Loading...

Messages

Proposals

Stuck in your homework and missing deadline? Get urgent help in $10/Page with 24 hours deadline

Get Urgent Writing Help In Your Essays, Assignments, Homeworks, Dissertation, Thesis Or Coursework & Achieve A+ Grades.

Privacy Guaranteed - 100% Plagiarism Free Writing - Free Turnitin Report - Professional And Experienced Writers - 24/7 Online Support

What happens to mrna after it completes transcription

08/11/2021 Client: muhammad11 Deadline: 2 Day

Gene Expression Lab Simulation worksheet adapted by L. McPheron & Shannon Nixon; Phet Simulation by Elizabeth Hobbs; Mutation worksheet by Eliza Woo

Objectives:

● Identify the roles transcription factors, RNA polymerase, ribosomes, and mRNA destroyers have on transcription and translation.

● Distinguish between the location and function of regulatory regions compared to transcribed regions of DNA.

● Predict the effects of concentration, affinity, and degradation rates of transcription factors and RNA polymerase on gene expression.

● Identify the effects of mutations on gene expression. Background: Transcription​ is the process of making mRNA from DNA. This is a highly regulated process that our cells complete in preparation to make a protein. ​Translation​ is the process of making a protein from a piece of mRNA.

DNA --------------------> mRNA --------------------> protein transcription translation

Not all regions of DNA are used to make mRNA - only the parts of DNA that correspond to genes. Even then, not all gene regions are transcribed all the time. When genes are transcribed into mRNA depends on the needs of the cell. Once mRNA is made from DNA, it is translated into protein. Translation is an energy expensive process (it requires LOTS of ATP) which is one reason the cell only completes the process when the protein product is needed. This week’s “Reading and Lesson” explains many of the details of these highly complicated processes, transcription and translation. Please review the lesson for a deeper understanding of the concepts in this lab activity. Procedure: Click the Play arrow on this ​Gene Expression activity​ to complete the simulations. (The simulations are also embedded in the Canvas lab assignment page.) You will complete 3 simulations: 1) Expression, 2) mRNA, and 3) Multiple Cells.

Part 1: Expression Simulation

Click “Expression” to start that simulation. Notice the molecule that spans across the screen, from left to right. Answer the following 2 questions:

1. What is this molecule that spans across the page that is shown in red and blue?

2. What do you think the different colors (red and blue) of the molecule represent?

1

https://phet.colorado.edu/en/simulation/gene-expression-essentials
Now, start the process of transcription.

For transcription, you need these things to happen. First, most genes require 1 or 2 “transcription factors” to bind to the area in front of the gene (called the “regulatory region”). Second, an RNA polymerase (an enzyme that makes mRNA from DNA) needs to be present in order for transcription to occur.

1. Drag one Positive Transcription Factor and one RNA Polymerase from the box called Biomolecule Toolbox to the regulatory region on the DNA molecule. This should start TRANSCRIPTION.

2. Now, drag a ribosome next to the mRNA, in order to do TRANSLATION. 3. The mRNA is eventually broken down by an mRNA destroyer protein. Drag one of these next to the

mRNA when it is done making a protein. 4. Put the protein in Your Protein Collection. 5. Stop the gene from working by dragging the Negative Transcription Factor to the Regulatory Area, and

remove the Positive Transcription Factor by dragging it out of the way.

After you have made 1 protein, answer these 5 questions. HINT: Think about what/where things are at the start, and what/ where things are at the end of the process.

1. What does the “Positive Transcription Factor” do?

2. What does the “RNA Polymerase” do?

3. What does the “Ribosome” do?

4. What does the “mRNA destroyer” do?

5. What does the “negative transcription” factor do?

2

Click the yellow “Next Gene” box to begin working on the second gene. Can you remember the steps in order from your first trial? Try to see if you can! (HINT: There is one small difference between the transcription of gene 2 versus gene 1 - the difference is not in the order of steps but in the amount of something!) If not, not to worry, we are still learning… As a reminder, the steps are:

1. Drag Positive Transcription Factors and one RNA Polymerase from the box called Biomolecule Toolbox to the regulatory region on the DNA molecule. This should start TRANSCRIPTION!

