Procedure 2 Focusing the Microscope Now that we know how to handle the microscope properly, let's practice using i. 1 Obtain a slide of the letter "e. 2 Examine the letter "e"slide macroscopically before placing it on the stage. How is the "e" oriented on the slide? Is it right side up, upside down, backward, etc.? 3 Ensure the nosepieceis switched to low che slide eand ureMor the slide using the stage-adjustment knob until the "e"is in your field of view. 4 Use the coarse-adjustment knob to bring the slide into focus slowly. After it is grossly in focus, use the fine-adjustment knob to sharpen the focus. How is the "e"oriented in the field of view? Is it different from the way it was when you examined it in step 22 5 Move the nosepiece to medium power. You should only have to adjust the focus with the fine-adjustment knob; no adjustment of the coarse focus should be necessary. After you have examined the slide on medium powes,move the nosepiece to high powe. Again, focus only with the fine-adjustment knob. Wasn't that easy? 6 Procedure 3 Depth of Focus At times you will look at a slide and seesomething brown or black and kind of neat looking with intcresting swirls and specks. What is this fascinating discovery you've made? It's dirt on top of the slide. This happens cause people tend to focus the objective on the first thing they can make out, which usually is the top of the coverslip the slide, which has a tendency to be dirty the depth of focus is the thickness of a specimen in sharp focus. Thicker specimens will require you to focus up and n to look at all levels of the specimen. This takes practice and skill. Let's get some practice doing this These "dirt discoveries" can be avoided by appreciating what is known as depth of focus. Also called the depth of d, Obtain a slide with three colored threads. The threads are located on the slide at varying depths, and you will have