Loading...

Messages

Proposals

Stuck in your homework and missing deadline? Get urgent help in $10/Page with 24 hours deadline

Get Urgent Writing Help In Your Essays, Assignments, Homeworks, Dissertation, Thesis Or Coursework & Achieve A+ Grades.

Privacy Guaranteed - 100% Plagiarism Free Writing - Free Turnitin Report - Professional And Experienced Writers - 24/7 Online Support

Enzyme ph and temperature lab report

13/10/2021 Client: muhammad11 Deadline: 2 Day

Title: The Effect of Temperature and pH on Enzyme Activity

OBJECTIVE/INTRODUCTION

The purpose of our lab was to observe how the reaction rate of an enzyme increases or decreases when combined with different substrates. Enzymes are catalysts that increase the rate of biochemical reactions. The rate of these reactions can be affected by its environment: temperature and pH. Low temperatures can cause enzyme reaction rate to become inactive or very slow by causing less collision of molecules and high temperatures can cause enzymes to denature prompting the reaction rate to lessen. As temperature increases, the rate of an enzyme-catalyzed reaction should increase. Similarly, the pH of an environment can affect the shape of an enzyme decreasing or increasing its reaction. In addition, all enzymes have a different optimal pH. As pH increases, the rate of an enzyme-catalyzed reaction should increase. In this lab, to examine the effects of temperature and pH on the reaction rate of an enzyme, we utilized potato extract (catecholase) as our enzyme combined with the substrate catechol. We utilized the Spec 20 to determine the reaction rate of enzymes under different conditions because once the enzyme and substrate are combined in the test tube the reaction begins to occur forming benzoquinone. The product benzoquinone is a brown color and the darker the color the less light will be able to pass through the test tube causing the percentage of light absorbed to increase. The absorption of light will be measured by the Spec 20 allowing us to observe the enzymes reaction rate.

MATERIALS/METHOD

Spectrometer

Pipette

Catechol

Potato Extract

Glass Tubes

This lab was a two-part lab where we analyzed the rate of enzyme reactions at different temperatures and later we looked at how pH levels are affected by enzyme-catalyzed reactions. To test the effects of temperature on enzymatic reactions, we filled 5 test tubes with 4 mL of water and 1 mL of potato extract. Then we filled another tube, which we referred to as the blank, with 1 mL of potato extract and 6ml of water (the extra 2 mL of water is added to offset the 2mL of catecholase that will be added later). From here we put all the tubes except the blank, which served as the control of the experiment, into different temperature environments. The temperatures used were 0℃, 18 ℃, 30 ℃, 40 ℃, and 60 ℃. We left the tubes in these conditions for 5 minutes before adding catechol to the tubes then we reinserted the tubes back in these temperature controlled environments for another 5 minutes. The next step after this was to test light absorbance through the use of the Photospectrometer (Spec 20). The higher the absorbance value determined the rate of the chemical reaction. For the section portion of the lab, we tried to see how pH balance affects enzymatic reaction rates. We filled the 5 tubes with 1 mL of potato extract and 4 mL of pH buffer solution. Each tube had varying pH buffer levels. The levels were pH levels of 3, 5, 7, 9, 11 and of course the blank which is our control tube was filled with 1 mL of potato extract and 4 mL of pH 7 buffer which is neutral. We then used parafilm and inverted to mix each solution and added 2 mL of catechol to all 5 test tubes. We waited 5 minutes for the chemical reaction to take place before calibrating the Spec 20 to test the absorbance.

RESULTS

Table 1: Effect of Temperature on Enzyme Reaction Rate

Sample

Temp (℃)

Absorbance after (5 minutes)

1

0℃

.167

2

18℃

.179

3

30℃

.202

4

40℃

.211

5

60℃

.112

Graph 1: Effect of Temperature on Enzyme Reaction Rate

Table 2: Effect of pH on Enzyme Reaction Rate

Sample

pH

Absorbance after (5 minutes)

1

3

.107

2

5

.121

3

7

.197

4

9

.007

5

11

.010

Graph 2: Effect of pH on Enzyme Reaction Rate

As seen in table and graph 1, the effect of temperature on enzyme reaction rate, as the temperature increased the absorbance also increased until it peaked at sample 4 with a temperature of 40℃ which had an absorbance of .211. In the last sample, sample 5 with a temperature of 60℃, the absorbance began to decrease with an absorbance of .112. In table and graph 2, the effect of pH on enzyme reaction rate, the highly acidic samples (1 and 2) had greater absorbance than the basic samples (4 and 5) which had the lowest absorbance out of all the samples. The highest absorbance was in sample 3 (absorbance of .197) which had a neutral pH of 7.

DISCUSSION (need to be done)

Directions: This section should not just be a restatement of the results but should emphasize interpretation of the data, relating them to existing theory and knowledge. Suggestions for the improvement of techniques or experimental design may also be included here. In writing this section, you should explain the logic that allows you to accept or reject your original hypotheses. Provide a conclusion based on the results that you got.

example: My hypothesis was clearly false, as some ions or cofactors had more rates of absorbency than others. This data suggested that copper is the most crucial to the enzymatic reaction as it had the lowest absorbency when bound to prevent reaction. Cofactors such as copper, calcium, and magnesium were tested by using chelating agents to bind them to prevent them from catalyzing them enzymatic reaction. As a result, their efficiency entirely depended on low absorbance rate. Since Tube 4 was the control, we would have thought that it would have the highest absorbance rate as the enzymatic reaction there were no chelating agents binding it.This may have been a possible error such as miscalculation on the portion of the mixture such as having lest EDTA chelating agents, that Tube 1 had fingerprints.There may also be possible errors such as

inaccurate timing mixing the catechol into the solution or the water bath. If this is not the case, we would suspect that there are cofactors of the enzyme that could actually slow down the enzymatic reaction rather than speeds it up. The rate of absorbance was calculated by subtracting the absorbency at 20 minutes to the absorbency at 10 minutes. I concluded this experiment may have possible errors, but suggests that copper bound by Citric acid is the most effective for the

enzymatic reaction of benzoquinone.