2. Now, drag a ribosome next to the mRNA, in order to do TRANSLATION! 3. The mRNA is eventually broken down by an mRNA destroyer protein. Drag one of these next to the

mRNA when it is done making a protein. 4. Put the protein in Your Protein Collection. 5. Stop the gene from working by dragging the Negative Transcription Factor to the Regulatory Area, and

remove the Positive Transcription Factors by dragging them out of the way.

After you have made the second protein, answer these 2 questions.

1. What is one difference you noticed that was required to initiate the transcription of gene 2 versus gene 1?

2. What could be an advantage of multiple positive transcription factors versus only one?

Now, put all of your items back in the Biomolecule Toolbox and begin again, and answer the following 2 questions.

1. What happens if you add 2 RNA Polymerases (one after the first, before transcription is complete), and then 2 ribosomes (one for each mRNA)?

2. What would be the benefit of working this way versus adding RNA Polymerase one at a time?

Click the yellow “Next Gene” box to begin working on the third gene. Can you remember the steps in order from your first trial? Try to see if you can!

3

Additional 4 Questions from the Expression Simulation:

1. What is gene expression?

2. What molecules are involved in gene expression? List them all and state the role of each.

3. What is the difference between the “regulatory region” and the “transcribed region”?

4. A student says that “ALL DNA codes for proteins.” Do you agree with her? Why or why not? Give evidence to support your answer.

Part 2: mRNA Simulation

At the bottom of the simulation page, click on the next simulation (it’s greyed out) called mRNA.

You should see a strand of DNA with a bunch of RNA Polymerases floating around. (If the RNA Polymerases are not moving, click the Play button.) Answer the following 7 questions.

1. Is mRNA being made?

2. In the Positive Transcription Factor box, slide the Concentration slider from NONE to just a tad (a couple millimeters or so) away from NONE. What do you notice is happening in the simulation now?

4

3. Move the Concentration slider all the way to HIGH. How does this affect what is happening in the simulation?

4. Leave the Concentration slider on HIGH but move the Affinity slider all the way to LOW. What happens? Move the Affinity slider to a midway setting? What happens now? Based on these observations, what do you think ​affinity​ means in this simulation?

5. Place both sliders in the Positive Transcription Factor box on the HIGH setting. ​Predict ​what will happen to the simulation if you were to move the RNA Polymerase affinity slider to the LOW position. Record your prediction.

6. Now, move the RNA Polymerase affinity slider to the LOW position and record your observations. Was your prediction correct?

7. Place all the sliders in the HIGH position. Check the box to add Negative Transcription Factors and place the concentration and affinity sliders on HIGH. How does this change transcription compared to without Negative Transcription Factors?

Continue to play around with the sliders until you can accurately predict how the change will affect transcription each time.

5

Additional 3 Questions from the mRNA Simulation:

1. What circumstances make the most mRNA? (What slider positions?)

2. What circumstances make the least mRNA? (What slider positions?)

3. Why would a cell need the option to make or not make a protein?

Part 3: Multiple Cells Simulation

At the bottom of the simulation page, click on the next simulation (it’s greyed out) called Multiple Cells.

Watch the generation of the graph called Average Protein Level vs. Time when one cell is working. If the graph does not automatically begin, then click the Play button at the bottom of the page. Answer the following 4 questions.

1. On the right side of the page, there are controls for Concentration, Affinity, and Degradation. (You need to click the green + to see the sliders.) Predict where you need to place each of the 3 sliders to achieve lots of protein. Record your predictions here:

a. The Concentration slider should be on LOW or on HIGH to achieve lots of protein?

b. The Affinity slider should be on LOW or on HIGH to achieve lots of protein?

c. The Degradation slider should be on LOW or on HIGH to achieve lots of protein?

6

2. Now, move the sliders into the positions you predicted to see if your predictions were correct. (NOTE: Each time you click “Refresh” to restart the graph, all of the sliders reset themselves to their original setting.) Then, explain why each setting - concentration, affinity, and degradation - makes sense for making lots of protein.

