACE 111 T 6983 - Project management simulation scope resources and schedule - It 145 milestone one pseudocode - O brien auto group onemint login - Climate change - Drama passive aggressive behavior - Intern Paper CAL2 - Ezra pound make it new - Ammonium hydroxide and silver nitrate chemical equation - Acc 202 presentation to investors - Car pulling trailer physics problem - A bar of steel has the minimum properties - Benefits of integrity in the bible - Suppose the quantity x of super titan - Rob sitch jane kennedy wedding - La trobe university academic referencing tool - Aws direct connect gateway icon - Gcse french writing holidays example - Animal farm important quotes - Https miic health state mn us - Public dividend select fund - Discussion Board - Why rinse buret with naoh before titration - 904 n grand ave waxahachie tx 75165 - Reread this sentence from the letter from birmingham jail - Ibm signature selling method - Ecosystems organisms and trophic levels virtual lab - Great is he chords - Wk 2 - Apply: Signature Assignment: Statistical Report - What is the present value of the following annuity - Brave new world feelies chapter - Warlow v harrison case summary - Which itil process uses mean time between failures mtbf - Discussion 3 - Research strategies badke 5th edition pdf - 3 highview crescent coolaroo - Brisbane city council gym green square - 8 to 1 multiplexer ic - Mildura visitor information centre - Discussion 8 - Answer two discussion questions - Sierra company incurs the following - Www science teachers com earth htm answers - I need a discussion for week 9 and a response to to other classmates for my Marketing Class. - Aunt alexandra being racist - Bayonet charge poem analysis - Evaporation and intermolecular attractions lab answers - Block Chain Development discussion 7 - Ac relay coil snubber - Paper - Lippitt's phases of change - Assignment: Technology and Terrorism - Neighbourhood watch lally katz analysis - East west university grading system - National Labor Relations Act - What word does jefferson use to describe rights - Progressivism's foremost educational advocate was - St andrews village tamworth - Everybody look left everybody look right lyrics - At the airport dialogue - Encryption Responses - Las vegas relative location - New manufacturing facility in china case study - Need Help ASAP 2 Part work - Microbial Journal Review Article - Blood spatter analysis lab - Cu dubh translation - Physical Security Assessment - please make sure the instructions listed in the question are fulfilled - Socw 6456 - Ftk lab - Computer Crime - Personal development blueprint - What time will it be in 65 minutes - Ist presentation - App development wbs - Kel mcnaughton carving jig - Citizens national bank macomb il - Learning to read by frances ellen watkins harper analysis - Computer Science - Windshield assessment example - Thinking through the past volume 1 chapter summary - Calculator texas scientific ti-30xb multiview - What is the rimland of the caribbean - Lenze plc designer license - The university of sydney application - Sample marketing plan of cement company - The universe secrets of the sun - Revays auto service harrisburg pa - Power amplifier efficiency calculation - Threats attacks and vulnerability assessment - Macbeth act 1 scene 1 annotations - Repertoire definition to kill a mockingbird - How to put a deutsch connector together