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a. In this equation for specific rotation of a solution: a is the observed rotation in degrees, is the path length in decimetres, c is the concentration in g mL-1, Tis the temperature at which the measurement was taken in °C, and A is the wavelength in nanometres. You used variable path lengths for the polarised light (589 nm) to pass through, and you obtained the fol solutions (A, B, C, D and E) at variable concentrations. observed rotation data at 22°C for five unknown sugar Sugar Path length Sugar concn. Observed rotation 100 50 50 100 50 +0.70 -2.14 -9.20 +2.58 -0.90 10 20 From the table below, what are the most likely identities of the sugars? (For example, A Your sugar choice.) Specific rotation of some sugars at 20 C with the D line of sodium light Specific rotation Fructose Galactose Glucose Lactose Maltose -92.4 +80.2 +52.7 +55.4 +130.5 +14.2 -42.9 +66.5 +18.8 Xylose b. When glucose is dissolved in water, what happens within several hours to the observed rotation of polarised light at 589 nm? And why does this occur? A vineyard worker used a refractometer to measure the sugar concentration in grapes ready for harvest. She got an average refractive index of 1.3740 which means 26% (w/v) sucrose c.
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