Loading...

Messages

Proposals

Stuck in your homework and missing deadline? Get urgent help in $10/Page with 24 hours deadline

Get Urgent Writing Help In Your Essays, Assignments, Homeworks, Dissertation, Thesis Or Coursework & Achieve A+ Grades.

Privacy Guaranteed - 100% Plagiarism Free Writing - Free Turnitin Report - Professional And Experienced Writers - 24/7 Online Support

Triple sugar iron agar results interpretation

03/10/2021 Client: muhammad11 Deadline: 2 Day

Background
Experiment 2: TSI The triple sugar iron agar (TSI) can identify gram negative bacteria based on how they metabolize carbohydrates. It contains three carbohydrates-glucose, lactose and sucrose, in addition to sodium
Background continued...
thiosulfate which some bacteria use in the production of hydrogen sulfide (H2S). It also contains iron in the ferrous form and phenol red as a pH indicator. TSI is poured into a tube when heated to a liquid form, and the tube is slanted as it cools and hardens. When inoculated, a needle transfers microbes down into the tube and also streaked on the slant of the agar surface Glucose is in a smaller quantity than lactose or sucrose and is metabolized first by bacteria. Acids are produced by this metabolism, and the medium changes from red to yellow. As glucose becomes unavailable as it is used up, the surface of the slant will revert back to red if the bacteria cant metabolize lactose or sucrose. This slant would be described as K/A -the slant is red (K or alkaline) and the deep is yellow (A or acidic). If an organism can metabolize all three sugars, the acids produced turn the agar surface yellow and t deep yellow which is described as A/A. Other observations which may be visualized in this agar are the production of gas by bacteria, seen cracks and gaps in the agar, and the production of hydrogen sulfide which appears as a black precipitate in the deep of the tube. All of these metabolic changes are indicative of particular bacte pecies of gram negative bacilli.
Questions
1. Why is the TSI test done? 2. What does TSI stand for? What are the three carbohydrates present in the media? 3. What is the pH indicator in the TSI tube? 4. What do an alkaline slant and an acid deep indicate (K/A)? 5. What do an acid slant and an acid deep indicate (A/A)? 6. What do an alkaline slant and an alkaline deep indicate (K/K)? 7. Why would there be bubbles or breaks in the agar? 8. What does a black color in the media indicate?
Experiment 2: TSI The triple sugar iron agar (TSI) can identify gram negative bacteria based on how they metabolize carbohydrates. It contains three carbohydrates-glucose, lactose and sucrose, in addition to sodium
thiosulfate which some bacteria use in the production of hydrogen sulfide (H2S). It also contains iron in the ferrous form and phenol red as a pH indicator. TSI is poured into a tube when heated to a liquid form, and the tube is slanted as it cools and hardens. When inoculated, a needle transfers microbes down into the tube and also streaked on the slant of the agar surface Glucose is in a smaller quantity than lactose or sucrose and is metabolized first by bacteria. Acids are produced by this metabolism, and the medium changes from red to yellow. As glucose becomes unavailable as it is used up, the surface of the slant will revert back to red if the bacteria can't metabolize lactose or sucrose. This slant would be described as K/A -the slant is red (K or alkaline) and the deep is yellow (A or acidic). If an organism can metabolize all three sugars, the acids produced turn the agar surface yellow and t deep yellow which is described as A/A. Other observations which may be visualized in this agar are the production of gas by bacteria, seen cracks and gaps in the agar, and the production of hydrogen sulfide which appears as a black precipitate in the deep of the tube. All of these metabolic changes are indicative of particular bacte pecies of gram negative bacilli.
1. Why is the TSI test done? 2. What does TSI stand for? What are the three carbohydrates present in the media? 3. What is the pH indicator in the TSI tube? 4. What do an alkaline slant and an acid deep indicate (K/A)? 5. What do an acid slant and an acid deep indicate (A/A)? 6. What do an alkaline slant and an alkaline deep indicate (K/K)? 7. Why would there be bubbles or breaks in the agar? 8. What does a black color in the media indicate?

Homework is Completed By:

Writer Writer Name Amount Client Comments & Rating
Instant Homework Helper

ONLINE

Instant Homework Helper

$36

She helped me in last minute in a very reasonable price. She is a lifesaver, I got A+ grade in my homework, I will surely hire her again for my next assignments, Thumbs Up!

