Loading...

Messages

Proposals

Stuck in your homework and missing deadline? Get urgent help in $10/Page with 24 hours deadline

Get Urgent Writing Help In Your Essays, Assignments, Homeworks, Dissertation, Thesis Or Coursework & Achieve A+ Grades.

Privacy Guaranteed - 100% Plagiarism Free Writing - Free Turnitin Report - Professional And Experienced Writers - 24/7 Online Support

Microbiology lab questions and answers

02/12/2021 Client: muhammad11 Deadline: 2 Day

Microbiology Lab Report- Gram Staining, Simple Staining, Negative Staining

1. Do a search online 1-2 antibiotics that affect Gram-positive bacteria and list them. On what part of the cell do the antibiotics usually work? List one or two antibiotics that affect Gram-negative bacteria? On what part of the cell do the antibiotics usually work? (Be sure to cite your sources in your answer.) (5 points)

2. Why do you think it is important to identify a bacterial disease in a patient before prescribing any antibiotic treatments? (Be specific.) (5 points)

3. What are some of the limitations of simple staining? (5 points)

4. Give an example of a situation in a lab or medical setting in which simple staining would be utilized. (5 points)

5. So far in this lab, you have used one type of simple stain(Crystal violet) and one type of negative stain (Nigrosin), yet there are many other simple and negative dyes available. Pick one simple dye and one negative dye, and discuss how those dyes differ from the ones you used in this lab. Give a scenario in which their use would be appropriate. (5 points)

6. Using either a textbook or a reputable online resource, research some of the typical characteristics of bacteria, and discuss why it might be important for a researcher or a hospital technician to be able to differentiate between Gram-positive and Gram-negative bacteria. (5 points)

Structure and Microscopy

Lab 4: Structure and Microscopy (100 points)

Student Name:

Student ID:

Course ID:
-Each question on the lab worksheet must be answered completely, thoroughly, in complete sentences and correctly in order to be considered for full credit
-If the question asks you to do research or find a source, a reputable, credible and/or scholarly source citation must be included in order to be considered for full credit
-If a math formula is required to arrive to an answer, work must be shown otherwise, no credit will be awarded
Pre-Lab Questions
1. What determines if a bacterial cell is Gram-positive or Gram-negative? (5 points)

Amount and location of the peptidoglycan molecule in the prokaryotic cell wall determines whether a bacterial cell is Gram-positive or Gram-negative.

2. In this lab, both viruses and prions were introduced as acellular organisms. Do some research and describe one other type of acellular organism. What characteristics about this organism classify it as acellular? (5 points)

Viroids are another type of acellular organism along with viruses and prions. They are plant pathogens, which consist only of a short strand of circular RNA capable of self-replication.

3. Bacteria have many different shapes that often determine their class. Research and form a hypothesis on the evolutionary reasons for so many different bacterial morphologies. (5 points)

Each bacterial morphology may be a selectable feature to aid survival and may have affected by different physical, environmental, and biological forces to contribute to natural selection.

Reference:

Young, K. D. (2006, September). The Selective Value of Bacterial Shape. Retrieved September 30, 2018, from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1594593/

4. Do a search online or look in your textbook for 1-2 antibiotics that affect Gram-positive bacteria and list them. On what part of the cell do the antibiotics usually work? List one or two antibiotics that affect Gram-negative bacteria? On what part of the cell do the antibiotics usually work? (Be sure to cite your sources in your answer.) (5 points)

5. Why do you think it is important to identify a bacterial disease in a patient before prescribing any antibiotic treatments? (Be specific.) (5 points)

d

Experiment 1 Results Tables
Table 1: Experiment 1 Staining Observations (5 points)

Stain used:

Crystal Violet

Observations:

Purple rod-shape bacteria with white background were observed

Experiment 1 Post-Lab Questions
1. How does crystal violet enhance the visualization of microbial features? (5 points)

Crystal violet enhances the contrast between the microorganism itself and the slide, making the bacteria appear as purple.

2. What are some of the limitations of simple staining? (5 points)

3. Give an example of a situation in a lab or medical setting in which simple staining would be utilized. (5 points)

Simple staining is used to obtain basic information about morphology of one type of microorganism through clear visualization.

