Loading...

Messages

Proposals

Stuck in your homework and missing deadline? Get urgent help in $10/Page with 24 hours deadline

Get Urgent Writing Help In Your Essays, Assignments, Homeworks, Dissertation, Thesis Or Coursework & Achieve A+ Grades.

Privacy Guaranteed - 100% Plagiarism Free Writing - Free Turnitin Report - Professional And Experienced Writers - 24/7 Online Support

Inheritance of sex linked traits worksheet answers

30/03/2021 Client: saad24vbs Deadline: 2 Day

Learn how to perform and interpret the miniaturized, multi-test technique for bacterial identification.

Resource:

Lab manual: http://delrio.dcccd.edu/jreynolds/microbiology/2420/files/API%20%20RAPID%2020E.pdf

BioMerieux, Inc: https://apiweb.biomerieux.com

API 20E Instructions: http://biomanufacturing.org/uploads/files/587872707301898351-api20einstructions.pdf

Pearson MicroLab Tutor Video: API 20E

Introduction:

API-20E test strip (from bioMerieux, Inc.) is a standardized multi-test identification kit for Enterobacteriaceae family members which uses 21 miniaturized biochemical tests and a database (online software or codebook).

API 20 E (API = Analytical Profile Index)

Manufacturer: BioMérieux

Principle:

API test strip consists of 20 microtubes (wells) containing dehydrated testing media.

An unknown bacterial suspension is used to rehydrate and inoculate the media and the strip is incubated.

During incubation, biochemical reactions produce color changes, which are either spontaneous or revealed by the addition of reagents.

The test reactions are read according to the reading table, all + and - test results are compiled to obtain a profile number, which is then compared with the profiles in the codebook or uses online software to determine the identification of the bacterial genus and species.

(oxidase is not part of the strip but counts for the 21st test of API 20E - Enterobacteriaceae members are all negative)

Materials needed:

Setting up API 20E for inoculation and incubation:

Unknown bacterial specimen in agar plate

API 20E test strip

Sterile saline solution to make bacteria suspension

Sterile mineral oil to create anaerobioses for some tests

Reagents for reading API 20E after incubation:

Barrett's reagents A and B for VP test

Kovac's reagent for indole test

10% FeCl3 for TDA test

Pearson MicroLab Tutor Video: API 20E Oversized video in the file “API 20E.mpg” Please play in the file “API 20E.mpg” separately.

https://youtu.be/PXIis18qN9k

https://www.youtube.com/watch?v=ew3amo02_b0

More API videos:

Procedure:

1. Prepare a suspension of unknown bacteria in the saline tube.

Procedure continued:

2. Inoculate the API strip:

All wells - filled with bacteria suspension to the neck.

CIT, VP, and GEL - filled with more bacteria suspension to the top.

ADH, LDC, ODC, H2S, and URE - filled with sterile mineral oil to the top to create anaerobioses for the tests.

Procedure continued:

3. Incubate the strip in its chamber (tray):

Fill the divots of the tray with water to ensure the humidity for the microtubes not to dry out.

Place the strip into the tray.

Cover the chamber with the lid, and label it.

Incubate at 37oC for 18-24 hours.

4. Read test reactions according to the reading table after Incubation:

Add the test reagents to the wells:

1 drop of Barritt's A and B to VP (wait ~ 10 minutes to read)

1 drop of Kovac's to IND

1 drop of FeCl3 to TDA

Read all other tests without reagents.

Record all the results on the report sheet:

20 test reactions (+ or - ) from the strip

21st oxidase negative (-)

Procedure continued:

Case#1: Read the test reactions (+ or -) and record them on the report sh

Homework is Completed By:

Writer Writer Name Amount Client Comments & Rating
Instant Homework Helper

ONLINE

Instant Homework Helper

$36

She helped me in last minute in a very reasonable price. She is a lifesaver, I got A+ grade in my homework, I will surely hire her again for my next assignments, Thumbs Up!

