Brief reflections on childhood trauma and society bruce d perry

Adding a Scale Bar to Images Taken with the Leica Fluorescent MicroscopeUsing Built in Software (Leica Application Suite) –more convenient for a couple imagesTake an image –Aquire tab -> Acquire ImageSwitch to Process tabSelect your image from the dashboard at the bottom of the screenSwitch to “Calib.” Sub-tabClick “Manual (Draw Line)”Enter the “pixels” and “length” for the objective you used according to the chart below:Select microns as the unitClick “Apply”Switch to “Annotate” sub-tabIn the Scale Bar tab, click “Show”You can now click and drag the scale bar to place it wherever you pleaseYou may also change the length by selecting “User Length” and typing a new valueFormat the font and colors in the “font” boxIn the “Actions” box, click “Merge”Right click on the large image, click “Save As”Save the image with scale bar to you folder.Using ImageJ –Easier for many imagesSave all images with the magnification in the name or on the folder!!!(ex: 5x, 10x...)Download ImageJ: http://rsb.info.nih.gov/ij/download.htmlInstall ImageJOpen the first picture file into ImageJ: ctrl+O (or) file -> openSet Scale: Analyze -> Set Scale...Enter pixel and distance values and unit of length according to table below:Lens MagnificationDistance in PixelsKnown DistanceUnit of LengthLeica 5x107200μmLens MagnificationPixelsLengthUnit of LengthLeica 5x107200μmLeica 10x107100μmLeica 20x438200μmLeica 40x431100μmLeica 63x680100μm
Leica 10x107100μmLeica 20x438200μmLeica 40x431100μmLeica 63x680100μmCheck the Global length box!Click: “Analyze” -> “Tools...”-> “Scale Bar...”Enter in the desired length of scale bar in the “Width in” boxFormat the bar with the following boxesYou can now quickly add scale bars to your other pictures:Open imageClick: “Analyze” -> “Tools...”-> “Scale Bar...”Format scale barIf you clicked the “global length” box, you will not have to set the scale for each image until you edit an image that used a different objective.If you do not know the “Distance in pixel” or “known distance”Get a measuring standard slide (or mm ruler for the dissecting microscopes).Take a picture, record the objective magnification as well as any projector lenses between the objective and camera.Open picture in ImageJZoom in on the standardization lines with the magnifying glass tool (10thicon on tool bar)Use the line tool (5thicon on tool bar) and draw a line along the known length of the standardization lines (hint: hold shift to constrain the line to right angles)Go to Set Scale: “Analyze” -> “Set Scale...”The value in “distance in pixels” is your “Distance in Pixels” and the standardization length is the “known distance”