Experiment 2 Results Tables
Table 2: Experiment 2 Staining Observations (5 points)

Stain used:

Nigrosin

Observations:

Background is stained, bacteria shows up as clear spiral.

Experiment 2 Post-Lab Questions
1. After visualizing the stained samples either using your microscope or by looking at the sample images provided, describe what physical/visual characteristics you were able to observe after performing the negative staining vs. after performing the simple stain. (5 points)

After looking at the sample images provided, negatively stained bacteria showed up as clear straight spirals against a dark background. Bacteria that are simple stained showed up as dark purple rods-shaped with white background.

2. So far in this lab, you have used one type of simple stain and one type of negative stain, yet there are many other simple and negative dyes available. Pick one simple dye and one negative dye, and discuss how those dyes differ from the ones you used in this lab. Give a scenario in which their use would be appropriate. (5 points)

Methylene blue is another dye that can be used for negative stain.

India Ink is another type of negative stain.

Experiment 3 Results Tables
Table 3: Experiment 3 Staining Observations (5 points)

Stain used:

Crystal violet (primary stain) & Safranin (counterstain)

Observations:

Gram-positive appeared as purple and Gram-negative showed up as pink.

Experiment 3 Post-Lab Questions
1. What color are the Gram-positive bacteria after Gram staining? Gram-negative bacteria? (5 points)

Gram-positive bacteria appear as dark purple or blue due to retaining the primary dye (Crystal Violet) in the cell wall.

Gram-negative bacteria appear as red or pink due to decolorizing to accept the counterstain (Safranin).

2. What different characteristic(s) exist between the two groups that account for the different staining conditions? (5 points)

Gram-positive bacteria are stained purple, and gram-negative bacteria stain as pink. They are two distinct morphological groups of bacteria.

3. Why was the Gram iodine added to the Gram staining procedure? (5 points)

Gram iodine is added as a mordant to stabilize the crystal violet iodine complex so that the dye cannot be removed easily.

4. Why is a counterstain (safranin) added to the Gram staining procedure? (5 points)

A counterstain is used to help identify gram-negative bacteria. Gram-negative bacteria lose the crystal violet and stain red.

5. What are the advantages of performing a Gram stain vs. a simple stain for visualizing bacteria? (5 points)

Gram stain contains two or more different stains and can differentiate the species of bacteria into two main groups (gram-positive and gram-negative) by looking at the color of cells (pink or purple). Simple stain involves single stain and it is used to easily determine cell shape, size, and arrangement.

6. Using either a textbook or a reputable online resource, research some of the typical characteristics of bacteria, and discuss why it might be important for a researcher or a hospital technician to be able to differentiate between Gram-positive and Gram-negative bacteria. (5 points)

7. Did you experience any technical difficulties or atypical results during this experiment? If so, what happened, and how could you avoid these issues in the future? (5 points)

Homework is Completed By:

Writer Writer Name Amount Client Comments & Rating
Instant Homework Helper

ONLINE

Instant Homework Helper

$36

She helped me in last minute in a very reasonable price. She is a lifesaver, I got A+ grade in my homework, I will surely hire her again for my next assignments, Thumbs Up!

Order & Get This Solution Within 3 Hours in $25/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 3 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

Order & Get This Solution Within 6 Hours in $20/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 6 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

Order & Get This Solution Within 12 Hours in $15/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 12 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

6 writers have sent their proposals to do this homework:

Homework Master
A Grade Exams
Isabella K.
Professional Coursework Help
Essay & Assignment Help
Engineering Help
Writer Writer Name Offer Chat
Homework Master

ONLINE

Homework Master

I am a PhD writer with 10 years of experience. I will be delivering high-quality, plagiarism-free work to you in the minimum amount of time. Waiting for your message.

$31 Chat With Writer
A Grade Exams

ONLINE

A Grade Exams

I have assisted scholars, business persons, startups, entrepreneurs, marketers, managers etc in their, pitches, presentations, market research, business plans etc.

$24 Chat With Writer
Isabella K.

ONLINE

Isabella K.

As an experienced writer, I have extensive experience in business writing, report writing, business profile writing, writing business reports and business plans for my clients.

$20 Chat With Writer
Professional Coursework Help

ONLINE

Professional Coursework Help

I will be delighted to work on your project. As an experienced writer, I can provide you top quality, well researched, concise and error-free work within your provided deadline at very reasonable prices.

