Kiwi dna extraction lab

© Teachable . Some rights reserved. http://teachable.net/res.asp?r=4058Extraction of DNA from kiwi fruit1.Peel your1/4kiwi fruit (don’t need the skin as the cells are mostly dead and do not contain DNA) and cut into pieces2.Mash up your kiwi fruit as muchas possible using a pestle and mortar, or squash in a plastic bag3.Take 25ml of the lysis solution (made by mixing 5g washing up liquid, 2g saltand 100ml tap waterin a beaker) This needs to be stirred slowly to prevent bubbles forming in the solution4.Add the kiwi fruit to your mixture and mash up a bit more 5.Using a glass pencil, write your name on the beaker and put it in a water bath at 60oC for 15 minutes6.Whilst waiting answer the following questions:a)Why do you mash the fruit before extracting DNA?.....................................................................................................................................................................................(1)b)What does the washing up liquid do?.....................................................................................................................................................................................(1)c)What does the salt do?.....................................................................................................................................................................................(1)d)Why do you think bubbles in the solution might be a problem?.....................................................................................................................................................................................(1)e)Why do we heat for 15min in a water bath?.....................................................................................................................................................................................(1)f)What is the purpose of the ethanol?.....................................................................................................................................................................................(1)7.After 15 minutes, remove thebeaker and filter (filter paper and a funnel) into a clean new beaker8.To extract the DNA you can either:Add approx. 5-10ml of this solution to a test-tube and carefully pour an equal volume of ice cold ethanol down the side of the beakerto form a layer on top of the kiwi solutionORadd approx. 5-10ml ice cold ethanol to a test-tube and carefully pour an equal volume of the kiwi solution on top of the ethanol9.Using a paperclipthat has been straightened with a hook on the end, try to hook out the jelly-like substance which is formed and place on a piece of cardg)Describe the DNA you have extracted from the fruit........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................(3)