Loading...

Messages

Proposals

Stuck in your homework and missing deadline? Get urgent help in $10/Page with 24 hours deadline

Get Urgent Writing Help In Your Essays, Assignments, Homeworks, Dissertation, Thesis Or Coursework & Achieve A+ Grades.

Privacy Guaranteed - 100% Plagiarism Free Writing - Free Turnitin Report - Professional And Experienced Writers - 24/7 Online Support

Experiment 2: diffusion - concentration gradients and membrane permeability

31/10/2020 Client: papadok01 Deadline: 7 Days

image3.png

Experiment 2: Concentration Gradients and Membrane Permeability

In this experiment, you will dialyze a solution of glucose and starch to observe:

· The directional movement of glucose and starch.

· The effect of a selectively permeable membrane on the diffusion of these molecules.

An indicator is a substance that changes color when in the presence of a specific substance. In this experiment, IKI will be used as an indicator to test for the presence of starch.

Materials

(5) 100 mL Beakers 10 mL 1% Glucose Solution, C6H12O6 4 Glucose Test Strips (1) 100 mL Graduated Cylinder 4 mL 1% Iodine-Potassium Iodide, IKI 5 mL Liquid Starch, C6H10O5 3 Pipettes 4 Rubber Bands (Small; contain latex, handle with gloves on if allergic)

Permanent Marker *Stopwatch *Water *Scissors

*15.0 cm Dialysis Tubing *You Must Provide *Be sure to measure and cut only the length you need for this experiment. Reserve the remainder for later experiments.

image3.png

Attention!

Do not allow the open end of the dialysis tubing to fall into the beaker. If it does, remove the tube and rinse thoroughly with water before refilling it with the starch/glucose solution and replacing it in the beaker.

Note:

If you make a mistake, the dialysis tubing can be rinsed and used again.

Dialysis tubing must be soaked in water before you will be able to open it up to create the dialysis “bag.” Follow these directions for this experiment:

1. Soak the tubing in a beaker of water for ten minutes.

2. Place the dialysis tubing between your thumb and forefinger, and rub the two digits together in a shearing manner. This motion should open up the “tube” so that you can fill it with the different solutions.

Procedure

1. Measure and pour 50 mL of water into a 100 mL beaker using the 100 mL graduated cylinder. Cut a piece of dialysis tubing 15.0 cm long. Submerge the dialysis tubing in the water for at least ten minutes.

2. Measure and pour 82 mL of water into a second 100 mL beaker using the 100 mL graduated cylinder. This is the beaker you will put the filled dialysis bag into in Step 9.

3. Make the glucose/sucrose mixture. Use a graduated pipette to add 5 mL of glucose solution to a third 100 mL beaker and label it “dialysis bag solution.” Use a different graduated pipette to add 5 mL of starch solution to the same beaker. Mix by pipetting the solution up and down six times.

4. Using the same pipette that you used to mix the dialysis bag solution, remove 2 mL of the dialysis bag solution and place it in a clean beaker. This sample will serve as your positive control for glucose and starch.

a. Dip one of the glucose test strips into the 2 mL of glucose/starch solution in the third beaker. After one minute has passed, record the final color of the glucose test strip in Table 3. This is your positive control for glucose.

b. Use a pipette to transfer approximately 0.5 mL of IKI into the 2 mL of glucose/starch solution into the third beaker. After one minute has passed, record the final color of the glucose/starch solution in the beaker in Table 3. This is your positive control for starch.

5. Using a clean pipette, remove 2 mL of water from the 82 mL of water you placed in a beaker in Step 2, and place it in a clean beaker. This sample will serve as your negative controls for glucose and starch.

a. Dip one of the glucose test strips into the 2 mL of water in the beaker. After one minute has passed, record the final color of the glucose test strip in Table 3. This is your negative control for glucose.

b. Use a pipette to transfer approximately 0.5 mL of IKI into the 2 mL in the beaker. After one minute has passed, record the final color of the water in the beaker in Table 3. This is your negative control for starch.

Note: The color results of these controls determine the indicator reagent key. You must use these results to interpret the rest of your results.

