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Data table 1 chromatogram data

25/12/2020 Client: saad24vbs Deadline: 3 days

Copyright 2019 - Hands-On Labs | http://holscience.com 1 / 5


Student Name


Mohammed Alnaimi


Student ID 48408 Lesson Chromatography of Food Dyes Institution Ocean County College Session 2019L3 CHEM 182 DL1 Course CHEM 182 DL1 Instructor Nancy Marashi


Final Report


Exercise 1


CHEM 182 DL1 Chromatography of Food Dyes


There will be a separation of com ponents of ink. This could create an error with the com ponents that are trying to separate. This said, a pen could not be used.


1. What might happen if a student used a pen to mark the baseline on the chromatography paper?


The solution will m ove to the top and you would not be able to calculate the Rf value that is com pared to the distance that a com ponent traveled in the stationary phase reached by the solvent.


2. What would happen if the student did not stop the development before the solvent reached the top of the paper?


The solvent would wash away the dye.


3. What would happen if the solvent level was above that of the dye spots on the paper at the start of the development?


Copyright 2019 - Hands-On Labs | http://holscience.com 2 / 5


Photo 1: Chromatogram


Data Table 1: Chromatogram Data


Because the solvents used for chrom atography are volatile. If it is not m arked im m ediately, there is a possibility that it m ay evaporate and then you would not be able to draw the solvent.


4. Why is it important to mark the solvent level on the chromatography paper as soon as you remove it f rom the Petri dish?


Sample Solvent front (mm)


Blue 1


Blue 2


Red 3


Red 40


Yellow 5


Yellow 6


UK


48


48


48


48


48


48


Copyright 2019 - Hands-On Labs | http://holscience.com 3 / 5


Sample Spot 1 mm


Blue 1


Blue 2


Red 3


Red 40


Yellow 5


Yellow 6


UK


Sample Spot 1 R


Blue 1


Blue 2


Red 3


Red 40


Yellow 5


Yellow 6


UK


Sample Spot 2 mm


Blue 1


Blue 2


48


32


0


0


1


2


3


1


f


1.5


0


0


48


24


16


48


40


1


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Red 3


Red 40


Yellow 5


Yellow 6


UK


Sample Spot 2 R


Blue 1


Blue 2


Red 3


Red 40


Yellow 5


Yellow 6


UK


Sample Spot 3 mm


Blue 1


Blue 2


Red 3


Red 40


Yellow 5


Yellow 6


-4


3


10


7


3


f


1.2


48


-12


16


4.8


6.86


16


47


2


-6


3


18


8


Copyright 2019 - Hands-On Labs | http://holscience.com 5 / 5


Data Table 2: FD&C Dyes in Sample


UK


Sample Spot 3 R


Blue 1


Blue 2


Red 3


Red 40


Yellow 5


Yellow 6


UK


4


f


1.02


-24


-8


16


2.67


6


12


Sample: FD&C Dyes in Sample


Unknown Sample Red 40


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