Loading...

Messages

Proposals

Stuck in your homework and missing deadline? Get urgent help in $10/Page with 24 hours deadline

Get Urgent Writing Help In Your Essays, Assignments, Homeworks, Dissertation, Thesis Or Coursework & Achieve A+ Grades.

Privacy Guaranteed - 100% Plagiarism Free Writing - Free Turnitin Report - Professional And Experienced Writers - 24/7 Online Support

Why don t microorganisms in cultures exhibit constant exponential growth

14/10/2021 Client: muhammad11 Deadline: 2 Day

“Lab 2 Culturing & Aseptic Technique BIO250L”

Student Name: Click here to enter text.

Access Code (located on the underside of the lid of your lab kit): Click here to enter text.

“Pre-Lab Questions”

1. Proper aseptic technique is crucial to ensuring growth of pure bacterial colonies. What is one experimental way you can test your practices to confirm that you are using proper aseptic technique? Click here to enter text.

2. In a laboratory setting, what are three ways you can properly sterilize culturing equipment?

a. Click here to enter text.

b. Click here to enter text.

c. Click here to enter text.

3. For each inoculation tool, give one scenario in which use of that tool would be appropriate. Click here to enter text.

4. Why don’t microorganisms in cultures exhibit constant exponential growth? What are some steps you could take to extend the lifespan of a microbial culture? Click here to enter text.

5. Using a textbook or a reputable online source, describe how lab cultures are maintained in a continual pattern of growth. Focus particularly on those used in biotechnology, such as E. coli, which is used to make human insulin. Click here to enter text.

6. Which of these has a constant growth pattern: an open system or a closed system? Click here to enter text.

7. A human patient represents what kind of system for bacterial infections? Click here to enter text.

8. You’re a physician trying to isolate bacterial colonies from the human gut in attempt to diagnose a gastrointestinal infection. You streak your sample on a growth media containing glucose, amino acids, and salts that contain both sulfur and phosphorous with a pH of 7. You incubate the plates in aerobic conditions at 37 ˚C for three days, at which point you can see clear bacterial colonies forming on the plate. Would you feel confident in stating that you had successfully cultured all the bacteria from your gut sample? Why or why not? Click here to enter text.

“EXPERIMENT 1: Agar Plate Preparation and Bacterial Inoculation

Data Tables
Table 1: Experiment 1 Colony Growth

Plate Number

Source

Growth

(Color, Amount, Shape, etc.)

1

Click here to enter text.

Click here to enter text.

2

Click here to enter text.

Click here to enter text.

3

Click here to enter text.

Click here to enter text.

4

Click here to enter text.

Click here to enter text.

5

Click here to enter text.

Click here to enter text.

Post-Lab Questions
1. Do some research and try to identify one to three types of microbes cultured on your plates. Using any resources available to you (i.e., a textbook or internet sources such as the CDC website), look up what bacteria are usually found on the areas from which you obtained your samples and the morphological characteristics of their colonies. Describe what morphological traits (i.e., size, shape, arrangement, color, margin, etc.) led to your hypothesized species identification. Be as specific as possible in the microbe type. Click here to enter text.

2. For the colonies hypothesized in Question 1, specify which plate and source the microbes came from. Are you surprised to find this type of microbe on this surface? Click here to enter text.

3. Looking at your control, did you perform proper aseptic technique or were your plates contaminated? If contamination did occur, list the possible sources and how you can prevent contamination in the future. Click here to enter text.

4. Was there a large risk for airborne contamination of your experimental plates? Based on the colonies that grew on your plates, do you think any of your experiment plates received airborne contamination? Click here to enter text.

Insert a photo of your plates after incubation. Include your name and access code handwritten in the background of your photo.

“EXPERIMENT 2: Bacterial Transfer to a Stab Tube and an Agar Plate

Data Tables
Table 2: Initial Reserved Plate Colony Growth Observations

Plate Sample

Appearance (morphology, etc.)

1

Click here to enter text.

2

Click here to enter text.

3

Click here to enter text.

Table 3: Final Plate and Stab Tube Growth Observations

Sample

Form

Growth (Yes or No)

Same Appearance as Initial Plate (Yes or No)

Successful Transfer? (Yes or No)

1

Plate

1

Stab Tube

2

Plate

2

Stab Tube

3

Plate

3

Stab Tube

Control

Plate

Control

Stab Tube

Post-Lab Questions
1. Were all of your colony transfers successful? Explain what could have been the cause of any unsuccessful transfers. Click here to enter text.

2. Did you have any growth that was different in appearance from the initial plates? What might account for any differences in growth on the transfer plate/tube? Click here to enter text.

3. Describe why an unsuccessful transfer or growth of the transfer plate/tube differing from the initial plates would be a problem if this experiment were placed in a larger context (i.e., only one step in a longer experiment). Click here to enter text.

4. Do some research and describe two or three scenarios in which it would be preferable to use a stab tube vs. a growth plate. (Hint: What do bacteria use to help them move? Can motility be used to help identify many medically important pathogenic bacteria such as the Enterobacteriaceae?) Click here to enter text.

Insert a photo of your plates and stab tubes after incubation. Include your name and access code handwritten in the background of your photo.

