Loading...

Messages

Proposals

Stuck in your homework and missing deadline? Get urgent help in $10/Page with 24 hours deadline

Get Urgent Writing Help In Your Essays, Assignments, Homeworks, Dissertation, Thesis Or Coursework & Achieve A+ Grades.

Privacy Guaranteed - 100% Plagiarism Free Writing - Free Turnitin Report - Professional And Experienced Writers - 24/7 Online Support

Why don t microorganisms in cultures exhibit constant exponential growth

29/10/2021 Client: muhammad11 Deadline: 2 Day

“Lab 2 Culturing & Aseptic Technique BIO250L”

Student Name: Click here to enter text.

Access Code (located on the underside of the lid of your lab kit): Click here to enter text.

“Pre-Lab Questions”

1. Proper aseptic technique is crucial to ensuring growth of pure bacterial colonies. What is one experimental way you can test your practices to confirm that you are using proper aseptic technique? Click here to enter text.

2. In a laboratory setting, what are three ways you can properly sterilize culturing equipment?

a. Click here to enter text.

b. Click here to enter text.

c. Click here to enter text.

3. For each inoculation tool, give one scenario in which use of that tool would be appropriate. Click here to enter text.

4. Why don’t microorganisms in cultures exhibit constant exponential growth? What are some steps you could take to extend the lifespan of a microbial culture? Click here to enter text.

5. Using a textbook or a reputable online source, describe how lab cultures are maintained in a continual pattern of growth. Focus particularly on those used in biotechnology, such as E. coli, which is used to make human insulin. Click here to enter text.

6. Which of these has a constant growth pattern: an open system or a closed system? Click here to enter text.

7. A human patient represents what kind of system for bacterial infections? Click here to enter text.

8. You’re a physician trying to isolate bacterial colonies from the human gut in attempt to diagnose a gastrointestinal infection. You streak your sample on a growth media containing glucose, amino acids, and salts that contain both sulfur and phosphorous with a pH of 7. You incubate the plates in aerobic conditions at 37 ˚C for three days, at which point you can see clear bacterial colonies forming on the plate. Would you feel confident in stating that you had successfully cultured all the bacteria from your gut sample? Why or why not? Click here to enter text.

“EXPERIMENT 1: Agar Plate Preparation and Bacterial Inoculation

Data Tables
Table 1: Experiment 1 Colony Growth

Plate Number

Source

Growth

(Color, Amount, Shape, etc.)

1

Click here to enter text.

Click here to enter text.

2

Click here to enter text.

Click here to enter text.

3

Click here to enter text.

Click here to enter text.

4

Click here to enter text.

Click here to enter text.

5

Click here to enter text.

Click here to enter text.

Post-Lab Questions
1. Do some research and try to identify one to three types of microbes cultured on your plates. Using any resources available to you (i.e., a textbook or internet sources such as the CDC website), look up what bacteria are usually found on the areas from which you obtained your samples and the morphological characteristics of their colonies. Describe what morphological traits (i.e., size, shape, arrangement, color, margin, etc.) led to your hypothesized species identification. Be as specific as possible in the microbe type. Click here to enter text.

2. For the colonies hypothesized in Question 1, specify which plate and source the microbes came from. Are you surprised to find this type of microbe on this surface? Click here to enter text.

3. Looking at your control, did you perform proper aseptic technique or were your plates contaminated? If contamination did occur, list the possible sources and how you can prevent contamination in the future. Click here to enter text.

4. Was there a large risk for airborne contamination of your experimental plates? Based on the colonies that grew on your plates, do you think any of your experiment plates received airborne contamination? Click here to enter text.

Insert a photo of your plates after incubation. Include your name and access code handwritten in the background of your photo.

“EXPERIMENT 2: Bacterial Transfer to a Stab Tube and an Agar Plate

Data Tables
Table 2: Initial Reserved Plate Colony Growth Observations

Plate Sample

Appearance (morphology, etc.)

1

Click here to enter text.

2

Click here to enter text.

3

Click here to enter text.

Table 3: Final Plate and Stab Tube Growth Observations

Sample

Form

Growth (Yes or No)

Same Appearance as Initial Plate (Yes or No)

Successful Transfer? (Yes or No)

1

Plate

1

Stab Tube

2

Plate

2

Stab Tube

3

Plate

3

Stab Tube

Control

Plate

Control

Stab Tube

Post-Lab Questions
1. Were all of your colony transfers successful? Explain what could have been the cause of any unsuccessful transfers. Click here to enter text.

2. Did you have any growth that was different in appearance from the initial plates? What might account for any differences in growth on the transfer plate/tube? Click here to enter text.

3. Describe why an unsuccessful transfer or growth of the transfer plate/tube differing from the initial plates would be a problem if this experiment were placed in a larger context (i.e., only one step in a longer experiment). Click here to enter text.

4. Do some research and describe two or three scenarios in which it would be preferable to use a stab tube vs. a growth plate. (Hint: What do bacteria use to help them move? Can motility be used to help identify many medically important pathogenic bacteria such as the Enterobacteriaceae?) Click here to enter text.

Insert a photo of your plates and stab tubes after incubation. Include your name and access code handwritten in the background of your photo.

