How to prepare an onion slide

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BIO A & EI*BIO APlantCells & Animal Cells under the Microscope Lab # Objective:Observe animal cells and plant cells under the microscope. Prepare wet mount-slides. Describe the differences and similarities between plant cells and animal cells. MATERIALS: Microscope, prepared slides, microscope slides, cover slips, tap water, onions, lettuce, droppers PROCEDURE Prepared slides (3 slides). 1.Observe the prepared slide under the 4X objective. 2.Draw a circle with the Petri-dish and draw in it what you see under the microscope. 3.Label your pictures with names, overall magnification, and any structures you see.4.Do steps 1-3 using the 10X objective. Wet-mount slidesof Plant cells(2 slides) .1.With a slightly twisting motion, tear the onion or/and lettuce leaf. 2.Snip off a small piece of the very thin, transparent tissue that appears on one of the torn edgesof the vegetable. 3.Place this skin on a slide. 4.Add a drop of water. 5.Place a cover slip over the drop being careful that no bubbles form between the slide and the cover slip. 6.Examine with the 4X objective. 7.Draw a circle with a Petri-dish and draw in it what you see under the microscope. 8.Label your pictures with names,overall magnification, and any structures you see.9.Repeat steps 6-8 using the10X objective. DATA (2 drawings for each slide observed!) Name ______________________ 40XName _____________________ 400XCopy this table and indicate with a check mark whether each type of cell has the following organelles LettuceOnionAnimal Cell (the one that you observed) ChloroplastCell wall NucleusCell MembraneMitochondria
BIO A & EI*BIO AANALYSISPrepared slides 1.Did you see individual cells? How could you tell? 2.Were there more than one kind of cells in any one picture? 3.Describe the structures you saw within the cell. Describe the functions of the structures you observed. Wet-mount Slides 4.Why observe the skin of the onion (thin piece) and not an entire thick piece? 5.Describe what you saw in onion cells. 6.What makes an onion a good material to observe? 7.What are some other materials that would make good wet-mount samples? General 8.What type of eukaryotic cells are the onioncells, elodea cells, and the cells on the prepared slide? 9.Compare and contrast what you saw in animal cells and plant cells. 10.If you did this lab again, what would you do different. V) CONCLUSION What did you learn while doing the Lab? (microscope, cells and organelles you observed, organisms, etc.).Answer in 10-15 complete sentences.