Loading...

Messages

Proposals

Stuck in your homework and missing deadline? Get urgent help in $10/Page with 24 hours deadline

Get Urgent Writing Help In Your Essays, Assignments, Homeworks, Dissertation, Thesis Or Coursework & Achieve A+ Grades.

Privacy Guaranteed - 100% Plagiarism Free Writing - Free Turnitin Report - Professional And Experienced Writers - 24/7 Online Support

Effect of ph and temperature on enzyme activity lab report

13/10/2021 Client: muhammad11 Deadline: 2 Day

Lab Report

Title: The Effect of Temperature and pH on Enzyme Activity

OBJECTIVE/INTRODUCTION

The purpose of our lab was to observe how the reaction rate of an enzyme increases or decreases when combined with different substrates. Enzymes are catalysts that increase the rate of biochemical reactions. The rate of these reactions can be affected by its environment: temperature and pH. Low temperatures can cause enzyme reaction rate to become inactive or very slow by causing less collision of molecules and high temperatures can cause enzymes to denature prompting the reaction rate to lessen. As temperature increases, the rate of an enzyme-catalyzed reaction should increase. Similarly, the pH of an environment can affect the shape of an enzyme decreasing or increasing its reaction. In addition, all enzymes have a different optimal pH. As pH increases, the rate of an enzyme-catalyzed reaction should increase. In this lab, to examine the effects of temperature and pH on the reaction rate of an enzyme, we utilized potato extract (catecholase) as our enzyme combined with the substrate catechol. We utilized the Spec 20 to determine the reaction rate of enzymes under different conditions because once the enzyme and substrate are combined in the test tube the reaction begins to occur forming benzoquinone. The product benzoquinone is a brown color and the darker the color the less light will be able to pass through the test tube causing the percentage of light absorbed to increase. The absorption of light will be measured by the Spec 20 allowing us to observe the enzymes reaction rate.

MATERIALS/METHOD

Spectrometer

Pipette

Catechol

Potato Extract

Glass Tubes

This lab was a two-part lab where we analyzed the rate of enzyme reactions at different temperatures and later we looked at how pH levels are affected by enzyme-catalyzed reactions. To test the effects of temperature on enzymatic reactions, we filled 5 test tubes with 4 mL of water and 1 mL of potato extract. Then we filled another tube, which we referred to as the blank, with 1 mL of potato extract and 6ml of water (the extra 2 mL of water is added to offset the 2mL of catecholase that will be added later). From here we put all the tubes except the blank, which served as the control of the experiment, into different temperature environments. The temperatures used were 0℃, 18 ℃, 30 ℃, 40 ℃, and 60 ℃. We left the tubes in these conditions for 5 minutes before adding catechol to the tubes then we reinserted the tubes back in these temperature controlled environments for another 5 minutes. The next step after this was to test light absorbance through the use of the Photospectrometer (Spec 20). The higher the absorbance value determined the rate of the chemical reaction. For the section portion of the lab, we tried to see how pH balance affects enzymatic reaction rates. We filled the 5 tubes with 1 mL of potato extract and 4 mL of pH buffer solution. Each tube had varying pH buffer levels. The levels were pH levels of 3, 5, 7, 9, 11 and of course the blank which is our control tube was filled with 1 mL of potato extract and 4 mL of pH 7 buffer which is neutral. We then used parafilm and inverted to mix each solution and added 2 mL of catechol to all 5 test tubes. We waited 5 minutes for the chemical reaction to take place before calibrating the Spec 20 to test the absorbance.

RESULTS

Table 1: Effect of Temperature on Enzyme Reaction Rate

Sample

Temp (℃)

Absorbance after (5 minutes)

1

0℃

.167

2

18℃

.179

3

30℃

.202

4

40℃

.211

5

60℃

.112

Graph 1: Effect of Temperature on Enzyme Reaction Rate

Table 2: Effect of pH on Enzyme Reaction Rate

Sample

pH

Absorbance after (5 minutes)

1

3

.107

2

5

.121

3

7

.197

4

9

.007

5

11

.010

Graph 2: Effect of pH on Enzyme Reaction Rate

As seen in table and graph 1, the effect of temperature on enzyme reaction rate, as the temperature increased the absorbance also increased until it peaked at sample 4 with a temperature of 40℃ which had an absorbance of .211. In the last sample, sample 5 with a temperature of 60℃, the absorbance began to decrease with an absorbance of .112. In table and graph 2, the effect of pH on enzyme reaction rate, the highly acidic samples (1 and 2) had greater absorbance than the basic samples (4 and 5) which had the lowest absorbance out of all the samples. The highest absorbance was in sample 3 (absorbance of .197) which had a neutral pH of 7.

DISCUSSION (need to be done)

Directions: This section should not just be a restatement of the results but should emphasize interpretation of the data, relating them to existing theory and knowledge. Suggestions for the improvement of techniques or experimental design may also be included here. In writing this section, you should explain the logic that allows you to accept or reject your original hypotheses. Provide a conclusion based on the results that you got.

example: My hypothesis was clearly false, as some ions or cofactors had more rates of absorbency than others. This data suggested that copper is the most crucial to the enzymatic reaction as it had the lowest absorbency when bound to prevent reaction. Cofactors such as copper, calcium, and magnesium were tested by using chelating agents to bind them to prevent them from catalyzing them enzymatic reaction. As a result, their efficiency entirely depended on low absorbance rate. Since Tube 4 was the control, we would have thought that it would have the highest absorbance rate as the enzymatic reaction there were no chelating agents binding it.This may have been a possible error such as miscalculation on the portion of the mixture such as having lest EDTA chelating agents, that Tube 1 had fingerprints.There may also be possible errors such as

inaccurate timing mixing the catechol into the solution or the water bath. If this is not the case, we would suspect that there are cofactors of the enzyme that could actually slow down the enzymatic reaction rather than speeds it up. The rate of absorbance was calculated by subtracting the absorbency at 20 minutes to the absorbency at 10 minutes. I concluded this experiment may have possible errors, but suggests that copper bound by Citric acid is the most effective for the

enzymatic reaction of benzoquinone.

