Stuck in your homework and missing deadline?

Get Urgent Writing Help In Your Essays, Assignments, Homeworks, Dissertation, Thesis Or Coursework & Achieve A+ Grades.

Privacy Guaranteed - 100% Plagiarism Free Writing - Free Turnitin Report - Professional And Experienced Writers - 24/7 Online Support

Gram staining introduction lab report

01/01/2021 Client: saad24vbs Deadline: 14 Days

Y:\ESL Kits\Manual Graphics\PlainHdr.jpg

Structure and Microscopy

Lab 4: Structure and Microscopy (100 points)

Student Name:

Student ID:

Course ID:

-Each question on the lab worksheet must be answered completely, thoroughly, in complete sentences and correctly in order to be considered for full credit

-If the question asks you to do research or find a source, a reputable, credible and/or scholarly source citation must be included in order to be considered for full credit

-If a math formula is required to arrive to an answer, work must be shown otherwise, no credit will be awarded

Pre-Lab Questions

1. What determines if a bacterial cell is Gram-positive or Gram-negative? (5 points)

Amount and location of the peptidoglycan molecule in the prokaryotic cell wall determines whether a bacterial cell is Gram-positive or Gram-negative.

2. In this lab, both viruses and prions were introduced as acellular organisms. Do some research and describe one other type of acellular organism. What characteristics about this organism classify it as acellular? (5 points)

Viroids are another type of acellular organism along with viruses and prions. They are plant pathogens, which consist only of a short strand of circular RNA capable of self-replication.

3. Bacteria have many different shapes that often determine their class. Research and form a hypothesis on the evolutionary reasons for so many different bacterial morphologies. (5 points)

Each bacterial morphology may be a selectable feature to aid survival and may have affected by different physical, environmental, and biological forces to contribute to natural selection.


Young, K. D. (2006, September). The Selective Value of Bacterial Shape. Retrieved September 30, 2018, from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1594593/

4. Do a search online or look in your textbook for 1-2 antibiotics that affect Gram-positive bacteria and list them. On what part of the cell do the antibiotics usually work? List one or two antibiotics that affect Gram-negative bacteria? On what part of the cell do the antibiotics usually work? (Be sure to cite your sources in your answer.) (5 points)

5. Why do you think it is important to identify a bacterial disease in a patient before prescribing any antibiotic treatments? (Be specific.) (5 points)


Experiment 1 Results Tables

Table 1: Experiment 1 Staining Observations (5 points)

Stain used:

Crystal Violet


Purple rod-shape bacteria with white background were observed

Experiment 1 Post-Lab Questions

1. How does crystal violet enhance the visualization of microbial features? (5 points)

Crystal violet enhances the contrast between the microorganism itself and the slide, making the bacteria appear as purple.

2. What are some of the limitations of simple staining? (5 points)

3. Give an example of a situation in a lab or medical setting in which simple staining would be utilized. (5 points)

Simple staining is used to obtain basic information about morphology of one type of microorganism through clear visualization.

Experiment 2 Results Tables

Table 2: Experiment 2 Staining Observations (5 points)

Stain used:



Background is stained, bacteria shows up as clear spiral.

Experiment 2 Post-Lab Questions

1. After visualizing the stained samples either using your microscope or by looking at the sample images provided, describe what physical/visual characteristics you were able to observe after performing the negative staining vs. after performing the simple stain. (5 points)

After looking at the sample images provided, negatively stained bacteria showed up as clear straight spirals against a dark background. Bacteria that are simple stained showed up as dark purple rods-shaped with white background.

2. So far in this lab, you have used one type of simple stain and one type of negative stain, yet there are many other simple and negative dyes available. Pick one simple dye and one negative dye, and discuss how those dyes differ from the ones you used in this lab. Give a scenario in which their use would be appropriate. (5 points)

Methylene blue is another dye that can be used for negative stain.

India Ink is another type of negative stain.

Experiment 3 Results Tables

Table 3: Experiment 3 Staining Observations (5 points)

Stain used:

Crystal violet (primary stain) & Safranin (counterstain)


Gram-positive appeared as purple and Gram-negative showed up as pink.

Experiment 3 Post-Lab Questions

1. What color are the Gram-positive bacteria after Gram staining? Gram-negative bacteria? (5 points)

Gram-positive bacteria appear as dark purple or blue due to retaining the primary dye (Crystal Violet) in the cell wall.

Gram-negative bacteria appear as red or pink due to decolorizing to accept the counterstain (Safranin).

2. What different characteristic(s) exist between the two groups that account for the different staining conditions? (5 points)

Gram-positive bacteria are stained purple, and gram-negative bacteria stain as pink. They are two distinct morphological groups of bacteria.

3. Why was the Gram iodine added to the Gram staining procedure? (5 points)

Gram iodine is added as a mordant to stabilize the crystal violet iodine complex so that the dye cannot be removed easily.

4. Why is a counterstain (safranin) added to the Gram staining procedure? (5 points)

A counterstain is used to help identify gram-negative bacteria. Gram-negative bacteria lose the crystal violet and stain red.