$33 Chat With Writer
Essay & Assignment Help

ONLINE

Essay & Assignment Help

I am an academic and research writer with having an MBA degree in business and finance. I have written many business reports on several topics and am well aware of all academic referencing styles.

$27 Chat With Writer
Engineering Help

ONLINE

Engineering Help

I have assisted scholars, business persons, startups, entrepreneurs, marketers, managers etc in their, pitches, presentations, market research, business plans etc.

$22 Chat With Writer

Let our expert academic writers to help you in achieving a+ grades in your homework, assignment, quiz or exam.

Similar Homework Questions

Utas harvard referencing nursing - Pfa post test answers - Year 1 - bridging coursework - Managerial economics and organizational architecture 6th edition free download - Elastic and inelastic traffic - Produce a change in something - Volume of vehicles theory - Johnny rockabilly has just finished recording - National Patient Safety Goals 250 words with references. Only bids that can complete in 3 hours. - Dallas morning news op ed submission - Z score practice worksheet answers - What can help uncover correlations and trends in data that would otherwise go unrecognized? - Be specific natalie goldberg summary - Caloric content of food labpaq answers - Vroom expectancy theory 1964 - Haply for i am black othello - Rig veda creation hymn summary - A changing world thesis and outline - Book fair report writing - Discussion - Chemistry assignment due by 15 hours - Analysis Essay 2 - Wilsons prom accommodation lighthouse - Compare and contrast democrats and republicans - Competitive advantage of infosys - Project pat walkin bank roll zip - Costco wholesale corporation financial statement analysis b solution - Marketing - Midnight in the garden of good and evil cliff notes - Essential oils for polymyalgia - Phi - 219 grand vista cibolo tx 78108 - Workforce Analysis Paper: Topic Is “Outsourcing” As a culminating experience for this course, each student will be asked to write a 6 page paper, double-spaced, that will be focused on some aspect of employment practices in the 21st century workplace. The - Describe the concept of "Economies of Scale" - Tls ciphertext length must not exceed 2 14 wireshark - What does mrs gren stand for - Theme for english b poem - How would you describe baroque art to someone - Research Process & Methodology - What are the trending human resources philosophies values and policies - Employee management system in c++ using class - Dependability and reliability describe two aspects of the _____ factor of the five-factor model. - What is a satchel of richards meaning - Separation of photosynthetic pigments by paper chromatography lab report - Clints - Rosco cinegel 3409 filter - Green zones moreton bay - Code in color charity kindness diaries - Cpt code for open partial gastrectomy - Procter and gamble global business services case study - Graphics - Steps of fmea process ihi - Gender roles in the crucible - Waverley council clean up - Extra credit - Perennialism philosophy in the classroom - Glen ridge athletic association - Howbo - Chapter 7 - 1984 first chapter summary - WEXL Digital: Elevating Brands with Smart Digital Solutions - By the waters of babylon point of view - Discussion board - Informative speech about dreams outline - Leadership Development - How to become a consultant biomedical scientist - Case study search engines in global business - Structural devices in literature - Food storage in plants biology - A1 page size powerpoint - Passive voice in lab reports - Shankill wellbeing and treatment centre belfast bt13 1pd - V shaped valley diagram - The nutshell studies of unexplained death pdf - Animals should not be kept in cages persuasive text - Compare and contrast unstructured and structured data - The Role of the RN/APRN in Policy Evaluation - Woodcock reading mastery test word attack - I need 3-5 slides about King Saud University. - Www efficientfrontier com files savings path xls - An emission fee levied against polluters will tend to - 2 paragraphs.09/26/20 - Sutton coldfield college design centre - Http www hhmi org biointeractive virus explorer - Example breakdown structure for coffee shop - find two different recent (within 2 years) articles - Ratios and financial planning at east coast yachts solution - A horizontal aluminum rod 4.8 cm in diameter - Number of food banks in canada - How many electrons does germanium have - 11b rutledge crescent quakers hill - Energy australia nmi number - Emergency medicine pico questions - Harry mcclintock ain t we crazy - How can I Hire British ghost writer for my book assignment? - Specialist engineering plastics plc - Optus com au fairgo - What time is 13 35 - 124 ocean view drive wamberal - City of everett washington v mitchell