Homework is Completed By:

Writer Writer Name Amount Client Comments & Rating
Instant Homework Helper

ONLINE

Instant Homework Helper

$36

She helped me in last minute in a very reasonable price. She is a lifesaver, I got A+ grade in my homework, I will surely hire her again for my next assignments, Thumbs Up!

Order & Get This Solution Within 3 Hours in $25/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 3 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

Order & Get This Solution Within 6 Hours in $20/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 6 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

Order & Get This Solution Within 12 Hours in $15/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 12 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

6 writers have sent their proposals to do this homework:

Write My Coursework
Finance Master
Instant Assignment Writer
Calculation Master
Top Quality Assignments
Unique Academic Solutions
Writer Writer Name Offer Chat
Write My Coursework

ONLINE

Write My Coursework

After reading your project details, I feel myself as the best option for you to fulfill this project with 100 percent perfection.

$35 Chat With Writer
Finance Master

ONLINE

Finance Master

I will be delighted to work on your project. As an experienced writer, I can provide you top quality, well researched, concise and error-free work within your provided deadline at very reasonable prices.

$42 Chat With Writer
Instant Assignment Writer

ONLINE

Instant Assignment Writer

I find your project quite stimulating and related to my profession. I can surely contribute you with your project.

$50 Chat With Writer
Calculation Master

ONLINE

Calculation Master

I will provide you with the well organized and well research papers from different primary and secondary sources will write the content that will support your points.

$46 Chat With Writer
Top Quality Assignments

ONLINE

Top Quality Assignments

I am an experienced researcher here with master education. After reading your posting, I feel, you need an expert research writer to complete your project.Thank You

$37 Chat With Writer
Unique Academic Solutions

ONLINE

Unique Academic Solutions

I reckon that I can perfectly carry this project for you! I am a research writer and have been writing academic papers, business reports, plans, literature review, reports and others for the past 1 decade.

$24 Chat With Writer

Let our expert academic writers to help you in achieving a+ grades in your homework, assignment, quiz or exam.

Similar Homework Questions

Risk assessment for office based activities - Beverly name meaning hebrew - Bossard international inc - Psychology test - Mkt 571 week 1 individual assignment - X 5 2x 15 0 - Australian qualifications framework at certificate iv - Hachi kanji stroke order - A mis padres les gusta ir a yucatán porque - Ascending tracts in the spinal cord - National textile university faisalabad fee structure - Communication based on a speaker's body and voice - Technology mandatory units of work - How to win erp simulation game - Shadow health focused exam cough - Bs 5950 part 1 2000 - 555 california tree lighting - Advanced financial accounting and reporting - Papa model risk management - How to provide culturally sensitive nursing care - How do solar cooker relate to conics - Disaster Recovery Best Practices - What is ftk imager used for - Product Strategy Discussion - Charmate tex offset smoker review - Physics and astronomy st andrews - Finance Exam / Need it within 10 hours / Complete in a Word document - Managing operations across the supply chain 3rd edition test bank - Gomez runs a small pottery firm - Tests for oxygen hydrogen and carbon dioxide - 5 w's of fever - Iso 4063 weld symbol - Personal leadership - Conduct a risk assessment based on the information from this presentation - Ms mikami homework - Hilti hvu installation instructions - Walmart unethical behavior cases - Andrew noah trevor noah's brother - D addario string tension chart - I need a discussion about this topic - Due tomorrow------Accounting - Integration by substitution worksheet doc - Silver bromide exposed to sunlight - Goal setting theory of motivation - 7 habits of highly effective teams - Plan or review administration systems assessment - Huey j theus lithographs the past remembered - Human behavior negative and positive effects on the environment - Need official letter for absent - Discussion response - Business - Est quickstart qs1 data sheet - Basic networking tutorial for beginners pdf - Who can complete this assignment by 11:59pm tomorrow August 4th? - A key text in support of trichotomism is… - Against school john taylor gatto thesis - Flipkart supply chain case study - Moody gardens 20000 leagues under the sea - Does under armour have any core competencies - Nelson chemistry 12 solutions - Solution focused therapy session transcript - 36 phyllis frost street forde - Queensland christian soccer association - Yarn measure equivalent to 18 hanks - Cloud computing - Annotated Bibliography for the Article Review Final Paper - Causes of misbehavior or disruptive behavior in the inclusion classroom - Which of the following typifies decision making in bands - Adaptations of palisade cells - Java magazine 2017 pdf - Sealink palm island ferry timetable - Business organization - Smallest positive solution maple - Apple and its suppliers case study - Is everyone really equal second edition - HIS 121 - Paper - Pursuit Of Happiness - If a violin string vibrates at hz - Defining crime: graphic or visual - Interrogative adjectives in english - How to write a plaafp - Library Assignment - The frost of death was on the pane analysis - Describe the concept of "Economies of Scale" - Literacy Toolkit 1: Phonics and Word Recognition - Discussion - Music grant proposal sample - Double bubble map example - Caloric content of food labpaq answers - Windshield Survey Response 2 - Barnes and noble annual report - Frosted flakes marketing strategy - Deb the travel doctor - Turner's budget furniture moultrie ga - Ernestine wiedenbach nursing theory pdf - What is the null factor law - Hr case study scenarios worksheet answers - Write Analysis of a KM consulting report 3 pages - 510 discussion 1 - Larkrise boarding cattery trowbridge