Experiment 2 tracking chromosomal dna movement through mitosis

Experiment 1: Following Chromosomal DNA Movement through Meiosis

In this experiment, you will model the movement of the chromosomes through meiosis I and II to create gametes.

Materials

6 Long Pipe Cleaners

4 Beads

*Scissors

*You must provide.

Procedure:

Part 1: Modeling Meiosis without Crossing Over

As prophase I begins, the replicated chromosomes coil and condense…

Build a pair of replicated, homologous chromosomes (Figure 3). One pipe cleaners should be used to create each individual sister chromatid (2 pipe cleaners per chromosome pair). One bead represents each centromere. To do this...
Figure 3: Pipe cleaner set-up. The red pipe cleaners represent one pair of sister chromatids and the orange pipe cleaners represent a second pair of sister chromatids. The red and orange pair are homologous.

Start with two pipe cleaners of the same color to create your first sister chromatid pair. Hold the pipe cleaners next to each other and string the pipe cleaners through one bead. Slide the bead halfway down the pipe cleaners. Then, pull bottom ends of the pipe cleaners away from each other to create an "X" shape.
Repeat this process using the other two matching pipe cleaners to create the second sister chromatid pair.
Cut the two remaining pipe cleaners in half. Repeat step 1 using the two pipe cleaner halves of the same color to create homologous chromosomes (Figure 4).
Pair up the homologous chromosome pairs created in Step 1 and 2. DO NOT SIMULATE CROSSING OVER IN THIS TRIAL. You will simulate crossing over in Part 2.
Configure the chromosomes as they would appear in each of the stages of meiotic division (prophase I and II, metaphase I and II, anaphase I and II, telophase I and II, and cytokinesis).
Diagram the corresponding images for each stage in the sections titled "Trial 1 - Meiotic Division Beads Diagram." Be sure to indicate the number of chromosomes present in each cell for each phase.