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Microbiology Labs
Lab 2 Introduction to Culturing & Aseptic Technique “BIO250L”
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Access Code (located on the lid of your lab kit): Click here to enter text.
“Experiment 1- Agar Plate Preparation and Bacterial Inoculation”
“Table 1: Experiment 1 Colony Growth”
“Plate #”
“Condition/Surface”
“Growth (color, amount, shape, etc.)”
“1”
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“2”
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“3”
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“4”
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“5”
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“Post-Lab Questions”
“1. Which plate grew the most bacterial species (based on number of different colonies)? Was this a surprise? Why or why not?”
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“2. Was the control plate free of bacterial colonies? If yes, what does this mean for your experiment? If not, what could be the source of the contamination?”
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“3. Did the airborne contamination plate contain any growth? Were any of the colonies similar in appearance to those found on the other plates? How does this plate demonstrate the need for following aseptic technique when culturing bacteria?”
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“Insert photo of your cultures after incubation with your name clearly visible in the background:”
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“Experiment 2- Bacterial Transfer to a Stab Tube and an Agar Plate”
“Table 2: Initial Reserved Plate Colony Growth Observations”
“Colony”
“Plate Sample”
“Appearance (color, morphology, etc.)”
“1”
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“2”
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“3”
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“Table 3: Final Plate and Stab Tube Growth Observations”
“Sample”
“Form”
“Growth?”
“Same Appearance as Initial Plate?”
“Effective Transfer?”
“1”
“Plate”
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“Stab Tube”
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“2”
“Plate”
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“Stab Tube”
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“3”
“Plate”
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“Stab Tube”
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“Control”
“Plate”
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“Stab Tube”
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“Post-Lab Questions”
“1. Did the colonies that grew on the transfer plates retain the same appearance from the initial plate? Why or why not?”
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“2. Were the colonies uniform in appearance on the transfer? What could account for any differences seen?”
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“3. Did growth occur through the depth of the stab tube? If not, what could account for the lack of bacterial growth?”
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“4. Why was it important to include a control plate and stab tube in this experiment? Did they serve as positive or negative controls?”
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“Insert photo of your cultures after incubation with your name clearly visible in the background:”
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