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Lab 2 Introduction to Culturing & Aseptic Technique “BIO250L”
Student Name: Click here to enter text.
Access Code (located on the lid of your lab kit): Click here to enter text.
“Experiment 1- Agar Plate Preparation and Bacterial Inoculation”
“Table 1: Experiment 1 Colony Growth”
“Plate #”
“Condition/Surface”
“Growth (color, amount, shape, etc.)”
“1”
Small and smooth growth from human mouth
Yellow, small, circular
“2”
Glistening growth from bathroom tub
Large, milky and thin
“3”
Growth from cell phone
Yellow, medium and circular
“4”
Air for 1 hour
Small, white and buffy like
“5”
Control plate
No growth
“Post-Lab Questions”
“1. Which plate grew the most bacterial species (based on number of different colonies)? Was this a surprise? Why or why not?”
The bathroom tub registered the largest number of bacterial growth. This was not a surprise as the bathroom tub was not disinfected properly.
“2. Was the control plate free of bacterial colonies? If yes, what does this mean for your experiment? If not, what could be the source of the contamination?”
Yes, the control plate did not have any bacterial growth. This implies that proper aseptic techniques was followed during preparation of the plate
“3. Did the airborne contamination plate contain any growth? Were any of the colonies similar in appearance to those found on the other plates? How does this plate demonstrate the need for following aseptic technique when culturing bacteria?”
Yes, the air borne plate contained molds. The colonies were different from other plates. Aseptic techniques ensures that no contamination occurs which means even molds should not grow.
“Insert photo of your cultures after incubation with your name clearly visible in the background:”
Drag and drop your photo here:
“Experiment 2- Bacterial Transfer to a Stab Tube and an Agar Plate”
“Table 2: Initial Reserved Plate Colony Growth Observations”
“Colony”
“Plate Sample”
“Appearance (color, morphology, etc.)”
“1”
Phone
Black and jagged
“2”
Shoe
Yellow and circular
“3”
Mouth
Green and jagged
“Table 3: Final Plate and Stab Tube Growth Observations”
“Sample”
“Form”
“Growth?”
“Same Appearance as Initial Plate?”
“Effective Transfer?”
“1”
“Plate”
No
No
No
“Stab Tube”
Yes
No
No
“2”
“Plate”
Yes
Yes
Yes
“Stab Tube”
Yes
Yes
Yes
“3”
“Plate”
Yes
Yes
Yes
“Stab Tube”
Yes
Yes
Yes
“Control”
“Plate”
No
N/A
N/A
“Stab Tube”
No
N/A
N/A
“Post-Lab Questions”
“1. Did the colonies that grew on the transfer plates retain the same appearance from the initial plate? Why or why not?”
Yes, the colonies retained the same appearance. This is because of successful aseptic transfer.
“2. Were the colonies uniform in appearance on the transfer? What could account for any differences seen?”
The colonies were not uniform. This may be due to differences in nutrient content
“3. Did growth occur through the depth of the stab tube? If not, what could account for the lack of bacterial growth?”
No. Growth did not occur due to anerobic conditions
“4. Why was it important to include a control plate and stab tube in this experiment? Did they serve as positive or negative controls?”
To compare the control plate with other plates to ascertain growth. They served as negative controls.
“Insert photo of your cultures after incubation with your name clearly visible in the background:”
Drag and drop your photo here:
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