Homework is Completed By:

Writer Writer Name Amount Client Comments & Rating
Instant Homework Helper

ONLINE

Instant Homework Helper

$36

She helped me in last minute in a very reasonable price. She is a lifesaver, I got A+ grade in my homework, I will surely hire her again for my next assignments, Thumbs Up!

Order & Get This Solution Within 3 Hours in $25/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 3 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

Order & Get This Solution Within 6 Hours in $20/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 6 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

Order & Get This Solution Within 12 Hours in $15/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 12 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

6 writers have sent their proposals to do this homework:

A Grade Exams
Engineering Mentor
24/7 Assignment Help
Peter O.
Assignment Hub
Quality Assignments
Writer Writer Name Offer Chat
A Grade Exams

ONLINE

A Grade Exams

You can award me any time as I am ready to start your project curiously. Waiting for your positive response. Thank you!

$25 Chat With Writer
Engineering Mentor

ONLINE

Engineering Mentor

Give me a chance, i will do this with my best efforts

$48 Chat With Writer
24/7 Assignment Help

ONLINE

24/7 Assignment Help

You can award me any time as I am ready to start your project curiously. Waiting for your positive response. Thank you!

$21 Chat With Writer
Peter O.

ONLINE

Peter O.

I have read your project details. I can do this within your deadline.

$40 Chat With Writer
Assignment Hub

ONLINE

Assignment Hub

Give me a chance, i will do this with my best efforts

$39 Chat With Writer
Quality Assignments

ONLINE

Quality Assignments

I have read your project details. I can do this within your deadline.

$26 Chat With Writer

Let our expert academic writers to help you in achieving a+ grades in your homework, assignment, quiz or exam.

Similar Homework Questions

Biology - High level requirements document - Cta code of ethics - Cu h20 6 2 - Ch3oc ch2och3 3 h nmr spectrum - Use a graph to solve each inequality - HW 4 - Steve anthony and persuasion band - Amtel pty ltd perth - NURSING: EVIDENCED-BASED PRACTICE PROJECT - Types of sickle scalers - Informative speech about music - Food safety program records - Week 2 Project Phil - Bismillah food store luton - Br electron configuration full - Sso.bncollege - Khan academy valence electrons - Conflict in romeo and juliet - The miller's tale moral lesson - Household waste disposal sites in hertfordshire - What is a pcbu - A company is considering building a bridge across a river - C++ basic - Demand for Products and Goods - State based action coalitions - Centre of pressure experiment discussion - First national bank charges 10.1 percent compounded monthly - Http www apa org topics kids media food aspx - #14 - Dolch and fry sight words - Royal arch chapter room layout - Week 1 for jessica - Bbc bitesize specialised cells - Narration-Description (Submit Final one-page response to the "Lottery" Essay prompt on the discussion board) Here) - Ratio homework - What do you call a group of factory foreman worksheet - Coca cola process costing system - Does carlos from desperate housewives stay blind - Basic principles of soft switching - Gucci market segmentation - More experience bigger brain rosenzweig - Consultant's analysis report - American government - How to leave hialeah pdf - Succession planning case study nike - Chain of custody flow chart - 3 things im grateful for - The lion the witch and the wardrobe essay - International journal of choice theory and reality therapy - Uber going global from day one - At penn foster expanded academic asap is - Industrial and hazardous waste - BI- project - Rules to write a sonnet - Law & Ethics in the Business Environment - National code of practice for working at heights - Content writing - App - Post - Managerial Epidemiology Week 2 project - The use of absorption costing can result in misleading product cost information. - The Rising Demand for Specialized Tax Consultancy Services - Lab density of solids assignment lab report - Prestige auto parts taren point - Hydrochloric acid and calcium carbonate reaction equation - Project Management - 8 tyee street box hill - How many times does 4 go into 100 - How to find saturation current of diode - Paul's nerang mechanical workshop - Pearson professional centers-parramatta nsw - Ob gyn soap note template - Week8 discussion - Performance with purpose the promise of pepsico - Antarctica project year 6 - What is a discourse community essay - X2 14x 33 0 - One page memo - Performance Management - Section 2.1 frequency distributions and their graphs answers - ME - Online - Research paper 2 - Learning Strategies for e - Malcolm bricklin net worth 2018 - Introduction to Ethics Discussion - Habitable planet ecology lab - Significance of curley's name - Chapter 8 activity 8.1 test your management iq - Healthcare reimbursement - I want a wife judy brady questions - Leading and managing in nursing free pdf - The repetition of initial consonant sounds - Ib schools in miami - Ethical Considerations - Future trends in public health - For every person who has the flu - Kpk police phone numbers - Long service leave provision calculation aasb - Hey brother coca cola lyrics - Sydney gazette war of extermination - The odyssey study guide guided reading questions answers