Loading...

Messages

Proposals

Stuck in your homework and missing deadline? Get urgent help in $10/Page with 24 hours deadline

Get Urgent Writing Help In Your Essays, Assignments, Homeworks, Dissertation, Thesis Or Coursework & Achieve A+ Grades.

Privacy Guaranteed - 100% Plagiarism Free Writing - Free Turnitin Report - Professional And Experienced Writers - 24/7 Online Support

Cellular respiration fermentation in yeast lab report

03/12/2021 Client: muhammad11 Deadline: 2 Day

BIOL 102: Lab 7

Yeast Fermentation

PRE-LAB ASSIGNMENT:

Students are expected to read pages 1-2 before coming to the lab to complete the experiments.

Print this entire lab packet and bring it to the laboratory.

Please provide a FULL lab report for this experiment following the “Lab Report Guidelines”.

Please note that this lab report WILL include a HYPOTHESIS.

Objectives:

· Observe yeast fermentation

· Determine the optimum conditions for yeast fermentation

Background:

All fungi are eukaryotes. Although they vary in size and shape, fungi share key characteristics including their way of obtaining nutrients for growth and energy. Fungi are heterotrophs and they depend on preformed carbon molecules produced by other organisms. However, fungi do not ingest food and then digest it using enzymes; instead they invade -think of a moldy piece of bread-a food source and secrete digestive enzymes onto it. The digestion occurs outside the body. When the polymers are broken down into monomers, the fungi absorb the predigested food into its body.

Yeast are microscopic, unicellular organisms in the Kingdom Fungi. Like other fungi, yeast are incapable of making their own food, but like any other organism, need food for energy. They rely on carbohydrates (usually sugars) found in their environment to provide them with this energy so that they can grow and reproduce. There are many species of yeast, and each has a particular food source.

Regardless of the food source, yeast perform fermentation which does not utilize oxygen. In fermentation, the only energy extraction pathway is glycolysis, with one or two extra reactions tacked on at the end, but no electron transport chain. Therefore, only 2 ATPs are formed per glucose.

Fermentation and cellular respiration begin the same way, with glycolysis. In fermentation, however, the pyruvate made in glycolysis is not completely oxidized because it does not continue through the citric acid cycle and the electron transport chain does not run. Because the electron transport chain is not functional, the NADH cannot drop its electrons off to the electron transport chain, and thus very few ATP molecules are synthesized because the ATP synthase is not running.

Based on the end products, fermentation can be of two types: ALCOHOLIC fermentation (the subject of this lab) and LACTIC ACID fermentation.

image1.png

Regardless of the type of fermentation, the purpose of the extra reactions in fermentation, is to regenerate (recycle) the electron carrier NAD+ from the NADH produced in glycolysis. The extra reactions accomplish this by letting NADH drop its electrons off with an organic molecule such as acetaldehyde to produce ethanol (alcoholic fermentation), or pyruvate to produce lactic acid (lactic acid fermentation). This “drop-off” of electrons allows glycolysis to keep running by ensuring a steady supply of NAD+.

Going from pyruvate to ethanol is a two-step process. In the first step, a carboxyl group is removed from pyruvate and released as carbon dioxide, producing a two-carbon molecule called acetaldehyde. In the second step, NADH passes its electrons to acetaldehyde, regenerating NAD+ and forming ethanol.

Yeast breaks down glucose into ethanol, 2 carbon dioxide molecules, and 2 ATP molecules. The formula for the yeast fermentation reaction is:

Reactant Products

C6H12O6 >>>>>>> 2CH3CH2OH + 2CO2 + 2 ATP molecules

For the yeast cell, this chemical reaction is necessary to produce the energy for life. The ethanol and the carbon dioxide are waste products. It is these waste products that we take advantage of: we use the ethanol in alcoholic beverages and the carbon dioxide makes bread rise when baking.

Alcoholic fermentation, can be observed and measured by using the amount of carbon dioxide gas that is produced from the breakdown of glucose. In this exercise, you will observe alcoholic fermentation by yeast. To do so you will add the same amounts of yeast and water to different amounts of sugar in Erlenmeyer flasks and cap them with a balloon to see how much carbon dioxide gas is produced. You will also use water at two different temperatures and determine how much carbon dioxide is produced. The more fermentation that occurs, the more carbon dioxide will be produced, and the more the balloon will expand.