5. What are the advantages of performing a Gram stain vs. a simple stain for visualizing bacteria? (5 points)

Gram stain contains two or more different stains and can differentiate the species of bacteria into two main groups (gram-positive and gram-negative) by looking at the color of cells (pink or purple). Simple stain involves single stain and it is used to easily determine cell shape, size, and arrangement.

6. Using either a textbook or a reputable online resource, research some of the typical characteristics of bacteria, and discuss why it might be important for a researcher or a hospital technician to be able to differentiate between Gram-positive and Gram-negative bacteria. (5 points)

7. Did you experience any technical difficulties or atypical results during this experiment? If so, what happened, and how could you avoid these issues in the future? (5 points)

Homework is Completed By:

Writer Writer Name Amount Client Comments & Rating
Instant Homework Helper


Instant Homework Helper


She helped me in last minute in a very reasonable price. She is a lifesaver, I got A+ grade in my homework, I will surely hire her again for my next assignments, Thumbs Up!

Guarantee Your Academic Success!

Hire Professional Writers And Get Best Homework Writing Services In Any Subject

6 writers have sent their proposals to do this homework:

Helping Hand
Top Essay Tutor
University Coursework Help
Writer Writer Name Offer Chat
Helping Hand


Helping Hand

I am an Academic writer with 10 years of experience. As an Academic writer, my aim is to generate unique content without Plagiarism as per the client’s requirements.

$230 Chat With Writer
Top Essay Tutor


Top Essay Tutor

I have more than 12 years of experience in managing online classes, exams, and quizzes on different websites like; Connect, McGraw-Hill, and Blackboard. I always provide a guarantee to my clients for their grades.

$235 Chat With Writer
University Coursework Help


University Coursework Help

Hi dear, I am ready to do your homework in a reasonable price.

$232 Chat With Writer

Let our expert academic writers to help you in achieving a+ grades in your homework, assignment, quiz or exam.

Similar Homework Questions

Ladybug daycare lincoln and jersey - Hamartia in a sentence - Basic advice on first aid at work government leaflet - Positive communication climate examples - Weeek-4 - Special cookies and cream polvoron recipe - Modified duration of zero coupon bond - Target corporation case study analysis - Developing a health advocacy campaign walden - Ystwyth veterinary practice ltd - Mountain view realty excel assignment answers - Mythbusters ice bullet - Aston uni wass appointment - Spanish explorer costume ideas - Pats control function module - The transactional model of communication supports - Hobby lobby jobs hays ks - Process design matrix ops 571 - Diseases and peculiarities of the negro race - Incident and reflected waves in transmission lines - Top 5 bicep exercises for the peak - Oracle 12c sql chapter 5 hands on assignments answers - Biology - Business case versus business plan - When was glass windows invented - Usca wellness center hours - Hana cloud integration pdf - How does salt water affect gummy bears - Season of life jeffrey marx sparknotes - I need 2300 words in Project Management Basics to answers my questions - Systemverilog assertions examples with answers - Managing Operations - Peregrine exam criminal justice - I have read and acknowledge example - Cost of Capital - Hartford research issues bonds dated january 1 - Comparison Analysis Essay - Explain the freestyle machine that coke developed - York st john resits - Profession - Measurement of emf of a cell using potentiometer - What variables influence a demand for a normal good - Chivalrous knight crossword clue - Week 6. - Kids shoe size 31 conversion - Walt whitman leaves of grass the notebook - Gap big data case study - Microsoft wireless mouse 5000 - When the fed conducts open market sales - Sleeping giant sons of thunder jacket - Pre employment checks nsw health - Tina jones musculoskeletal objective data - Band booster class java - Krapp last tape summary sparknotes - Intelligence and homeland security - Kitchen utensils and equipment and their uses - Linear piecewise defined functions edgenuity answers - U store it tonsley - Year 5 maths curriculum victoria - Roman jakobson on linguistic aspects of translation pdf - Ratios and financial planning at east coast yachts solution - Judaism places of worship - Chronic pain - Hang on sloopy dancer - Project 4 - Ashworth college intro to computers assignment 8 - Domestic violence sociology a level - Lord i need u by chris tomlin - Www linkedin com checkpoint lg login submit - Credit sales with tax journal entry - Purpose of monitoring inspections - 3 times table rhyme - Common source amplifier at high frequency - How to write a fraction story - The bear by anton chekhov questions - Suttle and king pyramid scheme - Discussion 2 - Alliance outdoor lighting prices - Team rocket kekeke pokemon go - The mean annual cost of automobile insurance is 939 - 100 blackney road the gurdies - Who is the surveyor in the custom house - Columbus sex slave and gold mine - Unit VII Essay - Maths - Business government and society pdf - Amp gauge wiring diagram - Break even sales under present and proposed conditions - Risk Assessment SLP - Hey whipple squeeze this chapter 2 summary - Bluebeam sign document greyed out - Business - Quick start guide call of cthulhu - Traffic warden state management unit midland wa 6056 - Paranoid personality disorder case study examples - Flip chip packaging process - Dr sean tan crows nest - Nick gereffi cause of death you - Willy wonka fat kid - Fuse board regulations ireland