3. Why would a protein need to be degraded?

4. Think back to last week’s lab – Lactase Enzyme Lab. Give an example from that lab of a time when it would be necessary to make a lot of one type of protein.

Part 4: Effects of Mutations on Gene Expression You have learned this week that cells use the two-step process of transcription and translation to transform a protein-coding DNA sequence into a chain of amino acids that makes up a protein. The resulting chain of amino acids will fold into a three-dimensional protein structure that defines the phenotype. Imagine that the following DNA sequence is part of a protein-coding gene. Use this sequence to answer the questions that follow.

… G G A T G C C G C T C T G C A A C T A C...

A) What is the ​complementary DNA sequence​ to the DNA sequence above? ​Hint: look back to your reading and lesson notes to recall the pairing rules for nucleotides A, T, G, and C if you need to!

B) What is the ​mRNA sequence​ transcribed from the DNA sequence from ​Part A​? ​Hint: your answer below should start with the letter ​G​ and not ​C​!

C) What ​corresponding amino acid sequence​ is translated from the mRNA sequence from ​Part (B)​? Use the genetic code from the lesson or the one posted in the lab. ​Remember that your amino acid sequence should always start with the ​START codon​!

D) For the following scenarios (i)-(iii), identify the type of mutation that has occurred (single base-pair substitution or frameshift mutation) to our original sequence AND the new amino acid chain that results

7

from such a mutation. Complete the same sequence from complementary DNA sequence, then mRNA sequence, and then corresponding amino acid sequence like what you did in Parts A, B, and C above!

(i) The 4​th​ C in the original sequence is mutated to a T:

… G G A T G C C G C T ​T​ T G C A A C T A C ...

Type of mutation:

New amino acid chain:

(ii) An extra C is inserted into the original sequence:

… G G A T G C C G C ​C​ T C T G C A A C T A C ...

Type of mutation:

New amino acid chain:

(iii) The 5​th​ C in the original sequence is mutated to A:

… G G A T G C C G C T C T G ​A​ A A C T A C ...

Type of mutation:

New amino acid chain:

E) At the end of translation, an amino acid chain will subsequently fold into a protein with a specific structure and function.

(i) Of the three mutations described in part (D), which mutation will cause the ​least ​change to protein function? Briefly explain your reasoning.

(ii) Which mutation would you expect to significantly alter protein function? Briefly explain your reasoning.

Homework is Completed By:

Writer Writer Name Amount Client Comments & Rating
Instant Homework Helper

ONLINE

Instant Homework Helper

$36

She helped me in last minute in a very reasonable price. She is a lifesaver, I got A+ grade in my homework, I will surely hire her again for my next assignments, Thumbs Up!

Order & Get This Solution Within 3 Hours in $25/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 3 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

Order & Get This Solution Within 6 Hours in $20/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 6 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

Order & Get This Solution Within 12 Hours in $15/Page

Custom Original Solution And Get A+ Grades

  • 100% Plagiarism Free
  • Proper APA/MLA/Harvard Referencing
  • Delivery in 12 Hours After Placing Order
  • Free Turnitin Report
  • Unlimited Revisions
  • Privacy Guaranteed

6 writers have sent their proposals to do this homework:

Engineering Exam Guru
Top Grade Essay
Online Assignment Help
Accounting Homework Help
24/7 Assignment Help
Solutions Store
Writer Writer Name Offer Chat
Engineering Exam Guru

ONLINE

Engineering Exam Guru

I will provide you with the well organized and well research papers from different primary and secondary sources will write the content that will support your points.

$43 Chat With Writer
Top Grade Essay

ONLINE

Top Grade Essay

I have read your project details and I can provide you QUALITY WORK within your given timeline and budget.

$30 Chat With Writer
Online Assignment Help

ONLINE

Online Assignment Help

I can assist you in plagiarism free writing as I have already done several related projects of writing. I have a master qualification with 5 years’ experience in; Essay Writing, Case Study Writing, Report Writing.

$30 Chat With Writer
Accounting Homework Help

ONLINE

Accounting Homework Help

I am an academic and research writer with having an MBA degree in business and finance. I have written many business reports on several topics and am well aware of all academic referencing styles.