6. After at least ten minutes have passed, remove the dialysis tube and close one end by folding over 3.0 cm of one end (bottom). Fold it again and secure with a rubber band (use two rubber bands if necessary).

7. Test to make sure the closed end of the dialysis tube will not allow solution to leak out. Dry off the outside of the dialysis tube bag with a cloth or paper towel. Then, add a small amount of water to the bag and examine the rubber band seal for leakage. Be sure to remove the water from the inside of the bag before continuing.

8. Using the same pipette that was used to mix the solution in Step 3, transfer 8 mL of the dialysis bag solution to the prepared dialysis bag.

image2.jpg

Figure 4: Step 9 reference.

9. Place the filled dialysis bag in the 100 mL beaker filled with 80 mL of water, leaving the open end draped over the edge of the beaker as shown in Figure 4.

10. Allow the solution to sit for 60 minutes. Clean and dry all materials except the beaker holding the dialysis bag.

11. After the solution has diffused for 60 minutes, remove the dialysis bag from the beaker and empty the contents of the bag into a clean, dry beaker. Label the beaker “final dialysis bag solution.”

12. Test the final dialysis bag solution for the presence of glucose by dipping one glucose test strip into the dialysis bag. Wait one minute before reading the results of the test strip. Record your results for the presence of glucose in Table 4.

13. Test for the presence of starch by adding 2 mL IKI. After one minute has passed, record the final color in Table 4.

14. Use a pipette to transfer 8 mL of the water in the beaker to a clean beaker. Test the beaker water for the presence of glucose by dipping one glucose test strip into the beaker. Wait one minute before reading the results of the test strip, and record the results in Table 4.

15. Test for the presence of starch by adding 2 mL of IKI to the beaker water. Record the final color of the beaker solution in Table 4.

Table 3: Indicator Reagent Data

Indicator

Starch Positive Control (Color)

Starch Negative Control (Color)

Glucose Positive Control (Color)

Glucose Negative Control (Color)

Glucose Test Strip

n/a

n/a

IKI Solution

n/a

n/a

Table 4: Diffusion of Starch and Glucose Over Time

Indicator

Dialysis Bag After 60 Minutes

Beaker Water After 60 Minutes

IKI Solution

Glucose Test Strip

Post-Lab Questions

1. Why is it necessary to have positive and negative controls in this experiment?

2. Draw a diagram of the experimental set-up. Use arrows to depict the movement of each substance in the dialysis bag and the beaker.

3. Which substance(s) crossed the dialysis membrane? Support your response with data-based evidence.

4. Which molecules remained inside of the dialysis bag?

5. Did all of the molecules diffuse out of the bag into the beaker? Why or why not?

©eScience Labs, 2016

image4.png

image4.png image5.jpg

Homework is Completed By:

Writer Writer Name Amount Client Comments & Rating
Instant Homework Helper

ONLINE

Instant Homework Helper

$36

She helped me in last minute in a very reasonable price. She is a lifesaver, I got A+ grade in my homework, I will surely hire her again for my next assignments, Thumbs Up!

Order & Get This Solution Within 3 Hours in $25/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 3 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

Order & Get This Solution Within 6 Hours in $20/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 6 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

Order & Get This Solution Within 12 Hours in $15/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 12 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

6 writers have sent their proposals to do this homework:

Top Writing Guru
Essay Writing Help
Online Assignment Help
Financial Hub
Quality Homework Helper
Buy Coursework Help
Writer Writer Name Offer Chat
Top Writing Guru

ONLINE

Top Writing Guru

I am an Academic writer with 10 years of experience. As an Academic writer, my aim is to generate unique content without Plagiarism as per the client’s requirements.

$60 Chat With Writer
Essay Writing Help

ONLINE

Essay Writing Help

I am a qualified and experienced Writer, Researcher, Tutor, analyst and Consultant. I hold MBA (Strategic Management) (Finance and Marketing) & CPA.K (Accounting and Finance.)

$62 Chat With Writer
Online Assignment Help

ONLINE

Online Assignment Help

Hi dear, I am ready to do your homework in a reasonable price.