Information adapted from:

Solomon, Eldra P. et al. Biology. 10th ed. Cengage, 2015.

https://www.khanacademy.org/science/biology/cellular-respiration-and-fermentation

LAB DATASHEET

Determine the optimum conditions for yeast fermentation.

Think Scientifically:

Please explain your rationale to which flask or test variable will produce the most CO2. Look at the various bottles below and state whether bottle A-F will produce the most CO2 and explain why.

Materials:

Sugar

Dry yeast

Warm water

Ice cold water

Balance scale

Measuring spoons

100 mL Graduated Cylinder

6 Erlenmeyer flasks

6 Rubber bands

6 Balloons

Ruler

Procedure:

1. Obtain 6 labeled Erlenmeyer flasks.

2. Fill each flask accordingly:

· Bottle A - 5 mL sugar, 3 grams of dry yeast

· Bottle B - 10 mL sugar, 3 grams of dry yeast

· Bottle C - 15 mL sugar, 3 grams of dry yeast

· Bottle D - 5 mL sugar, 3 grams of dry yeast

· Bottle E - 3 grams of dry yeast

· Bottle F - 15 mL sugar

3. Fill all flasks except D with 100 mL of warm water. Fill flask D with 100 mL of ice cold water.

4. Place a balloon over the top of each flask and tighten it with a rubber band.

5. Swirl flask to mix contents. Wait 20-30 minutes.

6. Record observations in Table 1.

7. Measure the width and height of the balloon (from the top of the flask to the top of the balloon) with a ruler, and record it in Table 1.

8. Graph the Sugar Quantity vs. Balloon Height in an X-Y Scatterplot. Insert DIGITAL scatterplot only. Written graphs and/or pictures of written graphs will not be accepted.

Table 1: Observations and Measurements of Balloon height in cm

Flask

Observations

Height

Width

A

1st to rise

4.5inch

2inch

B

3rd to rise

3.8inch

1.5inch

C

2nd to rise

4.2inch

1.8inch

D

Did not rise

0

0

E

Did not rise

0

0

F

Did not rise

0

0

Conclusion:

Be sure to address the following:

· How did your original rationale compare to the data collected? If your rationale was incorrect, why do you think it did not produce the most CO2?

· Describe what happened in this reaction using the following terms: yeast, warm water, cold water, sugar, anaerobic respiration, and carbon dioxide.

· Compare what happened to each of the balloons for flasks A through F. Which flask had the most CO2 production? Least? How do you know? Be sure to describe WHY!

· There were four experimental flasks and two control flasks in this exercise. Which flasks were the experimental and which were the control flasks? Explain how each determination was made.

Homework is Completed By:

Writer Writer Name Amount Client Comments & Rating
Instant Homework Helper

ONLINE

Instant Homework Helper

$36

She helped me in last minute in a very reasonable price. She is a lifesaver, I got A+ grade in my homework, I will surely hire her again for my next assignments, Thumbs Up!

Order & Get This Solution Within 3 Hours in $25/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 3 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

Order & Get This Solution Within 6 Hours in $20/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 6 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

Order & Get This Solution Within 12 Hours in $15/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 12 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

6 writers have sent their proposals to do this homework:

Solution Provider
Top Grade Essay
Homework Tutor
Quality Homework Helper
WRITING LAND
Academic Master
Writer Writer Name Offer Chat
Solution Provider

ONLINE

Solution Provider

I find your project quite stimulating and related to my profession. I can surely contribute you with your project.

$32 Chat With Writer
Top Grade Essay

ONLINE

Top Grade Essay

I have done dissertations, thesis, reports related to these topics, and I cover all the CHAPTERS accordingly and provide proper updates on the project.

$50 Chat With Writer
Homework Tutor

ONLINE

Homework Tutor

I find your project quite stimulating and related to my profession. I can surely contribute you with your project.

