Loading...

Messages

Proposals

Stuck in your homework and missing deadline?

Get Urgent Writing Help In Your Essays, Assignments, Homeworks, Dissertation, Thesis Or Coursework & Achieve A+ Grades.

Privacy Guaranteed - 100% Plagiarism Free Writing - Free Turnitin Report - Professional And Experienced Writers - 24/7 Online Support

Gram stain lab report results

05/01/2021 Client: saad24vbs Deadline: 2 Day

Y:\ESL Kits\Manual Graphics\PlainHdr.jpg


Structure and Microscopy


Lab 4: Structure and Microscopy (100 points)


Student Name:


Student ID:


Course ID:

-Each question on the lab worksheet must be answered completely, thoroughly, in complete sentences and correctly in order to be considered for full credit

-If the question asks you to do research or find a source, a reputable, credible and/or scholarly source citation must be included in order to be considered for full credit

-If a math formula is required to arrive to an answer, work must be shown otherwise, no credit will be awarded

Pre-Lab Questions

1. What determines if a bacterial cell is Gram-positive or Gram-negative? (5 points)


Amount and location of the peptidoglycan molecule in the prokaryotic cell wall determines whether a bacterial cell is Gram-positive or Gram-negative.


2. In this lab, both viruses and prions were introduced as acellular organisms. Do some research and describe one other type of acellular organism. What characteristics about this organism classify it as acellular? (5 points)


Viroids are another type of acellular organism along with viruses and prions. They are plant pathogens, which consist only of a short strand of circular RNA capable of self-replication.


3. Bacteria have many different shapes that often determine their class. Research and form a hypothesis on the evolutionary reasons for so many different bacterial morphologies. (5 points)


Each bacterial morphology may be a selectable feature to aid survival and may have affected by different physical, environmental, and biological forces to contribute to natural selection.


Reference:


Young, K. D. (2006, September). The Selective Value of Bacterial Shape. Retrieved September 30, 2018, from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1594593/


4. Do a search online or look in your textbook for 1-2 antibiotics that affect Gram-positive bacteria and list them. On what part of the cell do the antibiotics usually work? List one or two antibiotics that affect Gram-negative bacteria? On what part of the cell do the antibiotics usually work? (Be sure to cite your sources in your answer.) (5 points)


5. Why do you think it is important to identify a bacterial disease in a patient before prescribing any antibiotic treatments? (Be specific.) (5 points)


d


Experiment 1 Results Tables

Table 1: Experiment 1 Staining Observations (5 points)


Stain used:


Crystal Violet


Observations:


Purple rod-shape bacteria with white background were observed


Experiment 1 Post-Lab Questions

1. How does crystal violet enhance the visualization of microbial features? (5 points)


Crystal violet enhances the contrast between the microorganism itself and the slide, making the bacteria appear as purple.


2. What are some of the limitations of simple staining? (5 points)


3. Give an example of a situation in a lab or medical setting in which simple staining would be utilized. (5 points)


Simple staining is used to obtain basic information about morphology of one type of microorganism through clear visualization.


Experiment 2 Results Tables

Table 2: Experiment 2 Staining Observations (5 points)


Stain used:


Nigrosin


Observations:


Background is stained, bacteria shows up as clear spiral.


Experiment 2 Post-Lab Questions

1. After visualizing the stained samples either using your microscope or by looking at the sample images provided, describe what physical/visual characteristics you were able to observe after performing the negative staining vs. after performing the simple stain. (5 points)


After looking at the sample images provided, negatively stained bacteria showed up as clear straight spirals against a dark background. Bacteria that are simple stained showed up as dark purple rods-shaped with white background.


2. So far in this lab, you have used one type of simple stain and one type of negative stain, yet there are many other simple and negative dyes available. Pick one simple dye and one negative dye, and discuss how those dyes differ from the ones you used in this lab. Give a scenario in which their use would be appropriate. (5 points)


Methylene blue is another dye that can be used for negative stain.


India Ink is another type of negative stain.


Experiment 3 Results Tables

Table 3: Experiment 3 Staining Observations (5 points)


Stain used:


Crystal violet (primary stain) & Safranin (counterstain)


Observations:


Gram-positive appeared as purple and Gram-negative showed up as pink.


Experiment 3 Post-Lab Questions

1. What color are the Gram-positive bacteria after Gram staining? Gram-negative bacteria? (5 points)


Gram-positive bacteria appear as dark purple or blue due to retaining the primary dye (Crystal Violet) in the cell wall.


Gram-negative bacteria appear as red or pink due to decolorizing to accept the counterstain (Safranin).


2. What different characteristic(s) exist between the two groups that account for the different staining conditions? (5 points)


Gram-positive bacteria are stained purple, and gram-negative bacteria stain as pink. They are two distinct morphological groups of bacteria.


3. Why was the Gram iodine added to the Gram staining procedure? (5 points)


Gram iodine is added as a mordant to stabilize the crystal violet iodine complex so that the dye cannot be removed easily.


4. Why is a counterstain (safranin) added to the Gram staining procedure? (5 points)


A counterstain is used to help identify gram-negative bacteria. Gram-negative bacteria lose the crystal violet and stain red.


