Loading...

Messages

Proposals

Stuck in your homework and missing deadline? Get urgent help in $10/Page with 24 hours deadline

Get Urgent Writing Help In Your Essays, Assignments, Homeworks, Dissertation, Thesis Or Coursework & Achieve A+ Grades.

Privacy Guaranteed - 100% Plagiarism Free Writing - Free Turnitin Report - Professional And Experienced Writers - 24/7 Online Support

Microbiology lab report gram stain

21/10/2021 Client: muhammad11 Deadline: 2 Day

Microbiology Lab Report- Gram Staining, Simple Staining, Negative Staining

1. Do a search online 1-2 antibiotics that affect Gram-positive bacteria and list them. On what part of the cell do the antibiotics usually work? List one or two antibiotics that affect Gram-negative bacteria? On what part of the cell do the antibiotics usually work? (Be sure to cite your sources in your answer.) (5 points)

2. Why do you think it is important to identify a bacterial disease in a patient before prescribing any antibiotic treatments? (Be specific.) (5 points)

3. What are some of the limitations of simple staining? (5 points)

4. Give an example of a situation in a lab or medical setting in which simple staining would be utilized. (5 points)

5. So far in this lab, you have used one type of simple stain(Crystal violet) and one type of negative stain (Nigrosin), yet there are many other simple and negative dyes available. Pick one simple dye and one negative dye, and discuss how those dyes differ from the ones you used in this lab. Give a scenario in which their use would be appropriate. (5 points)

6. Using either a textbook or a reputable online resource, research some of the typical characteristics of bacteria, and discuss why it might be important for a researcher or a hospital technician to be able to differentiate between Gram-positive and Gram-negative bacteria. (5 points)

Structure and Microscopy

Lab 4: Structure and Microscopy (100 points)

Student Name:

Student ID:

Course ID:
-Each question on the lab worksheet must be answered completely, thoroughly, in complete sentences and correctly in order to be considered for full credit
-If the question asks you to do research or find a source, a reputable, credible and/or scholarly source citation must be included in order to be considered for full credit
-If a math formula is required to arrive to an answer, work must be shown otherwise, no credit will be awarded
Pre-Lab Questions
1. What determines if a bacterial cell is Gram-positive or Gram-negative? (5 points)

Amount and location of the peptidoglycan molecule in the prokaryotic cell wall determines whether a bacterial cell is Gram-positive or Gram-negative.

2. In this lab, both viruses and prions were introduced as acellular organisms. Do some research and describe one other type of acellular organism. What characteristics about this organism classify it as acellular? (5 points)

Viroids are another type of acellular organism along with viruses and prions. They are plant pathogens, which consist only of a short strand of circular RNA capable of self-replication.

3. Bacteria have many different shapes that often determine their class. Research and form a hypothesis on the evolutionary reasons for so many different bacterial morphologies. (5 points)

Each bacterial morphology may be a selectable feature to aid survival and may have affected by different physical, environmental, and biological forces to contribute to natural selection.

Reference:

Young, K. D. (2006, September). The Selective Value of Bacterial Shape. Retrieved September 30, 2018, from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1594593/

4. Do a search online or look in your textbook for 1-2 antibiotics that affect Gram-positive bacteria and list them. On what part of the cell do the antibiotics usually work? List one or two antibiotics that affect Gram-negative bacteria? On what part of the cell do the antibiotics usually work? (Be sure to cite your sources in your answer.) (5 points)

5. Why do you think it is important to identify a bacterial disease in a patient before prescribing any antibiotic treatments? (Be specific.) (5 points)

d

Experiment 1 Results Tables
Table 1: Experiment 1 Staining Observations (5 points)

Stain used:

Crystal Violet

Observations:

Purple rod-shape bacteria with white background were observed

Experiment 1 Post-Lab Questions
1. How does crystal violet enhance the visualization of microbial features? (5 points)

Crystal violet enhances the contrast between the microorganism itself and the slide, making the bacteria appear as purple.

2. What are some of the limitations of simple staining? (5 points)

3. Give an example of a situation in a lab or medical setting in which simple staining would be utilized. (5 points)

Simple staining is used to obtain basic information about morphology of one type of microorganism through clear visualization.