Order & Get This Solution Within 3 Hours in $25/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 3 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

Order & Get This Solution Within 6 Hours in $20/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 6 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

Order & Get This Solution Within 12 Hours in $15/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 12 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

6 writers have sent their proposals to do this homework:

Engineering Exam Guru
Smart Homework Helper
Premium Solutions
Writing Factory
Calculation Master
Essay & Assignment Help
Writer Writer Name Offer Chat
Engineering Exam Guru

ONLINE

Engineering Exam Guru

Give me a chance, i will do this with my best efforts

$65 Chat With Writer
Smart Homework Helper

ONLINE

Smart Homework Helper

I am known as Unrivaled Quality, Written to Standard, providing Plagiarism-free woork, and Always on Time

$125 Chat With Writer
Premium Solutions

ONLINE

Premium Solutions

I have read and understood all your initial requirements, and I am very professional in this task.

$17 Chat With Writer
Writing Factory

ONLINE

Writing Factory

You can award me any time as I am ready to start your project curiously. Waiting for your positive response. Thank you!

$173 Chat With Writer
Calculation Master

ONLINE

Calculation Master

You can award me any time as I am ready to start your project curiously. Waiting for your positive response. Thank you!

$143 Chat With Writer
Essay & Assignment Help

ONLINE

Essay & Assignment Help

I have read your project details. I can do this within your deadline.

$129 Chat With Writer

Let our expert academic writers to help you in achieving a+ grades in your homework, assignment, quiz or exam.

Similar Homework Questions

Oxidation number of al2 so4 3 - Julian marias history of philosophy pdf - Mortimer adler how to mark a book - Need a Rewrite - Caco3 covalent or ionic - Active listening techniques ppt - Care certificate standard 1.2 a answers - Article Critique Paper - What is the difference between financial and managerial accounting - Breaching experiment assignment - Student exploration collision theory worksheet answers - Annotated Bibliography - Nursing. - The pioneers of a warless world - Globalization at the crossroads transcript - Excel work needed - Tupperware replacement parts australia - Use the following cell phone airport data - Business environment examples? - Sample nursing family case study - Features of a newspaper report - IT Ethics and Responsible conduct - Steady state error from graph - 3 page paper on a criminal case - Belmore boy high school - Discussion post in regards to research methods experience from a student point of view. - Data structures and algorithms in java 8 pdf - Southwest airlines human resources strategy - 3 pages needed - Nurs561w4assign - Creating and Organizational Development Proposal - In tray exercise definition - Genie operator training test - An invisible thread cliff notes - Nursing FNP - Marginalized Women and Childbearing Families - Jones design wishes to estimate the value of its - First order system definition - Dyson 1908 2 kb 454 - Great expectations study guide chapters 1 10 answers - Auditing - 182 johnsens road fernbrook - Https www pennfoster edu login - Example of completed r40 form - Open ended questions in nursing - Big mac index china - Talent Management - MATH PROBLEM SET - Cmos schmitt trigger inverter - Organizational design of coca cola - See if i care readworks - Trane leed ce hours - Information systems week 5 - electronic systems in regards to health care - The most contagious diseases and his pharmacological management, - Australian secret intelligence service salary - How to record consignment sales in quickbooks - Igcse biology reproduction past papers - How are accounts in the accounts payable ledger arranged - Mcqs of marketing management by philip kotler 13th edition - Dupont stone and tile floor cleaner - Picot question for short staffing - Family Communication - Will galvanized steel rust in concrete - Lynch company manufactures and sells a single product - DISC 7 415 - Discussion 2.1 - Standardized instruments in social work practice - The breakfast club communication analysis - Threat Modeling - WORLD GEOGRAPHY - How organisational structure influence strategy implementation - Hourly rounding and fall prevention - How to find cuckold couples - What is an analytical sentence - Promotions Management - Target setting display ks2 - Lesson 4.7 flowchart thinking answers - Online discussion - Of all the warlords loved by the gods - Quality points for vegetables - Hyundai iload specs nz - Wedding project charter - Bright star poem summary - El tomate se introdujo a europa como planta ornamental. cierto falso - Four infrastructures of e commerce - Copm assessment occupational therapy - Research PART 1 AND 2 - Scotts miracle gro the spreader sourcing decision case analysis - Enhancer sequences are bound by - The dollhouse murders chapter summaries - Working with political science research methods problems and exercises - Iso 17025 internal audit checklist - Pros and cons of continuing nursing education - Moment of inertia of rectangle - Penn foster writing skills part 3 answers - Advanced project management and strategic leadership - How to find the difference quotient of a function - Academy of government edinburgh - Https www authentichappiness sas upenn edu testcenter - Factor tree of 108