Loading...

Messages

Proposals

Stuck in your homework and missing deadline? Get urgent help in $10/Page with 24 hours deadline

Get Urgent Writing Help In Your Essays, Assignments, Homeworks, Dissertation, Thesis Or Coursework & Achieve A+ Grades.

Privacy Guaranteed - 100% Plagiarism Free Writing - Free Turnitin Report - Professional And Experienced Writers - 24/7 Online Support

Bacterial and fungal amylase lab report

05/01/2021 Client: saad24vbs Deadline: 24 Hours

“The Effect of Temperature on Amylase Activity”


Methods


The objective of this experiment was to determine the effect of temperatures (0 °C, 25 °C, 55 °C, and 85 °C) on fungal (Aspergillus oryzae,) and bacterial amylase’s ability to break down starch. Our team monitored starch catalysis using the Iodine test. Iodine turns the most catalyzed starch yellow, and the least catalyzed blue. When the reaction turns blue-black or dark brown, it means that starch is present and, therefore, not catalyzed.


Each member of team four had a temperature assigned to work with. Each individual monitored the activity of amylase (fungal and bacterial.) Time also varied respectively ( 0 mins, 2 mins, 4 mins, 6 mins, 8 mins, and 10 mins.) The group marked the spot plates with time (on the side) and temperatures (across the top.) The team labeled 4 test tubes with different temperatures, the enzyme source (B or F) and the group’s number (4.) The group also marked the pipette for each temperature. Test tubes were placed into the respective temperatures. Finally, the team carefully carried out the two trials.


Without removing the test tubes from the water bath at different temperatures, the team added 2 drops of starch from each temperature to the first row of the spot plate (at 0 minutes) containing iodine. The team poured the same amount of iodine before each trial. The team proceeded, transferring the starch into the amylase (fungal and bacterial) at their respective water bath and mix these two without removing the test tubes from the water bath. Then, the team added two drops of this new mixture (starch-amylase) at their respective temperatures to the second row of the spot plate. The other rows were filled with the same mixture at the temperatures assigned with two minutes interval between each trial.


Results


The following tables and pictures show the results group four (first) and all groups (last) obtained.


Group 4 Result




Data Collected for all Groups


Fungal Amylase vs. starch




Bacterial Amylase vs. starch




After conducting the whole experiment, the team collected the data for the results. The group observed that the least amount of starch was present at 55 °C at any time, for bacterial amylase and 25°C to 55 °C for fungal amylase (for most students.) The reasoning behind this statement might be that the mixtures at 55 °C turned light yellow in the presence of iodine (at all times except time 0) for bacterial amylase. The mixture became dark yellow (at all times except time 0) at temperatures 25 °C to 55 °C for fungal amylase, which could indicate that amylase activity was not affected. The team also noticed that the enzyme amylase denatures at higher temperatures (85 °C) for both types of amylase. The group made that assumption because the solution turned dark blue brown at 85 °C at all times. According to The Iodine test, dark color indicates the starch is present and not catalyzed.


The average of starch concentration of all groups at each temperature for fungal amylase demonstrates that there are no outsiders in our data and that the results’ standard deviation is not significantly big for the experiment concluded. The results obtained for all the trials suggest that amylases (bacterial or fungal) have a respective optimum temperature. It is also evident that if these amylases are not placed on their ideal temperature, it loses their ability to break starch down.


Guerra 2


Guerra 2

Homework is Completed By:

Writer Writer Name Amount Client Comments & Rating
Instant Homework Helper

ONLINE

Instant Homework Helper

$36

She helped me in last minute in a very reasonable price. She is a lifesaver, I got A+ grade in my homework, I will surely hire her again for my next assignments, Thumbs Up!

Order & Get This Solution Within 3 Hours in $25/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 3 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

Order & Get This Solution Within 6 Hours in $20/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 6 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

Order & Get This Solution Within 12 Hours in $15/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 12 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

6 writers have sent their proposals to do this homework:

Top Essay Tutor
University Coursework Help
Helping Hand
Writer Writer Name Offer Chat
Top Essay Tutor

ONLINE

Top Essay Tutor

I have more than 12 years of experience in managing online classes, exams, and quizzes on different websites like; Connect, McGraw-Hill, and Blackboard. I always provide a guarantee to my clients for their grades.