Homework is Completed By:

Writer Writer Name Amount Client Comments & Rating
Instant Homework Helper

ONLINE

Instant Homework Helper

$36

She helped me in last minute in a very reasonable price. She is a lifesaver, I got A+ grade in my homework, I will surely hire her again for my next assignments, Thumbs Up!

Order & Get This Solution Within 3 Hours in $25/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 3 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

Order & Get This Solution Within 6 Hours in $20/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 6 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

Order & Get This Solution Within 12 Hours in $15/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 12 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

6 writers have sent their proposals to do this homework:

Custom Coursework Service
Assignment Guru
Instant Homework Helper
Financial Solutions Provider
Coursework Help Online
Homework Tutor
Writer Writer Name Offer Chat
Custom Coursework Service

ONLINE

Custom Coursework Service

I have read and understood all your initial requirements, and I am very professional in this task.

$34 Chat With Writer
Assignment Guru

ONLINE

Assignment Guru

I will cover all the points which you have mentioned in your project details.

$30 Chat With Writer
Instant Homework Helper

ONLINE

Instant Homework Helper

Hello, I an ranked top 10 freelancers in academic and contents writing. I can write and updated your personal statement with great quality and free of plagiarism

$24 Chat With Writer
Financial Solutions Provider

ONLINE

Financial Solutions Provider

I have read and understood all your initial requirements, and I am very professional in this task.

$47 Chat With Writer
Coursework Help Online

ONLINE

Coursework Help Online

I have read and understood all your initial requirements, and I am very professional in this task.

$19 Chat With Writer
Homework Tutor

ONLINE

Homework Tutor

I have read and understood all your initial requirements, and I am very professional in this task.

$36 Chat With Writer

Let our expert academic writers to help you in achieving a+ grades in your homework, assignment, quiz or exam.

Similar Homework Questions

Discussion 6 - Christian counseling code of ethics - Glow stick liquid experiment - 5 paragraph discussion - Tando stone bucks county gray - For my own good all causes shall give way - Market entry modes for international businesses chapter 7 - 2 syllable words with double consonants - Yellow worksheet accounting - Homework - Objective of front office department - Determination of acetic acid in commercial vinegar using naoh - Principle of lateral continuity geology - Which landform is part of the cryosphere - How to calculate usual body weight - Fnb non resident account requirements - Paula Plaintiff's - Cisco eem tcl script examples - HA575 Assignment 2 - Chapter 5 medicine and law study guide answers - 753 rowland road fairfield ct - Sheffield city council job vacancies - Energy dissipation in hydraulic jump - Business Paper - Fired heaters in refineries - Week 4 6640 - Piercemil instructure - Yearbook of labour statistics - Dana corporation toledo ohio - JCCMI- Week 6 Discussion 1 and 2 - Everybody from the 313 beat - Evidence for evolution powerpoint notes answer key - Taxation (120 words) - Fitness fanatics is a regional chain of health - Excel - Mullineaux corporation has a target capital structure - Strong thesis statement about technology - Big data means big potential challenges for nurse execs - Holes louis sachar quiz - If then else sas - Death investigation week1 - Soap Note : " Prostate Cancer " - Weekly tax table casual - How to write a balanced sentence - Starbucks strategic management process model - A beautiful mind movie questions answers - Cambridge latin course book 2 translations stage 16 - A german worker takes 400 hours - How is long service leave calculated in sa - Computer software lesson plans - Intoto kitchens northwood hills - Measuring distance in space worksheet - The finite population correction factor is used when - Diarmuid smyth sword security - Deing v tarola 1993 - Audit tender document acca - GLOB (U4_RPLx2) - Food Labels! - Reducing COPD readmissions in those aged 75-80; legislation/policy 30-day readmission policy - Inlanguage boutique brunswick east - RN Capstone Week 5 - Developmental matrix - Bonaire ducted heating warranty - Hicaps add provider form - 3 bats in the belfry lodi wi - Umuc haircuts - Novex sharp prestained protein standard 57318 - Help 1 - Module 05 Written Assignment - Sexually Transmitted Infections - Brooklands vip pet products - 2013 pearson education inc answers - Why do you want to work at trader joe's answer - Code of Ethics Intake Packet - Cap 1 M5 - Conni critters pet pad - Sanger sequencing mcgraw hill - Quantitative Methods - Coca cola csr india - Nosql and sql data modeling ted hills pdf - Death by scrabble theme - BA Plan Capital Water Expert - Essay - Bit ly 247careers - A _______ is often used to cut holes to install outlet boxes in old installations. - Global supply chain game - Econ test - Chapter 12 review biology answers - Miriam rose ungunmerr baumann biography - The book thief part 7 - Waves on a string phet lab answers - Operation anaconda battle analysis - Spelt risotto trader joe's discontinued - Research Paper - Product count capsim - Creating an activity series of metals lab - Blind and buried vias altium - Managerial accounting final exam solutions - Fit out cost per square metre dubai - Gas insulated switchgear definition - Is nick carraway a reliable narrator essay