Homework is Completed By:

Writer Writer Name Amount Client Comments & Rating
Instant Homework Helper

ONLINE

Instant Homework Helper

$36

She helped me in last minute in a very reasonable price. She is a lifesaver, I got A+ grade in my homework, I will surely hire her again for my next assignments, Thumbs Up!

Order & Get This Solution Within 3 Hours in $25/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 3 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

Order & Get This Solution Within 6 Hours in $20/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 6 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

Order & Get This Solution Within 12 Hours in $15/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 12 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

6 writers have sent their proposals to do this homework:

Helping Engineer
Unique Academic Solutions
Math Guru
Accounting & Finance Master
Academic Master
Quick Finance Master
Writer Writer Name Offer Chat
Helping Engineer

ONLINE

Helping Engineer

I find your project quite stimulating and related to my profession. I can surely contribute you with your project.

$41 Chat With Writer
Unique Academic Solutions

ONLINE

Unique Academic Solutions

As per my knowledge I can assist you in writing a perfect Planning, Marketing Research, Business Pitches, Business Proposals, Business Feasibility Reports and Content within your given deadline and budget.

$33 Chat With Writer
Math Guru

ONLINE

Math Guru

I have written research reports, assignments, thesis, research proposals, and dissertations for different level students and on different subjects.

$42 Chat With Writer
Accounting & Finance Master

ONLINE

Accounting & Finance Master

After reading your project details, I feel myself as the best option for you to fulfill this project with 100 percent perfection.

$32 Chat With Writer
Academic Master

ONLINE

Academic Master

I am a professional and experienced writer and I have written research reports, proposals, essays, thesis and dissertations on a variety of topics.

$33 Chat With Writer
Quick Finance Master

ONLINE

Quick Finance Master

I am a PhD writer with 10 years of experience. I will be delivering high-quality, plagiarism-free work to you in the minimum amount of time. Waiting for your message.

$15 Chat With Writer

Let our expert academic writers to help you in achieving a+ grades in your homework, assignment, quiz or exam.

Similar Homework Questions

Class ab output stage - St rebecca hospital asaba - Collective bargaining analysis - Testout power supply lab - When driving 55 mph how long to stop - Problem solving - Essay - Explain the meanings of total architecture and machines for living - Which metal is the strongest reducing agent - Cloud Computing - Spring multiple choice questions and answers - 53 robinsville crescent thirroul - 2.05 bar to psi - Peak envelope power vs average power - 3 day lesson plan - Bin day moreton bay - Compute the ending inventory at september - Snap core first tutorial - Writers such as shakespeare and marlowe focused on - Facts about grand canyon - Is graffiti art or vandalism argument essay - Dcc concepts point wiring - Physical flow of inventory - Graham woodhouse conveyancing burnie tas - Cdt cutting oil sds - How do you write 12 noon am or pm - What is xix in numbers - Wogs out of work nick giannopoulos - Mit 12 subjects csu - Sas proc format cntlout - Vnxe3200 latest firmware version - Ocr nationals ict level 2 - Fiscal Planing and Budgeting - Naval letter format template - Abs and abdc journal rankings - Code of the street elijah anderson chapter 2 summary - Gai tronics auteldac 5 - Campaign assignment - Crime Analysis Data Worksheet - Beer pong rules australia - Plant chromatography lab answers - Principles of curriculum design - Thandi coaches times from birmingham to southall - Gymnastics apparatus lesson plans - 31 gunjet spray gun - Security policy related to a bomb threat - Practical Connection Assignment - 500 words - Peanut company acquired 90 percent of snoopy company's outstanding - Ts3200 tape library express - Lección 3 la familia answers - Evidence based practice project - Marriott corporation case study solution - Models for writers short essays for composition 13th edition pdf - The final step in the screening process will generally include - How to use emulsifier in cake - Human remains pompeii and herculaneum - Printed legacy media - Residency Research Paper - Paint estimator java - What can limit the stylistic choices of a filmmaker - 15th century painting of a market in the netherlands - Managerial Economics - Introduction to java 11th edition pdf - Describe the importance of effective communication in the correctional setting - A little water clears us of this deed literary device - Due 10/4/2020 1000 words advance directive in healthcare - Spanish alphabet pronunciation sheet - Socw 6443 Project: Final Paper proposal: Questions and Controversies in Clinical Psychopharmacology - Communicating effectively with aboriginal and torres strait islander people - Lopilato bros hume - Is the executive summary on a separate page - Literature review - Fin 571 week 3 quiz - What three elements did faber feel were missing from life - Hisense fridge freezer troubleshooting - Unchained our family's addiction mess is our message - Kookie kutter bakery case study - Criteria for weighted scoring model - Global supply chain management simulation solution - Unit 8 p3 e commerce - Where do sheldon and leonard live in pasadena - The purchase of supplies on account should result in - Liberty life cover brochure - Essay - California residential purchase agreement and joint escrow instructions 2015 - Special consideration for exams - Alphabetic telephone number translator python - Reflection - Mother any distance annotations - Answer any one of the questions below: - Geoforum journal impact factor - Silicon labs cp210x driver - Summary and personal reflection (due on Sp.15th) - Barium chloride magnesium nitrate - Juniper ssg 550 eol - Math 20 2 inductive and deductive reasoning - Sociology discussion - Netflix Project Metrics - Gerry gibbs camera house cannington wa - Ethical concepts and ethical issues in the automotive industry to support your point of view