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main article:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3143600/
This paper is based on a cysteine labeling technique originally detailed in this paper
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2741095/
address the following 4 questions in your write-up:
1. Explain how the cysteine labeling technique works. Why does it not label buried cysteines?
2. Explain the significance of fibronectin unfolding under mechanical load. What is a reason this would be beneficial?
3. What kinds of protein bonds would be disrupted by mechanical forces?
4. Extra cytseines were introduced into the protein. Briefly explain how this was done. Explain the control experiment that was performed to prove that the cysteines did not disrupt protein folding
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