Loading...

Messages

Proposals

Stuck in your homework and missing deadline? Get urgent help in $10/Page with 24 hours deadline

Get Urgent Writing Help In Your Essays, Assignments, Homeworks, Dissertation, Thesis Or Coursework & Achieve A+ Grades.

Privacy Guaranteed - 100% Plagiarism Free Writing - Free Turnitin Report - Professional And Experienced Writers - 24/7 Online Support

Ph affect enzyme activity lab report

16/10/2021 Client: muhammad11 Deadline: 2 Day

Lab Report

Title: The Effect of Temperature and pH on Enzyme Activity

OBJECTIVE/INTRODUCTION

The purpose of our lab was to observe how the reaction rate of an enzyme increases or decreases when combined with different substrates. Enzymes are catalysts that increase the rate of biochemical reactions. The rate of these reactions can be affected by its environment: temperature and pH. Low temperatures can cause enzyme reaction rate to become inactive or very slow by causing less collision of molecules and high temperatures can cause enzymes to denature prompting the reaction rate to lessen. As temperature increases, the rate of an enzyme-catalyzed reaction should increase. Similarly, the pH of an environment can affect the shape of an enzyme decreasing or increasing its reaction. In addition, all enzymes have a different optimal pH. As pH increases, the rate of an enzyme-catalyzed reaction should increase. In this lab, to examine the effects of temperature and pH on the reaction rate of an enzyme, we utilized potato extract (catecholase) as our enzyme combined with the substrate catechol. We utilized the Spec 20 to determine the reaction rate of enzymes under different conditions because once the enzyme and substrate are combined in the test tube the reaction begins to occur forming benzoquinone. The product benzoquinone is a brown color and the darker the color the less light will be able to pass through the test tube causing the percentage of light absorbed to increase. The absorption of light will be measured by the Spec 20 allowing us to observe the enzymes reaction rate.

MATERIALS/METHOD

Spectrometer

Pipette

Catechol

Potato Extract

Glass Tubes

This lab was a two-part lab where we analyzed the rate of enzyme reactions at different temperatures and later we looked at how pH levels are affected by enzyme-catalyzed reactions. To test the effects of temperature on enzymatic reactions, we filled 5 test tubes with 4 mL of water and 1 mL of potato extract. Then we filled another tube, which we referred to as the blank, with 1 mL of potato extract and 6ml of water (the extra 2 mL of water is added to offset the 2mL of catecholase that will be added later). From here we put all the tubes except the blank, which served as the control of the experiment, into different temperature environments. The temperatures used were 0℃, 18 ℃, 30 ℃, 40 ℃, and 60 ℃. We left the tubes in these conditions for 5 minutes before adding catechol to the tubes then we reinserted the tubes back in these temperature controlled environments for another 5 minutes. The next step after this was to test light absorbance through the use of the Photospectrometer (Spec 20). The higher the absorbance value determined the rate of the chemical reaction. For the section portion of the lab, we tried to see how pH balance affects enzymatic reaction rates. We filled the 5 tubes with 1 mL of potato extract and 4 mL of pH buffer solution. Each tube had varying pH buffer levels. The levels were pH levels of 3, 5, 7, 9, 11 and of course the blank which is our control tube was filled with 1 mL of potato extract and 4 mL of pH 7 buffer which is neutral. We then used parafilm and inverted to mix each solution and added 2 mL of catechol to all 5 test tubes. We waited 5 minutes for the chemical reaction to take place before calibrating the Spec 20 to test the absorbance.

RESULTS

Table 1: Effect of Temperature on Enzyme Reaction Rate

Sample

Temp (℃)

Absorbance after (5 minutes)

1

0℃

.167

2

18℃

.179

3

30℃

.202

4

40℃

.211

5

60℃

.112

Graph 1: Effect of Temperature on Enzyme Reaction Rate

Table 2: Effect of pH on Enzyme Reaction Rate

Sample

pH

Absorbance after (5 minutes)

1

3

.107

2

5

.121

3

7

.197

4

9

.007

5

11

.010

Graph 2: Effect of pH on Enzyme Reaction Rate

As seen in table and graph 1, the effect of temperature on enzyme reaction rate, as the temperature increased the absorbance also increased until it peaked at sample 4 with a temperature of 40℃ which had an absorbance of .211. In the last sample, sample 5 with a temperature of 60℃, the absorbance began to decrease with an absorbance of .112. In table and graph 2, the effect of pH on enzyme reaction rate, the highly acidic samples (1 and 2) had greater absorbance than the basic samples (4 and 5) which had the lowest absorbance out of all the samples. The highest absorbance was in sample 3 (absorbance of .197) which had a neutral pH of 7.

DISCUSSION (need to be done)

Directions: This section should not just be a restatement of the results but should emphasize interpretation of the data, relating them to existing theory and knowledge. Suggestions for the improvement of techniques or experimental design may also be included here. In writing this section, you should explain the logic that allows you to accept or reject your original hypotheses. Provide a conclusion based on the results that you got.

example: My hypothesis was clearly false, as some ions or cofactors had more rates of absorbency than others. This data suggested that copper is the most crucial to the enzymatic reaction as it had the lowest absorbency when bound to prevent reaction. Cofactors such as copper, calcium, and magnesium were tested by using chelating agents to bind them to prevent them from catalyzing them enzymatic reaction. As a result, their efficiency entirely depended on low absorbance rate. Since Tube 4 was the control, we would have thought that it would have the highest absorbance rate as the enzymatic reaction there were no chelating agents binding it.This may have been a possible error such as miscalculation on the portion of the mixture such as having lest EDTA chelating agents, that Tube 1 had fingerprints.There may also be possible errors such as

inaccurate timing mixing the catechol into the solution or the water bath. If this is not the case, we would suspect that there are cofactors of the enzyme that could actually slow down the enzymatic reaction rather than speeds it up. The rate of absorbance was calculated by subtracting the absorbency at 20 minutes to the absorbency at 10 minutes. I concluded this experiment may have possible errors, but suggests that copper bound by Citric acid is the most effective for the

enzymatic reaction of benzoquinone.

