130 Unit in Blood Collection Procedures MATCHING Use choices only once unless otherwise indicated. MATCHING 7-1: KEY TERMS AND DESCRIPTIONS Match each key term with the best description. Key Terms ACD Additive Anticoagulants Antiglycolytic agent Antiseptics Bevel Biocide Blood collection sets Carry-over/cross-contamination Clear/discard tube Descriptions A. Abbreviation for the colle used for routine venipun B. Additive that prevents the by the cells C. Additive used for collecting D. Additive used for immunoh DNA and HLA typing E. Anticoagulant that inhibits thrombin F. Anticoagulant that preserves the structure and inhibits platelet G. Breakdown or metabolism of cells H. Chemical that kills or inhibits the organisms 1. Coagulation-enhancing substance, se silica J. Number that is related to the diamez the needle lumen K. Point of a needle that is cut on a slant skin entry L. Solutions used to kill microorganisms and instruments M. Special winged and nonwinged need attached tubing N. Substance added to a blood collect O. Substances that prevent blood from P. Substances used for skin cleaning tu growth of bacteria Q. Term for a tube drawn to removed tubing and then thrown away R. Transfer of additive from one tube S. Tubes with premeasured vacuum stoppers Clot activator CPD Disinfectants EDTA ETS Evacuated tubes Gauge Glycolysis Heparin 19. -
132 Unit Blood Collection Procedures ITS PRIMARY FUNCTION MATCHING 7-2: MATCH THE ADDITIVE WITH ITS PRIMARY Choices may be used more than once. Primary Function A. Anticoagulant B. Antiglycolytic agent C. Clot activator D. Serum/plasma separator Additive ACD CPD EDTA Heparin Potassium oxalate Silica Sodium citrate Sodium fluoride SPS 10.- Thixotropic gel Additive MATCHING 7-3: MATCH THE ADDITIVE WITH THE TYPE OF ACTION Choices may be used more than once. Type of Action A. Binds calcium B. Enhances coagulation Heparin C. Inhibits thrombin D. Physical barrier E. Preserves glucose Citrate EDTA Potassium oxalate Silica Sodium fluoride Thixotropic gel Tube:
137 Chapter 7 Blood Collection Equipment, Additives, and Order of Draw KNOWLEDGE DRILL 7-3: TRUE/FALSE ACTIVITY The following statements are all false. Circle the one or two words that make the statement false, and write the correct word(s) that would make the statement true in the space provided. 1. Use of Band-Aids to hold pressure is not recommended because they tend to stick to the site and reinitiate bleeding when removed because they dislodge the platelet plug that seals a puncture site. 2. A properly applied tourniquet is tight enough to restrict arterial flow out of the area but not so tight as to restrict venous flow into the area. 3. A needle or tube holder that has a safety device is an example of a SESIP, which is the CLIA acronym for a sharp with engineered sharps injury protection. 4. When drawing venous blood by syringe, the phlebotomist slowly pulls back the plunger, creating a vacuum that causes the shaft to fill with blood. 5. A transfer device must be held horizontal when tubes are being filled to prevent blood in the tube from touching the needle within the transfer device. 6. An additive tube must be gently inverted two to four times, depending on the type of additive and the manufacturer, immediately after collection to adequately mix the additive with the specimen. 7. Vigorous mixing or an excessive number of inversions in light blue tubes can activate white cells and lengthen clotting times. 8. When using the ETS system, royal blue tops for trace element studies should be collected first to avoid even the smallest amount of carryover. 9. The most common antimicrobial agent is sodium fluoride. It preserves glucose and inhibits the growth of bacteria. 10. Sodium fluoride in tubes has been the source of more carryover problems than any other additive. II. Glycolysis occurs faster in newborns because their metabolism is decreased. 12. Coagulation tubes require a 9:1 ratio of additive to blood. 13. Coagulation specimens are placed on a mechanical rotator to keep them from settling out. 14. Using a needle with a diameter larger than a 25-gauge increases the chance of hemolysis. 15. The FDA has banned the use of latex gloves in medicine because they pose a danger to human health.
OWLEDGE DRILL 7-4: STOPPER COLORS, ADDITIVES, AND DEPARTMENTS Fill in the blanks of the following table with the missing information Stopper Colors) Department(s) Additive Light blue Sodium citrate Chemistry, blood bank.nl Red (glass) immunology (1) SKILI SKILLS Identity stopper Testi Clot activator NA (discard purpose only) Red (plastic) Red/light gray Clear Red/black (tiger) Chemistry Clot activator and (6) Lithium heparin and gel separator Green/gray Chemistry Green (8) Lithium heparin Sodium heparin EDTA (9) Lavender (purple) (10) Gray EDTA Sodium fluoride and Blood bank Chemistry Sodium fluoride and (12) Sodium fluoride (13) Orange Chemistry Gray/yellow Royal blue (14) Chemistry (15) Sodium (16) (17) Tan Yellow Chemistry Microbiology (18) (SPS) Acid citrate dextrose Yellow (20) (19) Immunohematology