$55 Chat With Writer
Financial Hub

ONLINE

Financial Hub

Hey, I have gone through your job posting and become very much interested in working with you.I can deliver professional content as per your requirements. I am a multi-skilled person with sound proficiency in the English language for being a native writer who worked on several similar projects of content writing and can deliver quality content to tight deadlines. I am available for both online and offline writing jobs with the promise of offering an incredibly responsive and supreme level of customer service. Thanks!

$55 Chat With Writer
Quality Homework Helper

ONLINE

Quality Homework Helper

Hi dear, I am ready to do your homework in a reasonable price.

$62 Chat With Writer
Buy Coursework Help

ONLINE

Buy Coursework Help

Hi dear, I am ready to do your homework in a reasonable price.

$62 Chat With Writer

Let our expert academic writers to help you in achieving a+ grades in your homework, assignment, quiz or exam.

Similar Homework Questions

Bob beck ozone generator - Discussion Question (AG) - Capella clinical mental health counseling - Romeo and juliet act 5 test - Dorothea orem self care theory - Australian gas networks limited - Simulation exercise - Gut directed hypnotherapy sydney - International journal of trend in scientific research and development ijtsrd - Data analysis is usually guided by - Urgent - Introduction to landscape - Algebra 2 margin of error worksheet - What is a topical essay - 350 word discussion post soc350 - Strategic Management Assignment - Cdisc lab controlled terminology - Help - The mountaintop by katori hall free pdf - Acts 2 1 13 - Mattel Confronts Its Marketing Challenges” - Does bianca die in othello - How does listing effect the reader - Nacl lewis dot structure - Benefits of office automation system - How to find avc average variable cost - 17/7 as a mixed number - Positive guidance philosophy - Directions to holy cross hospital - Which of the following characteristics distinguishes moral standards from other sorts of standards? - 200words - Controls and variables part 2 worksheet answers - Investigation Considerations - Click and clone mimi the mouse answer key - Ngai tahu family tree - Internet bandwidth calculator per user - Navajos wear nikes chapter summaries - In a suit against ian, jenna obtains an injunction. this is - Windshield survey form - A sky full of stars m4a - Non chronological report vocabulary - How to win practice marketing game - Electronic trap primer valve - How to describe a solution - An ethical dilemma relating to global diversity. - Database functions - Tata nano market segmentation - The manager of the danvers hilton resort hotel stated - Elizabethan views on women - Reword Survey - Is volume area x height - The diamond necklace climax - 20 advantages and disadvantages of diversity in the workplace - Under what type of marketing does kliptech operate - Neolithic societies and myths - How would one define business intelligence (B1)? Identify and briefly discuss a real-world application of B1How would one define business intelligence (B1)? Identify and briefly discuss a real-world application of B1 - Booster pump selection guide - Dis 4 - Rationale for nursing interventions - Importance of preventive maintenance ppt - Elisa standard curve prism - Swot and tows analysis pdf - C06 Online Exam 1_02 SCORE 100 PERCENT - Does adam calhoun own grunt style - Retrenching to a narrower diversification base - Auditing - To many the word management suggests 1 - Str 581 capstone final exam part 3 - Sql data analyst resume - Explain various philosophies of marketing - Case study on death and dying grand canyon university - Guy code vs girl code watch online - Why does aguinaldo think that the united states is betraying its own values? - Manage diversity in the workplace bsbdiv501 - Designing a drone for power line inspection - Discussion - Low profile zip ties - Tertiary student concession card nsw - Oakdene care home sleaford - Nmc reflective discussion form - Cage analysis example - Case study on business miscommunication - Cjt102 discussion - Write balanced equation for reaction between na2o2 and water - Write a 3-5 full pages Double Spaced Essay for Africana studies - A 20 kg loudspeaker is suspended 2.0m below - Lintrose nominees pl v king - Module 4 Journal Article Analysis - Accountants must abide by a strict code of ethics that defines their responsibilities to - Commonwealth merchant phone number - Csa ccm v3 0.1 - Master lock engraving form - Chay - Online - Dis 2 - Something wicked this way comes quote - Critical path method solved examples - 2-3 pages summary - Where did marc newson study - Blum minipress p manual - Draftkings something went wrong vpn - Marketing strategy and consumer behavior - Paper 1