Homework is Completed By:

Writer Writer Name Amount Client Comments & Rating
Instant Homework Helper

ONLINE

Instant Homework Helper

$36

She helped me in last minute in a very reasonable price. She is a lifesaver, I got A+ grade in my homework, I will surely hire her again for my next assignments, Thumbs Up!

Order & Get This Solution Within 3 Hours in $25/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 3 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

Order & Get This Solution Within 6 Hours in $20/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 6 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

Order & Get This Solution Within 12 Hours in $15/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 12 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

6 writers have sent their proposals to do this homework:

Accounting Homework Help
Financial Assignments
Professional Coursework Help
University Coursework Help
Write My Coursework
Financial Solutions Provider
Writer Writer Name Offer Chat
Accounting Homework Help

ONLINE

Accounting Homework Help

I am a professional and experienced writer and I have written research reports, proposals, essays, thesis and dissertations on a variety of topics.

$37 Chat With Writer
Financial Assignments

ONLINE

Financial Assignments

I am an experienced researcher here with master education. After reading your posting, I feel, you need an expert research writer to complete your project.Thank You

$35 Chat With Writer
Professional Coursework Help

ONLINE

Professional Coursework Help

I have written research reports, assignments, thesis, research proposals, and dissertations for different level students and on different subjects.

$35 Chat With Writer
University Coursework Help

ONLINE

University Coursework Help

I have read your project details and I can provide you QUALITY WORK within your given timeline and budget.

$16 Chat With Writer
Write My Coursework

ONLINE

Write My Coursework

I am an experienced researcher here with master education. After reading your posting, I feel, you need an expert research writer to complete your project.Thank You

$49 Chat With Writer
Financial Solutions Provider

ONLINE

Financial Solutions Provider

I have worked on wide variety of research papers including; Analytical research paper, Argumentative research paper, Interpretative research, experimental research etc.

$23 Chat With Writer

Let our expert academic writers to help you in achieving a+ grades in your homework, assignment, quiz or exam.

Similar Homework Questions

Education and School Law Order - UDL Modification - Special Topics in Communication Arts Essay 1 - Abbé sieyes what is the third estate - Budleigh salterton medical centre - Organizational Behavior - New Works 10/26 - Oh polly snake charmer - Pulse candy business - The office classical conditioning - Do starfish have organs - Ps music balance sheet - I need 600 words about Save Amazon - Georgia tech housing map - Nick bottom character traits - Tia 942 floor loading capacity - Web server plugin for websphere application server - Tourism marketing mcq with answers - Time domain to phasor calculator - Difference between a leader and a manager in nursing - Hooda math grand prix - Wk 3 - Apply: SSL Lab Using Qualys [due Day 7] - Cars per capita 1 datacamp - Home automation using nodemcu and google assistant - Barcelona restaurant group the evolution of management thinking summary - Probability and statistical inference 9 pdf - Histogram polygon bar graph - British museum's running girl artifact - Euler hermes rating scale 1 10 - Diploma of it rmit - Power triangle for capacitive load - Paper 12 - Strategic management 9th edition dess pdf - Starwind v2v converter linux - Bonaire pyrox spare parts - Data models and decisions the fundamentals of management science download - Peter thieriot - Mgt - Experiment 1: neutralization of acids and bases - Vodafone in egypt case study answers - Big Data analytics - Murdoch university preparation course - Linksys spa2102 power adapter - Medtronic reveal xt 9529 mri safe - Amdahl corporation manufactures large scale high performance computer systems - I/o model of above-average returns - How to build a beam bridge out of popsicle sticks - A child of hitler pdf - Solving trig equations in a given interval - Www baillieuholst com au - What are the seasons on mars - Dalton thomson rutherford and bohr - Accumulated depreciation and depreciation expense are classified respectively as - Business Ethics 6? - Machiavelli book the prince pdf - LITERATURE - 4 2 presentation marketing channel analysis - Qld health hospital map - Indian camp ernest hemingway summary - Australian standards for ceramic tiles - Sears appliance repair 93 w campbell rd rotterdam - BUS Analytics II - Words with home in it - Micro - Business law exam - Uiuc student self service - Trouble in the truss construction shop critical thinking - Idiot nation michael moore analysis - Determination of keq for fescn2+ lab - Macbeth act 3 study questions - Nursing Care Plan - Endorsed components of a training package - One long paragraph each question - According to the lines in bold, what is one reason for schenck’s indictment? - Fin 534 homework set 1 - Kaiser permanente opportunities and threats - Alina, cristina y yo somos buenas amigas. (nosotras) (1) - Health Policy Questions - Why is it desirable to pretest survey instruments - Race car sponsorship template - Positive rational numbers symbol - After reviewing the project scope statement the owner - Http earthquake usgs gov regional nca virtualtour - Proj 587 week 5 case study - Intro psychology online exam - At price p1 the firm in figure 11.1 would produce - Latin name for farmer - Give me liberty an american history 5th edition pdf - Pechenik writing about biology pdf - Week. 3 death - Ethics in information technology by george reynolds 6th edition pdf - Tiger black belt academy hooksett nh - Bics and calp levels - Museprime properties v adhill properties - 2 coments each one 150 words (CITATION AND REFERENCE) - Recall two characters who died of a disease - Inverse of a matrix using gauss jordan method - Administration and Supervision in Criminal Justice - Chapter 10 - How does partnership working deliver better outcomes - Company rms customer number