5. What are the advantages of performing a Gram stain vs. a simple stain for visualizing bacteria? (5 points)


Gram stain contains two or more different stains and can differentiate the species of bacteria into two main groups (gram-positive and gram-negative) by looking at the color of cells (pink or purple). Simple stain involves single stain and it is used to easily determine cell shape, size, and arrangement.


6. Using either a textbook or a reputable online resource, research some of the typical characteristics of bacteria, and discuss why it might be important for a researcher or a hospital technician to be able to differentiate between Gram-positive and Gram-negative bacteria. (5 points)


7. Did you experience any technical difficulties or atypical results during this experiment? If so, what happened, and how could you avoid these issues in the future? (5 points)

Homework is Completed By:

Writer Writer Name Amount Client Comments & Rating
Instant Homework Helper

ONLINE

Instant Homework Helper

$36

She helped me in last minute in a very reasonable price. She is a lifesaver, I got A+ grade in my homework, I will surely hire her again for my next assignments, Thumbs Up!

Guarantee Your Academic Success!

Hire Professional Writers And Get Best Homework Writing Services In Any Subject

6 writers have sent their proposals to do this homework:

Helping Hand
Top Essay Tutor
University Coursework Help
Writer Writer Name Offer Chat
Helping Hand

ONLINE

Helping Hand

I am an Academic writer with 10 years of experience. As an Academic writer, my aim is to generate unique content without Plagiarism as per the client’s requirements.

$110 Chat With Writer
Top Essay Tutor

ONLINE

Top Essay Tutor

I have more than 12 years of experience in managing online classes, exams, and quizzes on different websites like; Connect, McGraw-Hill, and Blackboard. I always provide a guarantee to my clients for their grades.

$115 Chat With Writer
University Coursework Help

ONLINE

University Coursework Help

Hi dear, I am ready to do your homework in a reasonable price.

$112 Chat With Writer

Let our expert academic writers to help you in achieving a+ grades in your homework, assignment, quiz or exam.

Similar Homework Questions

Which means Erath - Brooke building salford royal - Discussion Board - How to turn recurring decimals into fractions - Curtin university grades distinction - Action Plan - Compared to the purely competitive firm a pure monopoly - Paper - Frankenstein Critical Analysis Paper - Gary ball can t pay take it away - MGT-322: Logistics Management assignment - Somebody wanted but so then finally - 28 beliefs of sda - Ccna 200-301 exam questions pdf - Public Health Policy Analysis (PHPA) Paper - How might we statements - Childbirth positive feedback loop diagram - Exhibition budgerigar world magazine - Produces top selling chessmen crossword clue - MG401 Leadership Unit 5 Discussion - A los puertorriqueños les encanta visitar los ríos del yunque. - Covalent bonds of a carbon atom crossword - Research Paper 4 - St james episcopal church warrenton va - Fizzy song bugsy malone - A statistical method for identifying cost behavior is the: - Terrorism and bitcoin - Modern taxonomists today tend to group organisms into the same category based on - Are penn foster exams timed - Neil ashe net worth - What is installed when you choose the rsat-dfs-mgmt-con feature? - H&m's supply chain management practices - Video questions for an inconvenient truth answers - Miss marple the pale horse full episode - Beef cattle quiz questions - Romeo and juliet writing prompts - Bul3130 - Projectile motion pre lab investigation answers - PTSD Powerpoint - This is what it means to say phoenix arizona theme - The treadmill of consumption james roberts summary - What is the cause of fatigue for the alactacid system - Rusty spotted cat hybrid - Po box 4 gloucester gl4 3rl - Quantitative - Solve 2x 13 8x 7 - Arabic to english translation in riyadh - Supply chain - Formal and informal groups ppt - Supply chain management - Critical Analysis Essay - Research Paper: COSO Framework - Topic 5: Emergency Preparedness and Disaster Management - I need 800 words answering the questions - A small backpack manufacturer carries four different - IT - Benchmark- Nursing Process: Approach to Care - D4 - Page and a half response for tomorrow - 31.24 to 1 decimal place - Ecu apa referencing guide pdf - 20 hand signals in basketball with description - How to create a fixture - Supply chain management information system - Los estudiantes de la universidad virtual estudian - Example of 6x6 rule powerpoint - Management internal control toolset login - Determination of molecular weight by boiling point elevation - Grand canyon university nursing reviews - Boardworks science chemistry ionic bonding worksheet answers - Example of scholarly activity in nursing - Miss clairol short story - Human rights consortium scotland - Butchers block greeneville tennessee menu - Econ 312 week 1 quiz - Jasper jones jeffrey lu cricket - Cidb malaysia contractor grading table - Emotional uplifting orchestral osu - Application support process improvement - Chi square practice problems - Agilent hplc software openlab - Critical Thinking Chapter 11 (4-5 Pages) - Criminological Theory - Non elastic impression materials - What is guided writing in primary school - Employee training and development noe 5th edition pdf - The engaged citizen ou custom ed 1 by andrea lunsford - Finance Discussion Question - As 1170 structural design actions - Learning intentions and success criteria examples - Fisher exact test calculator - Divorce lawyer in lahore - Nursing. - Archer daniels midland and the friendly competitors case study - Assignment: Surveys and Response Rates - A voyage to the country of the houyhnhnms pdf - Mornington peninsula planning zones - Woodlands junior tudor food - Kid punches wall modern warfare 2 - Seek australia cv template