$15 Chat With Writer
24/7 Assignment Help

ONLINE

24/7 Assignment Help

I have read your project details and I can provide you QUALITY WORK within your given timeline and budget.

$35 Chat With Writer
Solutions Store

ONLINE

Solutions Store

As an experienced writer, I have extensive experience in business writing, report writing, business profile writing, writing business reports and business plans for my clients.

$24 Chat With Writer

Let our expert academic writers to help you in achieving a+ grades in your homework, assignment, quiz or exam.

Similar Homework Questions

Last draft - Scholastic literacy pro library student login - Discussion: Ethics in Military Practice socw 6212 wk1 - Receiver coffee victoria row - Biology - What is a producer in an ecosystem - What is inappropriate communication - List four characteristics of a suspended process - Paper - Milestone 3 - Optimize in a sentence - Caravan and camping show exhibitors - Scalded dog runs away after being boiled alive - Econ quizes - Final 6 - Psychological treatment plan template - Psychology exam - Discussion - "A" WORK DISCUSSION IN 15 HOURS - How did fizeau calculate the speed of light - Evidence - Hazpak risk assessment tool - What is the root cause of human problems cwv - Risk management - Public Administration - Broken bulk prescription example - Nutrition and Weight Status - Copssh 3.1 4 download - English composition - Is sdram better than dram - JA2 - Regal river falls stadium 12 nobody's fool - Drift and papillion apartments byron bay - Iturralde v hilo medical center usa summary - Child psychology - Parts of a marketing plan kotler - Reading response at least 300 words - Project strategic - La commedia dell'arte characters - Growing in prayer stephen shead - Passcode for windy city smokeout - Mil g 81827 equivalent - Discusses a contemporary global manufacturing issue related to Industrial & Systems Engineering. - Crime and punishment in the middle ages - Java 2d api graphics pdf - Help with simple essay - 24 merriman street millers point - The financial staff of cairn communications has identified - Abn of hesta super fund - Relation between normality and molarity - Mla citation for young goodman brown - Border collie association nsw - Life cycle of a river - North walsham junior school - Managing organizational change a multiple perspectives approach ppt - Races of destiny 3.5 - OCI & OSC - Calculate the arc elasticity of demand between each point and its neighbor (that is, from A to B, B to C, etc.) and determine whether each value is price elastic, price inelastic, or unit elastic - If the inverse demand function for toasters is - Citric acid and naoh - How does lowest tco differ from lowest purchase price - Non traditional recruitment strategies in south africa - Is italian salad dressing homogeneous or heterogeneous - Blackwood pool werribee gorge - Melting point of urea and cinnamic acid - Dandenong ranges national park walks - Analyzing the article some lessons from the assembly line - NEED SHORT ESSAYS FOR EACH QUESTIONS MINIMUM OF 150 WORDS IN APA FORMAT WITH CITATIONS AND REFERENCES - Homework - Data-driven decision making - What is the goal of imagism - Geometry proof practice worksheet with answers - 98 harewood avenue bournemouth - Assume the following information about the market and jumpmasters stock - Labor Relations - Managerial Economics Assignment - Revitalizing a brand case study - Metanarrative of the bible redemption - I have a dream speech handout - Collision theory gizmo answer key - Abaqus boundary conditions explained - How many trips originate in each state - Finding gold in costa rican cloud forest - Why can't two species occupy the same niche - University of canterbury email - LAUDIUM ABORTION CLINIC +27717852514 ABORTION PILLS FOR SALE IN PRETORIA, ATTERIDGEVILE, SUNNYSIDE, ARCADIA, - Application of double integral - Apprentice performance review template - How have historical public relations events/campaigns shaped current forms of activism? - The crucible act three study questions - Wechsler individual achievement test - Lyrics beds are burning meaning - Habitable zones lab answers - Ibm cognos bi architecture - Emerging threats and countermeasures - Laura langlie literary agent - Question Meaning On My Summer of Scooping Ice Cream - Salt water density tower - Earthworm nervous system dissection - Arabella faded crowned rosette rug