Order & Get This Solution Within 3 Hours in $25/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 3 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

Order & Get This Solution Within 6 Hours in $20/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 6 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

Order & Get This Solution Within 12 Hours in $15/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 12 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

6 writers have sent their proposals to do this homework:

A Grade Exams
Coursework Helper
Premium Solutions
Instant Homework Helper
Assignment Hut
George M.
Writer Writer Name Offer Chat
A Grade Exams

ONLINE

A Grade Exams

Hello, I an ranked top 10 freelancers in academic and contents writing. I can write and updated your personal statement with great quality and free of plagiarism

$46 Chat With Writer
Coursework Helper

ONLINE

Coursework Helper

I am known as Unrivaled Quality, Written to Standard, providing Plagiarism-free woork, and Always on Time

$36 Chat With Writer
Premium Solutions

ONLINE

Premium Solutions

I have read and understood all your initial requirements, and I am very professional in this task.

$23 Chat With Writer
Instant Homework Helper

ONLINE

Instant Homework Helper

Give me a chance, i will do this with my best efforts

$25 Chat With Writer
Assignment Hut

ONLINE

Assignment Hut

I will cover all the points which you have mentioned in your project details.

$40 Chat With Writer
George M.

ONLINE

George M.

Give me a chance, i will do this with my best efforts

$40 Chat With Writer

Let our expert academic writers to help you in achieving a+ grades in your homework, assignment, quiz or exam.

Similar Homework Questions

HISTORICAL APPLICATION OF STATISTIC - 750 10 unc 2a - How to write reflection 1 for english sba - Aca pacific technology m sdn bhd - Satir human validation process model - Can you go to juvie for cyberbullying - Distribution feeder reconfiguration for loss reduction - Electromagnetic spectrum lab report - English Composition 1 - Jennifer aniston aveeno commercial 2019 - MGTU 301 Paper #2 - According to president mandela what is the essence of leadership - Adderall to dexedrine conversion - Trail of the green blazer characters - Average rate of return tutor2u - Penshurst girls high school - Tesco overseas expansion - 7 up or sprite - Soldier Pile and Lagging Wall Analysis – Simplified Method - Mufasa and scar costumes - Which of the following material has maximum ductility answer - The yellow wallpaper secondary sources - Needs assessment - Nitrogen gas and hydrogen gas - Final Disc 4 - What is the earliest surviving european poem - Wireless Systems Assignment - Determine the horizontal component of reaction at pin c - Online community - Humanity - Ingenico ict250 auto settlement - Because you loved me celine dion meaning - Describe one characteristic of a disaster recovery team member - Case study on hr issues with solution - English essay About arguing a policy - Week 5 - Cryokinetics is a rehabilitative technique based on - 3 statement model - Rhetorical analysis essay on a song - College student autobiography sample - Balance sheet exercises with answers - A variable that inadvertently causes an experimental result is - Set notation venn diagrams - Threethorne cattery for sale - Discussion - Flowchart improvement process - Inside planet earth video - Annoated on cybersecurity - The default view in word is ____ - Ops 571 week 5 individual assignment operations forecasting - A pharmaceutical company receives large shipments of aspirin tablets - Phil 347?Argumentive paper - GOAL Setting - Carpal tunnel contents mnemonic - Rsa securid self service portal - Assume the salaries of elementary school teachers - What does the word christian literally mean - New touchstone ( CORRECTION PAPER) - Case study - Operations mangement - Ethical issues in psychological assessment ppt - Width of human hair - Week 5 project - How to do future tense french - Help writing paper - Journal assignment - Total cross sectional area of blood vessels - Hills district farm equipment - A double bubble map - The pearl of love hg wells analysis - Philosophy of man reflection paper - Bnsf contractor orientation test answers - Lifestyle and health practices profile - Tax file declaration question 9 - How to install adobe muse - Http www footprintnetwork org en index php gfn - Sound valid deductive argument in the media - Intel iris pro graphics 5200 photoshop - Sss sas asa rhs - Bcg matrix doc - DIT - Exploring science working scientifically 7 worksheets answers - BlockChain and Crypto Currency - Axess pro 3100 v1 - Business communication building critical skills pdf - Warren hall uc berkeley - Iponz trade mark search - What is a rube goldberg machine definition - Estraven and genly ai - Need help with spanish homework - Need this assignment in COMM120 done by tomorrow - Afr weekend paper price - Patagonia five forces analysis - Research article critique apa format - 2011 hsc economics answers - 929 727 5499 - Effective practices for managers and supervisors - Jelly bean trailer park wasaga beach - The book thief images - Facial recognition software its reliability effectiveness and admissibility