$105 Chat With Writer
University Coursework Help

ONLINE

University Coursework Help

Hi dear, I am ready to do your homework in a reasonable price.

$102 Chat With Writer
Helping Hand

ONLINE

Helping Hand

I am an Academic writer with 10 years of experience. As an Academic writer, my aim is to generate unique content without Plagiarism as per the client’s requirements.

$100 Chat With Writer

Let our expert academic writers to help you in achieving a+ grades in your homework, assignment, quiz or exam.

Similar Homework Questions

Examples of effective interdepartmental communication within an organisation - Cuso4 5h2o percent composition - The house at sugar beach chapter summaries - Breezypythongui - Llc net income and statement of members equity - How to find planned investment spending - Books to read for accounting and finance personal statement - WEEK VIII PT 2A - Project management - Windows server deployment proposal - Etruscan artisans particularly excelled at which of the following - UNIT DISCUSSION 2: Chapters 3 and 4 (week 3 and 4): Cognitions and Brain (development)... - Wk7 DQ1 Discussion Question 1 – CLO 5 - Young writers monster poetry - Normal probability plot minitab - Research project - Reason relativity and responsibility in computer ethics - Zoot suit play summary - Hpq hpe spinoff cost basis - Luminaire file for dialux evo - Russian steam locomotive 4-14-4 - Psf to kg m2 - Nature/nurture - Business Intelligence - Rossi and hall muon experiment - Which of the following statements typifies defined contribution plans? - How to get white crease lines out of jeans - Need 500+ words with no plagiarism in APA 7 Format - Bedtime sticker chart printable - How to write an investigative report essay - The logic of stupid poor people summary - Certified general accountants of ontario - Australian unity remedial massage rebate - Mohegan tribe kevin brown red eagle - Water by the spoonful full pdf - Sedimentary rock flow chart - Business Research Methodology - Health Promotion & Disease Prevention in Older Adults - Hopper ants adelaide hills - Criminal Law - Isbn 10 1 305 38983 2 - Psychological testing and assessment aiken pdf - Deferred Taxes Project Assignment - Advanced Financial Reporting DUE Sunday, September 26, 2020 @ 3pm ET - Case study healing and autonomy - Dateline a crack in everything youtube - West meon parish records - A polar bear starts at the north pole - Discussion question, 350 words and 2 references. - Family assessment paper for social work - Phil week 2 - Vector lab report physics - Freemium case study - Microwave frequency band designation - A personal statement detailing evidence of academic rehabilitation and/or changed circumstances to prove the likelihood of your future academic success - A paper on Mercedes Benz Vision AVTR Concept, Advanced technologies & impact on Automobile industry - Hey google talk to headspace - CUSTOMER INTERVIEWS - Lsl and usl meaning - Loddon district scout shop - Indo european family tree - Ceres gardening company - Discussion 10 - Nissan micra k11 obd2 - Empirical formula of oxalic acid - Conclusion ohm's law lab report - Access crosstab query multiple column headings - Refugee and special humanitarian proposal form 842 - What are the dimensions of a2 paper in cm - Tp fastt poetry analysis chart - How to build bridges with friends - Ellis crane hire perth - Pan europa foods case study answers - Cessna citation excel interior - According to the textbook, the central theme of the wisdom literature is: - Hoyt sector model city examples - The parable of the prodigal son characters - Marketing Research - Why might you use a perception check - Literature Evaluation - Conflicts in the outsiders chapter 1 - Common anode 7 segment display circuit - Young scot kidz card - The great gatsby body paragraph - Scaled instruments for mass - Financial Management Assignment. - Essay Knowledge, Expert and Content management systems - What factors are involved in shaping a child’s personality? Discuss in reference to concepts drawn from at least two theories of personality. - Elm leaf beetle natural control - No nonsense extra class study guide - Reign of error diane ravitch pdf - Excel 2019 In Practice - Ch 9 Independent Project 9-5 - Microsoft access reserved words - What does rcdd stand for - 1.06 macbeth character development - Juvenile rheumatoid arthritis slideshare - Warnerwoods company uses a perpetual inventory system - Sbcglobal email password reset - Appointment with death cast - Ikea resources and capabilities - Freud id ego superego