Laboratory report acid fast staining

LAB REPORT 2- Selected questions from Exercise 3-7 (Acid-fast stain) Note: This is turned in after you have viewed your acid fast stain slides. Bacteria for this exercise are: Mycobacterium smegmatis (or M. phlei) and S. aureus. Q1. Acid fast bacteria are have some significant differences as compared to non-acid fast bacteria. What are the major chemical (structural) differences among these different bacteria that might explain the acid-fast stain? Hint: This structural difference also affects the way the bacteria grow on media (both broth and media containing agar) and the length of time they take to grow. Q2. Why do you suppose the acid fast stain in not as widely used as the Gram stain? When is it more useful than the Gram stain? Can you name the bacterial genera that are considered to be acid fast? Q3. (Use the lab manual to answer this) How does heating the bacterial smear during a Ziehl-Neelson stain promote entry of carbel frshsin into the acid fast cell wall? Why is the Kinyeun procedure referred to as the "cold" acid fast stais procedure? Explain. Q4. Are acid fast negative bacteria stained by carbol fuchsin? If so, explain why the acid fast stain is considered to be a differential stain? LAB REPORT 3-Selected Festions from Exercise 3-9 and hybrid of Ex. 3-5/3-8 (Spore and capsule stain exercises) Note: This lab report is turned in after you have viewed the slides for both the spore stain and the capsule stain (sometimes the capsule stain is dene as a demonstration but you are responsible for looking at a capsule stain slide and knowing how it is done and important to remember, we are using the nigrosin stain procedure as described