Spinach tlc chromatography

Hello this lab report is for organic chemistry 1

I have posted the instructions below and I will be uploading some files below for the data please take a deep look at them because this lab report worth a lot of grade so please it needs to be done carefully

If any thing is missed regarding the lab report please let me know

INSTRUCTIONS

Author: (You)

Instructor: (Me)

Section: 691

Date:

(Write your own scientific title)

Introduction

Stick to the main topics: esterification, Fisher esterification, synthesis of acetylsalicylic acid, thin layer chromatography, TLC uses, polarity – don’t get into the medicinal history of acetylsalicylic acid. Keep it simple. There should be citations in this paragraph since this is where background information lives.

There are two parts of this experiment that overlap a little, but aren’t directly connected. If you prefer to use a 3 paragraph structure in order to keep the experimental specifics organized, that is fine. Write a separate hypothesis to end P2 and P3. If you combine both parts of the experiment into a single “In this experiment” paragraph, that’s fine too, just stay organized – clear and concise – you can write two hypotheses at the end of the paragraph, one right after the other.

In the spinach experiment, the main idea is to observe the separation of the spinach pigments and to evaluate the extracted β-carotene to see if it was properly isolated from the other spinach pigments when prepared. In the acetylsalicylic acid synthesis, the main idea is to use TLC, MP, and %yield to determine if the synthesis goes to completion and judge its success.

Terms to include: esterification, mobile phase, stationary phase, salicylic acid, acetylsalicylic acid, retention factor, catalyst (any form of that word), polarity (any form of that word), adsorb (any form of that word).

Materials

Make a list from the lab handout, but also jot down items in your lab notebook while watching the experiment. Everything used should be on this list, including volumes and masses.

Procedures

Numbered list of instructions of how the experiment will be performed. Focus on writing in your own words. Part A and Part B would be appropriate here (or whatever you want to call them).

Data

Photo of experiment notes (name, experiment, date at top of page). Requirements for notes will be discussed during Blackboard meeting. You will need a flow scheme for the ASA synthesis.