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UMUC Biology 102/103
Lab 3: Cell Structure and Function
INSTRUCTIONS:
• On your own and without assistance, complete this Lab 3 Answer Sheet electronically and submit it via the Assignments Folder by the date listed in the Course Schedule (under Syllabus).
• To conduct your laboratory exercises, use the Laboratory Manual located under Course Content. Read the introduction and the directions for each exercise/experiment carefully before completing the exercises/experiments and answering the questions.
• Save your Lab 3 Answer Sheet in the following format: LastName_Lab3 (e.g., Smith_Lab3).
• You should submit your document as a Word (.doc or .docx) or Rich Text Format (.rtf) file for best compatibility.
Pre-Lab Questions
1. Identify three major similarities and differences between prokaryotic and eukaryotic cells.
2. Where is the DNA housed in a prokaryotic cell? Where is it housed in a eukaryotic cell?
3. Identify three structures which provide support and protection in a eukaryotic cell.
Experiment 1: Cell Structure and Function
The structure of a cell dictates the majority of its function. You will view a selection of slides that exhibit unique structures that contribute to tissues function.
Materials:
Onion (allium) Root Digital Slide Images
Procedure
1. Examine the onion root tip digital slide images on the following pages. Then, respond to the Post-Lab Questions.
Onion Root Tip: 100X
Onion Root Tip: 1000X
Onion Root Tip: 1000X
Onion Root Tip: 100X. Each dark circle indicates a different nucleus.
Onion Root Tip: 1000X
Post-Lab Questions
1. Label each of the arrows in the following slide image: A=Chromosomes, B=Nucleus, C=Cytoplasm, D=Cell Wall
2. What is the difference between the rough and smooth endoplasmic reticulum?
3. Would an animal cell be able to survive without a mitochondria? Why or why not?
4. What could you determine about a specimen if you observed a slide image showing the specimen with a cell wall, but no nucleus or mitochondria?
5. Hypothesize why parts of a plant, such as the leaves, are green, but other parts, such as the roots, are not. Use scientific reasoning to support your hypothesis.
Experiment 2: Osmosis - Direction and Concentration Gradients
In this experiment, we will investigate the effect of solute concentration on osmosis. A semi-permeable membrane (dialysis tubing) and sucrose will create an osmotic environment similar to that of a cell. This selective permeability allows us to examine the net movement of water across the membrane. You will begin the experiment with a 30% sucrose solution, and perform a set of serial dilutions to create lower concentration solutions. Some of the sucrose concentrations will be membrane permeable; while others will not be permeable (can you determine why this is?).