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Data table 1 chromatogram data

08/10/2021 Client: muhammad11 Deadline: 2 Day

Four Lab Reports

I would like you to write four lab reports about four different experiments. I will upload you the instructions on how does the report have to be done. Also, I will upload you the experiment file ( this file will contain everything data, question and answers, purpose, etc..) I would like you to rewrite this answers I wrote on your own words. I will also upload you a sample for an old lab report. I want two pages for each experiment eight in total. Thank you!

Mohammed Alnaimi

Student ID 48408 Lesson Chromatography of Food Dyes Institution Ocean County College Session 2019L3 CHEM 182 DL1 Course CHEM 182 DL1 Instructor Nancy Marashi

Final Report

Exercise 1

CHEM 182 DL1 Chromatography of Food Dyes

There will be a separation of com ponents of ink. This could create an error with the com ponents that are trying to separate. This said, a pen could not be used.

1. What might happen if a student used a pen to mark the baseline on the chromatography paper?

The solution will m ove to the top and you would not be able to calculate the Rf value that is com pared to the distance that a com ponent traveled in the stationary phase reached by the solvent.

2. What would happen if the student did not stop the development before the solvent reached the top of the paper?

The solvent would wash away the dye.

3. What would happen if the solvent level was above that of the dye spots on the paper at the start of the development?

Copyright 2019 - Hands-On Labs | http://holscience.com 2 / 5

Photo 1: Chromatogram

Data Table 1: Chromatogram Data

Because the solvents used for chrom atography are volatile. If it is not m arked im m ediately, there is a possibility that it m ay evaporate and then you would not be able to draw the solvent.

4. Why is it important to mark the solvent level on the chromatography paper as soon as you remove it f rom the Petri dish?

Sample Solvent front (mm)

Blue 1

Blue 2

Red 3

Red 40

Yellow 5

Yellow 6

UK

48

48

48

48

48

48

Copyright 2019 - Hands-On Labs | http://holscience.com 3 / 5

Sample Spot 1 mm

Blue 1

Blue 2

Red 3

Red 40

Yellow 5

Yellow 6

UK

Sample Spot 1 R

Blue 1

Blue 2

Red 3

Red 40

Yellow 5

Yellow 6

UK

Sample Spot 2 mm

Blue 1

Blue 2

48

32

0

0

1

2

3

1

f

1.5

0

0

48

24

16

48

40

1

Copyright 2019 - Hands-On Labs | http://holscience.com 4 / 5

Red 3

Red 40

Yellow 5

Yellow 6

UK

Sample Spot 2 R

Blue 1

Blue 2

Red 3

Red 40

Yellow 5

Yellow 6

UK

Sample Spot 3 mm

Blue 1

Blue 2

Red 3

Red 40

Yellow 5

Yellow 6

-4

3

10

7

3

f

1.2

48

-12

16

4.8

6.86

16

47

2

-6

3

18

8

Copyright 2019 - Hands-On Labs | http://holscience.com 5 / 5

Data Table 2: FD&C Dyes in Sample

UK

Sample Spot 3 R

Blue 1

Blue 2

Red 3

Red 40

Yellow 5

Yellow 6

UK

4

f

1.02

-24

-8

16

2.67

6

12

Sample: FD&C Dyes in Sample

Unknown Sample Red 40

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