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Data table 1 microscope components

28/12/2020 Client: saad24vbs Deadline: 3 days

Microscopy for Microbiology - Use and Function


Hands-On Labs, Inc. Version 42-0249-00-02


Lab Report Assistant


This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions, diagrams if needed, and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports by providing this information in an editable file which can be sent to an instructor.


Exercise 1: Getting to Know your Compound Microscope


Data Table 1. Microscope Components.


Letter


Component Name


Component Function


A


B


C


D


E


F


G


H


I


J


K


L


M


Data Table 2. Total Magnification.


Lens


Ocular Magnification


Objective Magnification


Total Magnification


Scanning


Low Power


High Power


Oil Immersion


Data Table 3. Field of View.


Lens


Total Magnification


Field of View (mm)


Field of View (µM)


Scanning


Low Power


High Power


Oil Immersion


Data Table 4. Letter e Viewing Results.


Lens


Photograph


Observations


Scanning


Low


High


Oil Immersion


Questions


A. Describe the details in the slides "Letter e" that become visible as the power changed from scanning power, to low power, to high power.


B. Why is it important to calculate the diameter of the field when first using the microscope?



Exercise 2: Viewing Prepared Microbe Slides


Data Table 5. Prepared Slide Viewing Results.


Slide


Photograph


Total Magnification


Amoeba


Penicillium


Yeast


Spirillium


Bacillus


Coccus


Questions


A. Using the field of view calculated in Exercise 1 for the high power lens, approximately how far across are each of the cells in the Bacteria Coccus Form slide in Data Table 5? Show your calculations.


B. Detail techniques you found helpful for focusing on the various slides in this exercise.


Exercise 3: Preparing Wet-Mount Slides


Data Table 6. Wet-Mount Viewing Results.


Slide


Photograph


Total Magnification


Cheek Cell Smear


Dental Tatar Smear


Questions


A. Describe the similarities and differences between the cheek cell wet mount and dental plaque wet mount.


B. How did the process of preparing wet-mount slides become easier as you prepared the second wet-mount slide of this exercise?


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