Experiment 2 Results Tables
Table 2: Experiment 2 Staining Observations (5 points)

Stain used:

Nigrosin

Observations:

Background is stained, bacteria shows up as clear spiral.

Experiment 2 Post-Lab Questions
1. After visualizing the stained samples either using your microscope or by looking at the sample images provided, describe what physical/visual characteristics you were able to observe after performing the negative staining vs. after performing the simple stain. (5 points)

After looking at the sample images provided, negatively stained bacteria showed up as clear straight spirals against a dark background. Bacteria that are simple stained showed up as dark purple rods-shaped with white background.

2. So far in this lab, you have used one type of simple stain and one type of negative stain, yet there are many other simple and negative dyes available. Pick one simple dye and one negative dye, and discuss how those dyes differ from the ones you used in this lab. Give a scenario in which their use would be appropriate. (5 points)

Methylene blue is another dye that can be used for negative stain.

India Ink is another type of negative stain.

Experiment 3 Results Tables
Table 3: Experiment 3 Staining Observations (5 points)

Stain used:

Crystal violet (primary stain) & Safranin (counterstain)

Observations:

Gram-positive appeared as purple and Gram-negative showed up as pink.

Experiment 3 Post-Lab Questions
1. What color are the Gram-positive bacteria after Gram staining? Gram-negative bacteria? (5 points)

Gram-positive bacteria appear as dark purple or blue due to retaining the primary dye (Crystal Violet) in the cell wall.

Gram-negative bacteria appear as red or pink due to decolorizing to accept the counterstain (Safranin).

2. What different characteristic(s) exist between the two groups that account for the different staining conditions? (5 points)

Gram-positive bacteria are stained purple, and gram-negative bacteria stain as pink. They are two distinct morphological groups of bacteria.

3. Why was the Gram iodine added to the Gram staining procedure? (5 points)

Gram iodine is added as a mordant to stabilize the crystal violet iodine complex so that the dye cannot be removed easily.

4. Why is a counterstain (safranin) added to the Gram staining procedure? (5 points)

A counterstain is used to help identify gram-negative bacteria. Gram-negative bacteria lose the crystal violet and stain red.

5. What are the advantages of performing a Gram stain vs. a simple stain for visualizing bacteria? (5 points)

Gram stain contains two or more different stains and can differentiate the species of bacteria into two main groups (gram-positive and gram-negative) by looking at the color of cells (pink or purple). Simple stain involves single stain and it is used to easily determine cell shape, size, and arrangement.

6. Using either a textbook or a reputable online resource, research some of the typical characteristics of bacteria, and discuss why it might be important for a researcher or a hospital technician to be able to differentiate between Gram-positive and Gram-negative bacteria. (5 points)

7. Did you experience any technical difficulties or atypical results during this experiment? If so, what happened, and how could you avoid these issues in the future? (5 points)

Homework is Completed By:

Writer Writer Name Amount Client Comments & Rating
Instant Homework Helper

ONLINE

Instant Homework Helper

$36

She helped me in last minute in a very reasonable price. She is a lifesaver, I got A+ grade in my homework, I will surely hire her again for my next assignments, Thumbs Up!

Order & Get This Solution Within 3 Hours in $25/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 3 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

Order & Get This Solution Within 6 Hours in $20/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 6 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

Order & Get This Solution Within 12 Hours in $15/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 12 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

6 writers have sent their proposals to do this homework:

Smart Tutor
Fatimah Syeda
Ideas & Innovations
Top Rated Expert
A+GRADE HELPER
Innovative Writer
Writer Writer Name Offer Chat
Smart Tutor

ONLINE

Smart Tutor

I have worked on wide variety of research papers including; Analytical research paper, Argumentative research paper, Interpretative research, experimental research etc.

$32 Chat With Writer
Fatimah Syeda

ONLINE

Fatimah Syeda

I will be delighted to work on your project. As an experienced writer, I can provide you top quality, well researched, concise and error-free work within your provided deadline at very reasonable prices.

$23 Chat With Writer
Ideas & Innovations

ONLINE

Ideas & Innovations

I have written research reports, assignments, thesis, research proposals, and dissertations for different level students and on different subjects.