$42 Chat With Writer
Quality Homework Helper

ONLINE

Quality Homework Helper

I am an academic and research writer with having an MBA degree in business and finance. I have written many business reports on several topics and am well aware of all academic referencing styles.

$48 Chat With Writer
WRITING LAND

ONLINE

WRITING LAND

I am an elite class writer with more than 6 years of experience as an academic writer. I will provide you the 100 percent original and plagiarism-free content.

$16 Chat With Writer
Academic Master

ONLINE

Academic Master

I am an experienced researcher here with master education. After reading your posting, I feel, you need an expert research writer to complete your project.Thank You

$39 Chat With Writer

Let our expert academic writers to help you in achieving a+ grades in your homework, assignment, quiz or exam.

Similar Homework Questions

How big is a mole project - The following information is available for aikman company - Titration of hydrogen peroxide with potassium permanganate - 12 angry men video questions - Ieee journal of photovoltaics abbreviation - South east wales centre for teacher education and training - Warr company is considering a project - 5 7 driving data answers - A company using the accrual method of accounting performed - Suitcase measurements length width height - Mcdonalds philippines case study - Appraisal cost and prevention cost - De nobili toscani longs - Urbancode deploy rest api - Marketing Strategies - Milestone PAPER 3 - APPLE - 12 pulse rectifier waveform - Handbook of informatics for nurses and healthcare professionals 6th ed - IOT security - Word - Calculate angular momentum of earth - Professional Ethics and Responsibilities at work ( IT software job ) - History of Art through the Middle Ages (Project) - Ethical issue - Transportation then and now - Cloud computing - Lesley university bursar - Insights Into Criminal Behavior - Free selection criteria examples answers - What is a value scale in art - Managerial economics and organizational architecture 4th edition pdf - An example of conduction - Oomph goo and stain remover ingredients - Pursuit Of Happiness - Weisbord's six box model strengths weaknesses - Greetings from bury park pdf - Extron cable cubby 500 cut out template - Internal and external factors affecting marketing mix - Java program to create bank account - NEED DISCUSSION IN 18 HOURS or LESS - Exponential and logarithmic functions test answers - Answer the following question in 600 to 700 words - Thermal energy harvesting power supply w ltc3108 and supercapacitor storage - Fish cheeks answer key pdf - 5 Ways to Find Easy Topic for Your Essay - Nichols and Gehart& Tutttle textbooks - Battery room lighting requirements - Final strategic plan and presentation powerpoint - Earthquake elko nv - Examples of systems in everyday life - Clematis hagley hybrid rhs - How many minutes is 86400 seconds - Cond mat journal club - Aami safe driver rewards ruby - Victoria halls newcastle university - Fig plucker tongue twister - What is long shore drift - The grinch who stole christmas poem analysis - E30 brake pad replacement - Game development project proposal - Www newadvent org rosary - Cherry tree cattery wickford - Broadcast spawning advantages and disadvantages - EDU - The mixed up chameleon - Entity type and entity instance - Death be not proud by john donne pdf - Role and Scope Research 1 - Calculate volume of a box in litres - Turkeys in the kitchen analysis - Thermodynamics2 - Nyt the real campus scourge - Optimum custhelp app answers list 136 138 search router - Statins stat case study answers - What is merge activity in project management - Bob beck brain tuner schematic - Snhu hum 100 project 1 - Jerry lee lewis house booneville ms - Who warned, “advertisements contain the only truth to be relied on in a newspaper?” - Experimental methods - Difference between assignment and delegation - B what makes up the rungs of the dna molecule - Civic writing project urgent! - Diels alder reaction lab - How many moles are in of aspartame - Soler non verbal communication - How to curate wikis on amino - Example of nursing process case study - 6 steps of root cause analysis ihi - The wife's story analysis and comprehension questions answers - Mosfet gate and source shorted - Analytical profile index test - Ψ meaning in biology - Credit4all - Hatchet chapter 17 summary - Internal and External Audiences - Discussion B week 8 - How to double the volume of a square pyramid - Www macmillanenglish com inspiration 3 - J trading full circle outsourcing - Dunkin donuts market share 2015