$42 Chat With Writer
Top Rated Expert

ONLINE

Top Rated Expert

Being a Ph.D. in the Business field, I have been doing academic writing for the past 7 years and have a good command over writing research papers, essay, dissertations and all kinds of academic writing and proofreading.

$15 Chat With Writer
A+GRADE HELPER

ONLINE

A+GRADE HELPER

I will provide you with the well organized and well research papers from different primary and secondary sources will write the content that will support your points.

$17 Chat With Writer
Innovative Writer

ONLINE

Innovative Writer

As per my knowledge I can assist you in writing a perfect Planning, Marketing Research, Business Pitches, Business Proposals, Business Feasibility Reports and Content within your given deadline and budget.

$21 Chat With Writer

Let our expert academic writers to help you in achieving a+ grades in your homework, assignment, quiz or exam.

Similar Homework Questions

Note - Paper on Big Data and Internet of Things - What did henry hess invent - Soap for sinners chelsea market - What are the conventions of shakespearean comedy - Mri pre screening questionnaire - Voyage of despair shield parts - Topic 6 insolation and the seasons answers - Ucsd 2016 2017 calendar - What is trepanning in psychology - Astro malaysia holdings berhad annual report 2011 - Altech uec remote control model b3 - How to find solubility from ksp - 1 page single space paper - How does a fuming chamber work - Pt tata consultancy services indonesia - Schizophrenia - Research Methods in Accounting and Finance - Create your own ecological model - Metro renal case study - DQ1 DQ2. BY9/22 - Simon from lord of the flies - GHF A4 - Jcpenney rebate forms cooks 2016 - Nova power surge video worksheet answers - Psy - Ifsm 300 stage 2 cic hiring process - Romeo and juliet study guide questions and answers act 2 - Write and read - Business - Operations management simulation inventory basics answers - Book Review - Optus com au fairgo - Lab Report - Red panda food chain - Business assignment - Case study - Pontiac two speeches 1762 and 1763 - Resource manager and variation - Why did dr karl kruszelnicki migrate to australia - One – Group Assessment - Single axle roll off hoist - An iron boiler of mass 180 kg - Parker posey breakfast club - Can a breeder repossess a dog - Trends & issues in executive management for health care administrators - Equip with knowledge synonym - Factor pairs of 2 - - Community Teaching Plan: Community Teaching Work Plan Proposal - Ashfield medical centre doctors - Circulant matrix matlab - A company began its fiscal year with inventory of - Imo sample papers for class 7 - Bhatiary golf and country club - Coursework-Technical Report on Professional Computing - Collins vocabulary for ielts - Justice peter hely scholarship - Eso error 102 invalid metafile - Fly chicago skydiving - ASSIGNMENT 5 - Psychclassics yorku ca maslow motivation htm - To kill a mockingbird trial questions - Cloud Computing - The autobiography of yukichi fukuzawa pdf - Complete children's health moonee ponds - How to increase SDLC - Murray library sunderland address - Individual awareness paper, including an abstract and a bibliography - Give an acceptable iupac name for the following compound - Hid proxpoint plus 6005 wiring diagram - Dulux st clair half - DiscussionC 3-1 - Bandicoot holes in garden - Do not uniquely identify observations in the master data - Honeypot medical centre doctors - Cada tres horas sale un barco de la habana con destino a regla. cierto falso - Reply - Nike wages in china - Dandenong ranges national park walks - Float type rain gauge - Primary Document Analysis - Order # 9363 - Fully automatic can crusher - Business Policy and Strategy III Case Study - Elleebana one shot lash lift instructions - Systematic theology paper topics - Los baker van a peru sparknotes - The murder city devils in san francisco slim's december 31 - Declaration of contribution thesis - 62a queenstown avenue boondall - HTML & css , etc. website creation - 500 word Research Report - Macroeconomic 1 - Apply the soft edges 5 pt picture effect - Delta electronics singapore pte ltd - How to make a pill bug habitat - What are proteins made up of monomers - All about wales powerpoint - Jem glaciator dynamic manual - Clinical coding refresher course